Profiling the effects of microwave-assisted and soxhlet extraction techniques on the physicochemical attributes of Moringa oleifera seed oil and proteins

作     者：Ngozi Maryann Nebolisa Chukwuebuka Emmanuel Umeyor Uchenna Eunice Ekpunobi Immaculeta Chikamnele Umeyor Festus Basden Okoye Ngozi Maryann Nebolisa;Chukwuebuka Emmanuel Umeyor;Uchenna Eunice Ekpunobi;Immaculeta Chikamnele Umeyor;Festus Basden Okoye

作者机构：Nanomedicines and Drug Delivery Research GroupDepartment of Pharmaceutics and Pharmaceutical TechnologyFaculty of Pharmaceutical SciencesNnamdi Azikiwe UniversityAwka422001Anambra StateNigeria Department of Pure and Industrial ChemistryFaculty of Physical SciencesNnamdi Azikiwe UniversityAwka422001Anambra StateNigeria c Department of Pharmacognosy and Traditional MedicineFaculty of Pharmaceutical SciencesNnamdi Azikiwe UniversityAwka422001Anambra StateNigeria Department of Pharmaceutical and Medicinal ChemistryFaculty of Pharmaceutical SciencesNnamdi Azikiwe UniversityAwka422001Anambra StateNigeria 

出 版 物：《Oil Crop Science》 (中国油料作物学报(英文版）)

年 卷 期：2023年第8卷第1期

页      面：16-26页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：funded by International Foundation for Science(IFS)and Organisation for the Prohibition of Chemical Weapons(OPCW)research grant awarded to Dr.Chukwuebuka Emmanuel Umeyor in 2019(Grant number:I-2-F-6448-1) 

主　　题：Moringa oleifera seed Oil Microwave-assisted extraction Soxhlet extraction Quality attributes GC-MS assay Metabolomics Reducing power 

摘      要：There is a constant search for biomaterials from natural products like plants for food and industrial *** work embodied in this report aimed at investigating the effects of microwave-assisted and soxhlet extraction(MAE and SE) techniques on the functional physicochemical quality characteristics of Moringa oleifera seed oil and proteins extracts. M. oleifera seeds were ground to fine powders and oil was extracted by microwave-assisted and soxhlet extraction techniques using petroleum ether. Quality attributes including yield percent, moisture content,iodine, saponification, specific gravity, viscosity, p H, thiobarbituric acid, acid and peroxide values were measured. Mineral and vitamin contents, chemical/functional groups, fatty acid(FA) composition, and reducing power of the oil were evaluated. Metabolomics of protein extracted from the defatted powders were analyzed by nuclear magnetic resonance(NMR). M. oleifera oil from MAE and SE methods had good yield(34.25 ± 0.0%,28.75 ± 0.0%), low moisture content(0.008 ± 0.0%, 0.011 ± 0.0%), non-drying and unsaturated, moderately saponified, less dense(0.91 ± 0.01, 0.92 ± 0.02 g m L^(-1)), had Newtonian flow, were weakly acidic, showed good content of FAs, recorded strong potential for long shelf-life, showed stability against oxidative rancidity and enzymatic hydrolysis, had very rich deposits of micro-and macro-nutrients as well as water-soluble and lipidsoluble vitamins, and functional groups in the oil were reflective of its content of long-and medium-chain triglycerides(LCT and MCT). Monounsaturated and saturated fatty acids(MUFA and SFA) were detected and the oil has excellent ferric ion reducing power. NMR metabolomic assay revealed the presence of nine essential amino acids(EAAs) in the protein extract. MAE technique is a feasible and acceptable alternative for high throughput extraction of M. oleifera oil with high yield and excellent quality attributes. The study revealed that MAE did not impart any r