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Profiling the effects of microwave-assisted and soxhlet extraction techniques on the physicochemical attributes of Moringa oleifera seed oil and proteins

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Oil Crop Science 2023年第1期8卷 16-26页

作者： Ngozi Maryann Nebolisa Chukwuebuka Emmanuel Umeyor Uchenna Eunice Ekpunobi Immaculeta Chikamnele Umeyor Festus Basden Okoye Nanomedicines and Drug Delivery Research Group Department of Pharmaceutics and Pharmaceutical TechnologyFaculty of Pharmaceutical SciencesNnamdi Azikiwe UniversityAwka422001Anambra StateNigeria Department of Pure and Industrial Chemistry Faculty of Physical SciencesNnamdi Azikiwe UniversityAwka422001Anambra StateNigeria c Department of Pharmacognosy and Traditional Medicine Faculty of Pharmaceutical SciencesNnamdi Azikiwe UniversityAwka422001Anambra StateNigeria Department of Pharmaceutical and Medicinal Chemistry Faculty of Pharmaceutical SciencesNnamdi Azikiwe UniversityAwka422001Anambra StateNigeria

There is a constant search for biomaterials from natural products like plants for food and industrial applications.The work embodied in this report aimed at investigating the effects of microwave-assisted and soxhlet extraction(MAE and SE) techniques on the functional physicochemical quality characteristics of Moringa oleifera seed oil and proteins extracts. M. oleifera seeds were ground to fine powders and oil was extracted by microwave-assisted and soxhlet extraction techniques using petroleum ether. Quality attributes including yield percent, moisture content,iodine, saponification, specific gravity, viscosity, p H, thiobarbituric acid, acid and peroxide values were measured. Mineral and vitamin contents, chemical/functional groups, fatty acid(FA) composition, and reducing power of the oil were evaluated. Metabolomics of protein extracted from the defatted powders were analyzed by nuclear magnetic resonance(NMR). M. oleifera oil from MAE and SE methods had good yield(34.25 ± 0.0%,28.75 ± 0.0%), low moisture content(0.008 ± 0.0%, 0.011 ± 0.0%), non-drying and unsaturated, moderately saponified, less dense(0.91 ± 0.01, 0.92 ± 0.02 g m L^(-1)), had Newtonian flow, were weakly acidic, showed good content of FAs, recorded strong potential for long shelf-life, showed stability against oxidative rancidity and enzymatic hydrolysis, had very rich deposits of micro-and macro-nutrients as well as water-soluble and lipidsoluble vitamins, and functional groups in the oil were reflective of its content of long-and medium-chain triglycerides(LCT and MCT). Monounsaturated and saturated fatty acids(MUFA and SFA) were detected and the oil has excellent ferric ion reducing power. NMR metabolomic assay revealed the presence of nine essential amino acids(EAAs) in the protein extract. MAE technique is a feasible and acceptable alternative for high throughput extraction of M. oleifera oil with high yield and excellent quality attributes. The study revealed that MAE did not impart any remarkable advantage(s) on the physicochemical pr

关键词： Moringa oleifera seed Oil Microwave-assisted extraction Soxhlet extraction Quality attributes GC-MS assay Metabolomics reducing power

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In Vitro Free Radical Scavenging Activity of Ethanolic Extract of the Whole Plant of Evolvulus Alsinoides(L.) L.

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Chinese Journal of Integrative Medicine 2015年第6期21卷 453-458页

作者： Duraisamy Gomathi Ganesan Ravikumar Manokaran Kalaiselvi Balasubramaniam Vidya Chandrasekar Uma Department of Biochemistry Karpagam University

Objective： To identify the free radical scavenging activity of ethanolic extract of Evolvulus alsinoides. Methods： The free radical scavenging activity was evaluated by in vitro methods like reducing power assay, total antioxidant activity, 2,2-diphenyl-l-picrylhydrazyl （DPPH） reduction, superoxide radical scavenging activity, 2,2＇-azinobis-（3-ethylbenzothiazoline-6-sulfonic acid）（ABTS＊） scavenging activity, hydroxyl radical scavenging assay, and nitric oxide radical scavenging assay, which were studied by using ascorbic acid as standard. Results： The extract showed significant activities in all antioxidant assays compared with the reference antioxidant ascorbic acid. The total antioxidant activity as well as the reducing power was also found to increase in a dose-dependent manner. Conclusion： Evolvulus alsinoides may act as a chemopreventive agent, providing antioxidant properties and offering effective protection from free radicals.

关键词： Evolvulus alsinoides ethanolic extract total antioxidant reducing power ascorbic acid convolvulaceae

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Investigation of hydrolysis conditions and properties on protein hydrolysates from flatfish skin

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Frontiers of Chemical Science and Engineering 2013年第3期7卷 303-311页

作者： Hua ZHANG Hongji ZHU Shipeng WANG Weihua WANG Key Laboratory of Systems Bioengineering (Ministry of Education) School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China

Response surface method （RSM）, based on Box-Behnken design, was used to optimize the enzymatic hydrolysis conditions of flatfish skin protein hydrolysates （FSPH）. Among the tested proteases, the combination of nutrase and trypsin was selected. The optimal hydrolysis conditions were as follows： pH 7.3, temperature 51.8℃, and the enzyme/substrate （E/S） ratio 2.5; under these conditions, the maximum peptide yield （PY） was 69.41 =1：0.43%. The physiochemical analysis showed that the amino acids （His, Asp and Glu） of FSPH accounted for 18.15%, and FSPH was a mixture of polypeptides mostly distributed among 900-2000 Da. FSPH could exhibit a 93% chelating effect on ferrous ion at a concentration of 400 gg/mL, and also a notable reducing power. This study showed bioprocess for the production of FSPH for the first time, which had a good potential for valuable ingredients in the food, cosmetic and medicine industries.

关键词： flatfish skin protein hydrolysates metal chelating activity reducing power

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Assessments of Antibacterial and Antioxidant Properties in the Methanolic and Aqueous Leaf Extracts of Pistacia lentiscus against Different Antibiotic Resistance Pathogenic Bacteria

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Advances in Bioscience and Biotechnology 2022年第3期13卷 113-133页

作者： Aml O. Alhadad Galal S. Salem Maraia F. Elmhdwi Suliman M. Hussein Sarah M. Elshareef Department of Botany Faculty of Science University of Benghazi Benghazi Libya Department of Chemistry (Biochemistry) Faculty of Science University of Benghazi Benghazi Libya

The current study was carried out to determine the bioactivity of P. lentiscus leaf extracts as potential antibacterial and antioxidant properties. The plant extracts were examined for antibacterial activity against antibiotic-resistant Staphylococcus aureus, Staphylococcus haemolyticus, Pseudomonas aeruginosa, and Proteus mirabilis using the agar well method (according to the guidelines of Clinical and Laboratory Standard Institute). The antioxidant potential of 3 plant leaf extracts was determined by their ability to convert Fe3+ to Fe2+ and scavenge the DPPH free radical. At all concentrations studied, the methanolic leaf extract had higher total phenolic and flavonoid content, as well as stronger antioxidant and antibacterial inhibitory activity compared to aqueous extract. Our findings with P. aeruginosa were especially interesting, because this bacterium was inhibited by methanol extract than that of the reference antibiotics. The results also demonstrated a link between DPPH radical scavenging ability, reducing power, and total phenolic and flavonoid content of plant extracts (r > 0.97, R2 > 0.95, P = 0.01). As a result, the methanolic leaf extract of the chosen plant might be employed as an effective antioxidant and antibacterial agent for the treatment of a variety of morbidities.

关键词： Antioxidant Activity Polyphenols Methanolic Leaf Extracts DPPH reducing power Pistacia lentiscus

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Phytochemical Screening, Extraction of Essential Oils and Antioxidant Activity of Five Species of Unconventional Vegetables

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American Journal of Plant Sciences 2015年第16期6卷 2632-2639页

作者： Marcos Schleiden Sousa Carvalho Maria das Gracas Cardoso Luciane Vilela Resende Marcos de Souza Gomes Luiz Roberto Marques Albuquerque Anni Cristini Silvestri Gomes Thaís Aparecida Sales Karen Caroline Camargo David Lee Nelson Gabriele Mikami Costa Mariana Araújo Espósito Luis Felipe Lima e Silva Department of Agriculture Federal University of Lavras Lavras Brazil Department of Chemistry Federal University of Lavras Lavras Brazil Federal University of Vales de Jequitinhonha e Mucuri Unaí Brazil Federal University of Vales de Jequitinhonha e Mucuri Diamantina Brazil

Unconventional vegetables, in general, are plants that have been largely consumed by the population at some point and, because of changes in eating behavior, now present reduced economic and social expression and have lost ground to other vegetables. The objectives of this study were to perform phytochemical screening of the ethanol extracts of Rumex acetosa L., Tropaeolum majus L., Solanum muricatum, Stachys byzantina K. Koch and Solanum betaceum Cav. and evaluate their antioxidant potentials via the methods involving scavaging of the DPPH free radical and the ABTS radical, phosphomolybdenum and reducing power. In phytochemical screening, five species of unconventional vegetables tested positive for tannins;for sesquiterpene, lactones and other lactones. These tests were positive for Tropaeolum majus L. and Rumex acetosa L., Solanum betaceum Cav. and Solanum muricatum tested negative for steroids. Only Solanum betaceum Cav. gave positive tests for flavonoids. Among the five plant species studied, Stachys byzantina K. Koch presented the greatest antioxidant potential in all the methods evaluated.

关键词： Rumex acetosa L. Tropaeolum majus L. Stachys byzantina K.Koch reducing power

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Anti-melanogenic Activities of Collagen Peptides from Jellyfish （Stomolophus meleagris）

Anti-melanogenic Activities of Collagen Peptides from Jellyf...

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2010 First International Conference on Cellular,Molecular Biology, Biophysics and Bioengineering(CMBB 2010)

作者： Zhuang Yongliang and Sun Liping College of Chemistry and Engineering Kunming University of Science and Technology Kunming Yunnan 650224 China

Collagen was extracted from jellyfish （Stomolophus meleagris） and hydrolyzed to prepare collagen peptides. Three fractions （JCP-1＜1kDa, 1kDa＜JCP-2＜3kDa, JCP-3＞3kDa） were collected by ultrafiltration from jellyfish collagen peptides and inhibitory abilities of melanogenesis were evaluated using cultured B16 melanoma cells. The results showed three fractions could inhibit intracellular tyrosinase activity, decrease melanin content and enhance glutathione synthesis. Furthermore, they decreased intracellular cAMP levels and suppressed tyrosinase mRNA expression. JCP-2 showed much higher anti-melanogeneic activities than JCP-1 and JCP-3 because of its amino acids composition and molecular weight distribution. Our results indicated JCP-2 could be used as a natural skin-lightening agent in the medicine and food industries.

关键词： melanin collagen tyrosinase reducing power Stomolophus meleagris

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Total Flavonoids and Antioxidant Activity in Vitro of Extracts of Taxillus chinensis Parasitizing on Two Different Hosts

Total Flavonoids and Antioxidant Activity in Vitro of Extrac...

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多学科交叉，产学研联合，实现西部药用植物资源的可持续利...

作者： Lini Huo,Peiyuan Li,Rumei Lu,Chaocheng Deng,Youguo Lv, Pengying Liao,Quan Liu College of Pharmacy,Guangxi traditional Chinese Medical University, Nanning 530001,China

Parasites are unusal plants,more than 2500 species of higher plants are known to live parasitically on other plants.Taxillus chinensis is belonging to the Loranthaceae family,distributed in Guangdong, Guangxi,Tanwan and Fujian province in china.Some common host trees of T.chinensis are mulberry,plum trees,longan trees,lychee trees and oleander trees,etc.Preparations from leaves and stems of this plant are used folkloric medicine in the treatment of pain in rheumatism,lack of energy

关键词： Total flavonoids content DPPH ABTS reducing power Taxillus chinensis

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Analysis of Prote in Composition and Antioxidant Activity of Hydrolysates from Paphia undulata

Analysis of Prote in Composition and Antioxidant Activity of...

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广东省食品学会2013年年会暨学会成立30周年纪念大会

作者： HE Xiao-qing CAO Wen-hong ZHANG Chao-hua ZHAO Zi-ke Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education InstitutionNational Research and Development Branch Center for Shellfish Processing(Zhanjiang)College of Food Science and TechnologyGuangdong Ocean University

Three types of proteins including sarcoplasmic protein,myofibrillar protein and stromal protein were isolated and from Paphia undulata muscle tissue.Myofibrillar protein had the highest content(45.42%),followed by sarcoplasmic protein(31.20%) and stromal protein(20.69%).Analysis of DSC found that the denaturation temperatures of sarcoplasmic protein,myofibrillar protein and stromal protein were 53.4℃,47.23℃and 55.73℃,respectively.The three protein fractions contained higher contents essential amino acids,alkaline,hydrophobic and branched chain amino acid.Protein hydrolysates prepared with trypsin revealed a good radical scavenging activity.Moreover,the hydrolysates of sarcoplasmic protein exhibited the strongest radical scavenging activities including DPPH radical,hydroxyl radical and superoxide anion radical,with the IC values being 4.82,4.70 and 3.75 mg/mL,respectively.In addition,the hydrolysates of sarcoplasmic protein and myofibrillar protein showed a similar reducing power and their reducing power were 0.58 and 0.57 at 10 mg/mL,respectively.While the hydrolysates of stromal protein displayed lower reducing power.

关键词： protein isolation DSC protein hydrolysate free radical scavenging activity reducing power

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