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Expression of high mobility group protein B1 in the lungs of rats with sepsis

World Journal of Emergency Medicine 2011年第4期2卷 302-306页

作者： Qiao-meng Qiu Zhong-wang Li Lu-ming Tang Qi Sun Zhong-qiu Lu Huan Liang Guang-liang Hong Meng-fang Li Department of Emergency Medicine First Affi liated Hospital of Wenzhou Medical College Wenzhou 325000 China

BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung ***： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the *** lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi ***： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after *** to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） a

关键词： Vibrio vulnificus Sepsis Lung injury High mobility group protein B1 reversetranscription polymerase chain reaction Western blot Lung water content Histopathology

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Expression pattern of BIM,BCL-6,and c-MYC in adult B-cell acute lymphoblastic leukemia

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Oncology and Translational Medicine 2017年第4期3卷 151-155页

作者： Chanli Zheng Lin Xu Yanjun Xie Dongmei He Yangqiu Li Institute of Hematology Medical CollegeJinan UniversityGuangzhou 510632China Department of Obstetrics and Gynecology Panyu District Zhongcun HospitalGuangzhou 511495China Key Laboratory for Regenerative Medicine of Ministry of Education Jinan UniversityGuangzhou 510632China

Objective We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult patients at initial diagnosis of B-cell acute lymphoblastic leukemia(B-ALL).Methods Relative m RNA levels of BIM, BCL-6, and c-MYC in peripheral blood mononuclear cells(PBMCs) from B-ALL patients were determined by quantitative reverse-transcription polymerase chain reaction(q RT-PCR) using SYBR Green dye. PBMCs from healthy volunteers served as a control. GAPDH was used as a reference *** Relative expression of BIM, BCL-6, and c-MYC m RNA in B-ALL patients was significantly lower than in healthy controls(P 0.05). Spearman's rank correlation analyses revealed the expression level of BIM to be positively correlated with that of BCL-6 in B-ALL *** Expression of the genes BIM, BCL-6, and c-MYC is decreased in adult B-ALL patients. Moreover, the expression pattern of these genes may be similar in such patients at initial diagnosis and following CR. The expression characteristics of BIM, BCL-6, and c-MYC may constitute useful markers for the diagnosis of adult B-ALL.

关键词： BIM BCL-6 c-MYC B cell acute lymphoblastic leukemia (ALL) quantitative reversetranscription polymerase chain reaction (qRT-PCR)

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