Expression pattern of BIM,BCL-6,and c-MYC in adult B-cell acute lymphoblastic leukemia

作     者：Chanli Zheng Lin Xu Yanjun Xie Dongmei He Yangqiu Li 

作者机构：Institute of HematologyMedical CollegeJinan UniversityGuangzhou 510632China Department of Obstetrics and GynecologyPanyu District Zhongcun HospitalGuangzhou 511495China Key Laboratory for Regenerative Medicine of Ministry of EducationJinan UniversityGuangzhou 510632China 

出 版 物：《Oncology and Translational Medicine》 (肿瘤学与转化医学（英文版）)

年 卷 期：2017年第3卷第4期

页      面：151-155页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by the gants of the Guangdong Province Key Foundation of Science and Technology Program(No.2009B0507000029) the Major Research Plan of the National Natural Science Foundation of China(cultivating project,No.91129720) the Guangdong Science&Technology Project(Nos.2012B050600023 and 2015A050502029) a Grant from the Overseas Chinese Affairs Office of the State Council Key Discipline Construction Fund(No.51205002) 

主　　题：BIM BCL-6 c-MYC B cell acute lymphoblastic leukemia (ALL) quantitative reversetranscription polymerase chain reaction (qRT-PCR) 

摘      要：Objective We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult patients at initial diagnosis of B-cell acute lymphoblastic leukemia(B-ALL).Methods Relative m RNA levels of BIM, BCL-6, and c-MYC in peripheral blood mononuclear cells(PBMCs) from B-ALL patients were determined by quantitative reverse-transcription polymerase chain reaction(q RT-PCR) using SYBR Green dye. PBMCs from healthy volunteers served as a control. GAPDH was used as a reference *** Relative expression of BIM, BCL-6, and c-MYC m RNA in B-ALL patients was significantly lower than in healthy controls(P 0.05). Spearman s rank correlation analyses revealed the expression level of BIM to be positively correlated with that of BCL-6 in B-ALL *** Expression of the genes BIM, BCL-6, and c-MYC is decreased in adult B-ALL patients. Moreover, the expression pattern of these genes may be similar in such patients at initial diagnosis and following CR. The expression characteristics of BIM, BCL-6, and c-MYC may constitute useful markers for the diagnosis of adult B-ALL.