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reduning plus ribavirin display synergistic activity against severe pneumonia induced by H1N1 influenza A virus in mice

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Journal of Traditional Chinese Medicine 2020年第5期40卷 803-811页

作者： Chen Weitao Ma Yuexia Zhang Hong Guo Yali Guan Mengyue Wang Yuguang Department of Respiratory Medicine Beijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing 100010China Department of Respiratory Medicine Fangshan HospitalBeijing University of Chinese MedicineBeijing 102400China Department of Internal Medicine Fangshan HospitalBeijing University of Chinese MedicineBeijing 102400China

OBJECTIVE:To investigate synergistic effect of reduning(RDN)injection plus ribavirin against severe pneumonia induced by H1 N1 influenza A virus in ***:We established a mouse model of severe pneumonia induced by influenza A virus by infecting Balb/c mice with CA07 *** randomly assigned the infected mice into four groups,and treated them with normal saline(NS group),RDN(injection,86.6 mg/kg),ribavirin(injection,66.6 mg/kg)or double Ribavirin plus RDN group,the same dosage as used in the single treatments)for 5 *** index and lung pathology were recorded or calculated in terms of the curative ***,NOD-like receptor family pyrin domain containing 3(NLRP3)inflammasome related protein including caspase-associated recruitment domain(CARD)domain Apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC),caspase-1 and NOD-like receptor family,pyrin domain containing 3(NLRP3),and reactive oxygen species were simultaneously ***:RDN plus ribavirin treatment,not RDN or ribavirin alone,provided a significant survival benefit to the influenza A virus-infected *** combination treatment protected the mice against severe influenza infection by attenuating the severe lung *** combined treatment also reduced the viral titers in mouse lungs and lung index,downregulated their immunocytokine levels,including IL-1βand IL-18,and down regulated the NLRP3,especially the transcription and translation of *** NS group had significantly higher reactive oxygen species(ROS)expression which could was dramatically reduced by the treatment of RDN plus ***:Our study showed that RDN combined with ribavirin could protect the mice,and reduce the lung immunopathologic damage caused by severe influenza *** mechanism could be that it reduced ROS produce and inhibited NLRP3 inflammasome activation so that mainly lower the downstream inflammatory cytokines IL-1βand IL-18.

关键词： Influenza A Virus,H1N1 Subtype Pneumonia NLR Family,Pyrin domain-containing 3 protein Inflammasomes Ribavirin reduning

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Antiviral effects of reduning injection against Enterovirus 71 and possible mechanisms of action

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Chinese Journal of Natural Medicines 2015年第12期13卷 881-888页

作者： CAO Ze-Yu CHANG Xiu-Juan ZHAO Zhong-Peng CAO Liang XIAO Wei Jiangsu Kanion Pharmaceutical Co. Ltd. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process Department of Immunology Institute of Microbiology and EpidemiologyAcademy of Military Medical Sciences

The present study was designed to evaluate the protective effects of reduning injection against Enterovirus 71(EV71) in Vero cells and in mice. The Vero cells were infected with 100 and 50 TCID50(50% tissue culture infective dose) of EV71, respectively. The inhibition of reduning injection on cytopathic effect(CPE) was detected. Meanwhile, a mouse model produced by intraperitoneal EV71-infection(106 TCID50), was used to investigate the protective effects of reduning injection. The total survival rate, living time, daily survival rate, weight ratio, and score for symptoms were examined. The viral loads in Vero cells and muscle tissues were detected using real-time PCR. Finally, the content of cytokines was analyzed by ELISA. In the Vero cells, 2.5 mg crude drug·m L-1 of reduning injection inhibited CPE induced by EV71 infection. In the mice, 1.3 g crude drug·kg-1 of reduning injection rescued death triggered by infection, in comparison with model group. Moreover, the survival rate, weight ratio, and clinical scores were also improved. The viral RNA copies in the Vero cells and the mice muscle tissues were reduced. Besides, the steep EV71-induced accumulations of TNF-α and MCP-1 were decreased by reduning injection. In conclusion, reduning injection showed promising protective effects against EV71 in Vero cells and in mice.

关键词： reduning injection EV71 Vero cell Mice Antiviral

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The Mechanism of reduning Injection in the Treatment of COVID-19 associated Myocardial Injury Based on Network Pharmacology and Molecular Docking

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Infectious Diseases Research 2020年第2期1卷 15-24页

作者： Peng Yuan Na Zhang Tianjin University of Traditional Chinese Medicine Tianjin 300193China Baoding NO.1 Hospital of Traditional Chinese Medicine Baoding 071000China

Objective:To explore the potential active components and mechanism of reduning injection in the treatment of coronavirus disease 2019(COVID-19)associated myocardial injury by using molecular docking and network ***:The main chemical constituents and molecular target of reduning injection were collected from *** related to 2019 ncov were screened by *** software was used to construct and analyze the *** active components of reduning injection were molecularly docked with a new coronavirus hydrolase and its binding proteins,angiotensin converting enzyme II(ACE2)and transmembrane serine proteases(TMPRSSs).Results:There were 25 active ingredients and 198 drug targets in reduning injection,43 targets proteins of coronavirus were *** main components have good binding ability with viral hydrolase and related binding ***:reduning injection may intervene the inflammatory response by multi-target and multi-component,inhibit the activity of coronavirus and its binding proteins ACE2 and TMPRSSs,and play a role in the treatment of new coronavirus pneumonia and myocardial injury.

关键词： reduning injection Coronavirus disease 2019(COVID-19) Myocardial injury Network pharmacology Molecular docking Mechanism of action

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