Reduning plus ribavirin display synergistic activity against severe pneumonia induced by H1N1 influenza A virus in mice

作     者：Chen Weitao Ma Yuexia Zhang Hong Guo Yali Guan Mengyue Wang Yuguang Chen Weitao;Ma Yuexia;Zhang Hong;Guo Yali;Guan Mengyue;Wang Yuguang

作者机构：Department of Respiratory MedicineBeijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing 100010China Department of Respiratory MedicineFangshan HospitalBeijing University of Chinese MedicineBeijing 102400China Department of Internal MedicineFangshan HospitalBeijing University of Chinese MedicineBeijing 102400China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2020年第40卷第5期

页      面：803-811页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by The Beijing Natural Science Foundation the research on the mechanisms of Reduning Injection which protects mice from severe pneumonia in terms of the activation level of NLRP3 inflammatomes(No.7172099) 

主　　题：Influenza A Virus,H1N1 Subtype Pneumonia NLR Family,Pyrin domain-containing 3 protein Inflammasomes Ribavirin Reduning 

摘      要：OBJECTIVE:To investigate synergistic effect of Reduning(RDN)injection plus ribavirin against severe pneumonia induced by H1 N1 influenza A virus in ***:We established a mouse model of severe pneumonia induced by influenza A virus by infecting Balb/c mice with CA07 *** randomly assigned the infected mice into four groups,and treated them with normal saline(NS group),RDN(injection,86.6 mg/kg),ribavirin(injection,66.6 mg/kg)or double Ribavirin plus RDN group,the same dosage as used in the single treatments)for 5 *** index and lung pathology were recorded or calculated in terms of the curative ***,NOD-like receptor family pyrin domain containing 3(NLRP3)inflammasome related protein including caspase-associated recruitment domain(CARD)domain Apoptosis-associated speck-like protein containing a caspase recruitment domain(ASC),caspase-1 and NOD-like receptor family,pyrin domain containing 3(NLRP3),and reactive oxygen species were simultaneously ***:RDN plus ribavirin treatment,not RDN or ribavirin alone,provided a significant survival benefit to the influenza A virus-infected *** combination treatment protected the mice against severe influenza infection by attenuating the severe lung *** combined treatment also reduced the viral titers in mouse lungs and lung index,downregulated their immunocytokine levels,including IL-1βand IL-18,and down regulated the NLRP3,especially the transcription and translation of *** NS group had significantly higher reactive oxygen species(ROS)expression which could was dramatically reduced by the treatment of RDN plus ***:Our study showed that RDN combined with ribavirin could protect the mice,and reduce the lung immunopathologic damage caused by severe influenza *** mechanism could be that it reduced ROS produce and inhibited NLRP3 inflammasome activation so that mainly lower the downstream inflammatory cytokines IL-1βand IL-18.