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Hif-1α/Hsf1/Hsp70 signaling pathway regulates redox homeostasis and apoptosis in large yellow croaker(Larimichthys crocea)under environmental hypoxia

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Zoological Research 2021年第6期42卷 746-760页

作者： Sheng-Yu Luo Jing-Qian Wang Cheng Liu Xin-Ming Gao Yi-Bo Zhang Jie Ding Cong-Cong Hou Jun-Quan Zhu Bao Lou Wei-Liang Shen Xiong-Fei Wu Chun-Dan Zhang Dao-Jun Tang Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingboZhejiang 315211China Zhejiang Academy of Agricultural Sciences HangzhouZhejiang 310021China State Key Laboratory of Large Yellow Croaker Breeding Ningbo Academy of Oceanology and FisheryNingboZhejiang 315012China

Oxygen is an essential molecule for animal respiration,growth,and *** in terrestrial environments,contamination and climate change have led to the frequent occurrence of hypoxia in aquatic environments,thus impacting aquatic animal ***,the adaptative mechanisms underlying fish responses to environmental hypoxia remain largely ***,we used large yellow croaker(Larimichthys crocea)and large yellow croaker fry(LYCF)cells to investigate the roles of the Hif-1α/Hsf1/Hsp70 signaling pathway in the regulation of cellular redox homeostasis,and *** confirmed that hypoxia induced the expression of Hif-1α,Hsf1,and Hsp70 in vivo and in *** Hsp70 knockdown/overexpression indicated that Hsp70 was required for maintaining redox homeostasis and resisting oxidative stress in LYCF cells under hypoxic ***70 inhibited caspase-dependent intrinsic apoptosis by maintaining normal mitochondrial membrane potential,enhancing Bcl-2 mRNA and protein expression,inhibiting Bax and caspase3 mRNA expression,and suppressing caspase-3 and caspase-9 ***70 suppressed caspaseindependent intrinsic apoptosis by inhibiting nuclear translocation of apoptosis-inducing factor(AIF)and disturbed extrinsic apoptosis by inactivating *** knockdown/overexpression of Hif-1αand dual-luciferase reporter assay indicated that Hif-1αactivated the Hsf1 DNA promoter and enhanced Hsf1 mRNA ***1 enhanced Hsp70 mRNA transcription in a similar *** summary,the Hif-1α/Hsf1/Hsp70 signaling pathway plays an important role in regulating redox homeostasis and anti-apoptosis in *** under hypoxic stress.

关键词： Hypoxia Larimichthys crocea Apoptosis redox homeostasis Hif-1α/Hsf1/Hsp70

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Current Opinions on the Functions of Tocopherol Based on the Genetic Manipulation of Tocopherol Biosynthesis in Plants

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Journal of Integrative Plant Biology 2008年第9期50卷 1057-1069页

作者： Yin Li Zinan Wang Xiaofen Sun Kexuan Tang State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Fudan University Shanghai 200433 China Plant Biotechnology Research Center School of Agriculture and Biology Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Shanghai Jiao Tong University Shanghai 200030 China

As a member of an important group of lipid soluble antioxidants, tocopherols play a paramount role in the daily diet of humans and animals. Recently, genes required for tocochromanol biosynthesis pathway have been identified and cloned with the help of genomics-based approaches and molecular manipulation in the model organisms： Arabidopsis thaliana and Synechocystis sp. PCC 6803. At the basis of these foundations, genetic manipulation of tocochromanol biosynthesis pathway can give rise to strategies that enhance the level of tocochromanol content or convert the constitution of tocochromanol. In addition, genetic manipulations of the tocochromanol biosynthesis pathway provide help for the study of the function of tocopherol in plant systems. The present article summarizes recent advances and pays special attention to the functions of tocopherol in plants. The roles of tocopherol in the network of reactiv.e oxygen species, antioxidants and phytohormones to maintain redox homeostasis and the functions of tocopherol as a signal molecule in chloroplast-to- nucleus signaling to regulate carbohydrate metabolism are also discussed.

关键词： antioxidant carbohydrate metabolism genetic dissection programmed cell death redox homeostasis signal molecule tocopherol.

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GmGPDH12,a mitochondrial FAD-GPDH from soybean, increases salt and osmotic stress resistance by modulating redox state and respiration

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The Crop Journal 2021年第1期9卷 79-94页

作者： Ying Zhao Xin Li Zexin Zhang Wenjing Pan Sinan Li Yun Xing Wanying Xin Zhanguo Zhang Zhenbang Hu Chunyan Liu Xiaoxia Wu Zhaoming Qi Dawei Xin Qingshan Chen College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina Key Lab of Maize Genetics and Breeding Heilongjiang Academy of Agricultural SciencesHarbin 150030HeilongjiangChina College of Agriculture Heilongjiang Bayi Agricultural UniversityDaqing 163319HeilongjiangChina

In plants,glycerol-3-phosphate dehydrogenase(GPDH)catalyzes the interconversion of glycerol-3-phosphate(G3P)and dihydroxyacetone phosphate(DHAP)coupled to the reduction/oxidation of the nicotinamide adenine dinucleotide(NADH)pool,and plays a central role in glycerolipid metabolism and stress *** studies have focused mainly on the NAD+-dependent GPDH isoforms,neglecting the role of flavin adenine dinucleotide(FAD)-dependent *** isolated and characterized three mitochondrialtargeted FAD-GPDHs in soybean,of which one isoform(GmGPDH12)showed a significant transcriptional response to NaCl and mannitol treatments,suggesting the existence of a major FAD-GPDH isoform acting in soybean responses to salt and osmotic *** enzyme kinetic assay showed that the purified GmGPDH12 protein possessed the capacity to oxidize G3P to DHAP in the presence of *** and RNA interference of GmGPDH12 in soybean hairy roots resulted in elevated tolerance and sensitivity to salt and osmotic stress,respectively.G3P contents were significantly lower in GmGPDH12-overexpressing hair roots and higher in knockdown hair roots,indicating that GmGPDH12 was essential for G3P catabolism.A significant perturbation in redox status of NADH,ascorbic acid(ASA)and glutathione(GSH)pools was observed in GmGPDH12-knockdown plants under stress *** impaired redox balance was manifested by higher reactive oxygen species generation and consequent cell damage or death;however,overexpressing plants showed the opposite results for these ***12 overexpression contributed to maintaining constant respiration rates under salt or osmotic stress by regulating mRNA levels of key mitochondrial respiratory *** study provides new evidence for the roles of mitochondria-localized GmGPDH12 in conferring resistance to salt or osmotic stress by maintaining cellular redox homeostasis,protecting cells and respiration from oxidative injury.

关键词： redox homeostasis Respiration characteristics GmGPDH12 Salt stress Osmotic stress

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Activated PKB/GSK-3β synergizes with PKC-δ signaling in attenuating myocardial ischemia/reperfusion injury via potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-Ⅰ

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Acta Pharmaceutica Sinica B 2021年第1期11卷 71-88页

作者： Hao Zengy Lingling Wangy Jiawei Zhang Ting Pan Yinghua Yu Jingxia Lu Ping Zhou Hua Yang Ping Li State Key Laboratory of Natural Medicines School of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjing 210009China

Disrupted redox status primarily contributes to myocardial ischemia/reperfusion injury(MIRI).NRF2,the endogenous antioxidant regulator,might provide therapeutic ***-Ⅰ(DT)is an active component in Salvia miltiorrhiza with NRF2 induction *** study seeks to validate functional links between NRF2 and cardioprotection of DT and to investigate the molecular mechanism particularly emphasizing on NRF2 cytoplasmic/nuclear *** potently induced NRF2 nuclear accumulation,ameliorating post-reperfusion injuries via redox *** cardioprotection in NRF2-deficient mice and cardiomyocytes strongly supports NRF2-dependent cardioprotection of ***,DT phosphorylated NRF2 at Ser40,rendering its nuclear-import by dissociating from KEAP1 and inhibiting ***,we identified PKC-δ-(Thr505)phosphorylation as primary upstream event triggering NRF2-(Ser40)*** of PKC-δdramatically retained NRF2 in cytoplasm,convincing its pivotal role in mediating NRF2 ***2 activity was further enhanced by activated PKB/GSK-3βsignaling via nuclear-export signal blockage independent of PKC-δ*** demonstrating independent modulation of PKC-δand PKB/GSK-3β/Fyn signaling,we highlight the ability of DT to exploit both nuclear import and export regulation of NRF2 in treating reperfusion injury harboring redox homeostasis *** of PKC and PKB phenocopied cardioprotection of DT in vitro and in vivo,further supporting the potential applicability of this rationale.

关键词： DihydrotanshinoneⅠ NRF2 Cytoplasmic/nuclear translocation PKC-δ PKB/GSK-3β/Fyn Phosphorylation redox homeostasis Ischemia/reperfusion injury

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Irisin-loaded electrospun core-shell nanofibers as calvarial periosteum accelerate vascularized bone regeneration by activating the mitochondrial SIRT3 pathway

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Regenerative Biomaterials 2024年第1期3卷 44-57页

作者： Xi Hua Mingzhuang Hou Lei Deng Nanning Lv Yong Xu Xuesong Zhu Huilin Yang Qin Shi Hao Liu Fan He Department of Orthopaedics The First Affiliated Hospital of Soochow UniversitySoochow UniversitySuzhou 215006China Orthopaedic Institute Suzhou Medical CollegeSoochow UniversitySuzhou 215000China Department of Orthopedics Suzhou Wuzhong People’s HospitalSuzhouJiangsu Province 215128China Department of Orthopedic Surgery Lianyungang Clinical College of Xuzhou Medical UniversityLianyungang 222003China

The scarcity of native periosteum poses a significant clinical barrier in the repair of critical-sized bone *** challenge of enhancing regenerative potential in bone healing is further compounded by oxidative stress at the fracture ***,the introduction of artificial periosteum has demonstrated its ability to promote bone regeneration through the provision of appropriate mechanical support and controlled release of proosteogenic *** this study,a poly(L-lactic acid)(PLLA)/hyaluronic acid(HA)-based nanofibrous membrane was fabricated using the coaxial electrospinning *** incorporation of irisin into the core-shell structure of PLLA/HA nanofibers(PLLA/HA@Irisin)achieved its sustained *** vitro experiments demonstrated that the PLLA/HA@Irisin membranes exhibited favorable *** osteogenic differentiation of bone marrow mesenchymal stem cells(BMMSCs)was improved by PLLA/HA@Irisin,as evidenced by a significant increase in alkaline phosphatase activity and matrix ***,PLLA/HA@Irisin significantly enhanced the mitochondrial function of BMMSCs via the activation of the sirtuin 3 antioxidant *** assess the therapeutic effectiveness,PLLA/HA@Irisin membranes were implanted in situ into critical-sized calvarial defects in *** results at 4 and 8 weeks post-surgery indicated that the implantation of PLLA/HA@Irisin exhibited superior efficacy in promoting vascularized bone formation,as demonstrated by the enhancement of bone matrix synthesis and the development of new blood *** results of our study indicate that the electrospun PLLA/HA@Irisin nanofibers possess characteristics of a biomimetic periosteum,showing potential for effectively treating critical-sized bone defects by improving the mitochondrial function and maintaining redox homeostasis of BMMSCs.

关键词： periosteum irisin mitochondrial function critical-sized bone defect redox homeostasis

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Mitochondrial GPX1 silencing triggers differential photosynthesis impairment in response to salinity in rice plants

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Journal of Integrative Plant Biology 2016年第8期58卷 737-748页

作者： Yugo Lima-Melo Fabricio E.L.Carvalho Márcio O.Martins Gisele Passaia Rachel H.V.Sousa Milton C.Lima Neto Márcia Margis-Pinheiro Joaquim A.G.Silveira Department of Biochemistry and Molecular Biology Federal University of Ceará Department of Genetics Federal University of Rio Grande do Sul

The physiological role of plant mitochondrial glutathione peroxidases is scarcely known. This study attempted to elucidate the role of a rice mitochondrial isoform（GPX1） in photosynthesis under normal growth and salinity conditions. GPX1 knockdown rice lines（GPX1s） were tested in absence and presence of 100 mM NaCl for 6 *** reduction of GPX1 s line under non-stressful conditions, compared with non-transformed（NT） plants occurred in parallel to increased H_2O_2 and decreased GSH contents. These changes occurred concurrently with photosynthesis impairment, particularly in Calvin cycle＇s reactions, since photochemical efficiency did not ***, GPX1 silencing and downstream molecular/metabolic changes modulated photosynthesis differentially. In contrast, salinity induced reduction in both phases of photosynthesis, which were more impaired in silenced *** changes were associated with root morphology alterations but not shoot growth. Both studied lines displayed increased GPX activity but H_2O_2 content did not change in response to salinity. Transformed plants exhibited lower photorespiration, water use efficiency and root growth, indicating that GPX1 could be important to salt tolerance. Growth reduction of GPX1 s line might be related to photosynthesis impairment, which in turn could have involved a cross talk mechanism between mitochondria and chloroplast originated from redox changes due to GPX1 deficiency.

关键词： CO2 assimilation glutathione peroxidase Oryza sativa photochemistry redox homeostasis

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Changes of oxidative metabolism in the roots of wheat(Triticum aestivum L.)seedlings in response to elevated ammonium concentrations

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Journal of Integrative Agriculture 2021年第5期20卷 1216-1228页

作者： LIU Yang LI Yu-xiang LI Yi-xiang TIAN Zhong-wei HU Jin-ling Steve ADKINS DAI Ting-bo Key Laboratory of Crop Physiology and Ecology in Southern China/College of Agriculture Nanjing Agricultural UniversityNanjing 210095P.R.China College of Agriculture Shihezi UniversityShihezi 823003P.R.China School of Agriculture and Food Sciences the University of QueenslandGatton QLD 4343Australia

To elucidate the response of oxidative metabolism,triggered by elevated ammonium(NH_(4)^(+))concentrations,on root growth of wheat seedlings,Yumai 49(NH_(4)^(+)-tolerant)and Lumai 15(NH_(4)^(+)-sensitive)cultivars were supplied with either 5.0 mmol L^(–1)NH_(4)^(+)-N(EAC)or 5.0 mmol L–1 NO_(3)–-N(CON)under hydroponic *** growth in both cultivars was significantly reduced under EAC,and the negative effect was greater in Lumai *** enhanced the activities of monodehydroascorbate reductase and dehydroascorbate reductase in the roots of both cultivars,while it decreased ascorbic acid(ASA)content and GDP-mannose pyrophosphorylase(GMPase)activity at the 12 th day after treatment in Lumai 15 by 62.0 and 71.4%;and in Yumai 49 by 38.8 and 62.2%,respectively,indicating that the regeneration of ASA was increased,but the biosynthesis of ASA was reduced under EAC ***,EAC increased DHA/ASA,reactive oxygen species(ROS),and malondialdehyde contents,as well as antioxidant enzyme activities in the roots of both *** greater increases in ROS and soluble sugar,and lower antioxidant enzyme activities in Lumai 15 indicate severe disruption of oxidative metabolism when compared to Yumai *** reveal that the reduction of ASA biosynthesis via decreased GMPase activity under the EAC condition probably acts as a trigger for accumulated ROS and imbalanced redox status,resulting in root growth inhibition during wheat seedling growth *** 49,being an NH_(4)^(+)-tolerant cultivar,had the stronger capacity to protect itself from oxidative stress,which allowed it to retain a lower DHA to ASA ratio by maintaining a better redox homeostasis than could be maintained in the NH_(4)^(+)-sensitive cultivar Lumai 15.

关键词： elevated ammonium concentrations oxidative metabolism redox homeostasis root morphology wheat

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SPINDLY, ERECTA, and Its Ligand STOMAGEN Have a Role in redox-Mediated Cortex Proliferation in the Arabidopsis Root

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Molecular Plant 2014年第12期7卷 1727-1739页

作者： Hongchang Cui Danyu Konga Pengcheng Wei Yueling Hao Keiko U. Torii Jin Suk Lee Jie Li Department of Biological Science Florida State University Tallahassee FL 32306-4295 USA Present address: Biotechnical Group Institute of Rice Research Anhui Agricultural Academy of Science 40# Nongke South RoadHefei Anhui 230031 China Howard Hughes Medical Institute Department of Biology University of Washington Seattle WA 98195-1800 USA

Reactive oxygen species （ROS） are harmful to all living organisms and therefore they must be removed to ensure normal growth and development. ROS are also signaling molecules, but so far little is known about the mecha- nisms of ROS perception and developmental response in plants. We here report that hydrogen peroxide induces cortex proliferation in the Arabidopsis root and that SPINDLY （SPY）, an O-linked glucosamine acetyltransferase, regulates cortex proliferation by maintaining cellular redox homeostasis. We also found that mutation in the leucine-rich receptor kinase ERECTA and its putative peptide ligand STOMAGEN block the effect of hydrogen peroxide on root cortex proliferation. However, ERECTA and STOMAGEN are expressed in the vascular tissue, whereas extra cortex cells are produced from the endodermis, suggesting the involvement of intercellular signaling~ SPY appears to act downstream of ERECTA, because the spy mutation still caused cortex proliferation in the erecta mutant background. We therefore have not only gained insight into the mechanism by which SPY regulates root development but also uncovered a novel pathway for ROS signal- ing in plants. The importance of redox-mediated cortex proliferation as a protective mechanism against oxidative stress is also discussed.

关键词： SPY ERECTA STOMAGEN redox homeostasis ROS signaling abiotic stress cortex proliferation Arabidopsisthaliana.

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LSL1 controls cell death and grain production by stabilizing chloroplast in rice

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Science China(Life Sciences) 2022年第11期65卷 2148-2161页

作者： Deyong Ren Wei Xie Qiankun Xu Jiang Hu Li Zhu Guangheng Zhang Dali Zeng Qian Qian State Key Lab of Rice Biology China National Rice Research InstituteHangzhou 310006China College of Modern Agriculure Zhejiang A&F UniversityHangzhou 31006China National Nanfan Research Institute Chinese Academy of Agricultural SciencesSanya 572024China

Lesion mutants can be valuable tools to reveal the interactions between genetic factors and environmental signals and to improve grain *** we identified a rice(Oryza sativa)mutant,lesion spotleaf1(lsl1),which produces necrotic leaf lesions throughout its life ***1 encodes a protein of unknown function and belongs to a grass-specific *** lesion phenotype of the lsl1 mutant was sharply induced by shading,and its detached leaves incubated in 6-benzylamino purine similarly formed lesions in the *** addition,the lsl1 mutant exhibited reactive oxygen species accumulation and cell *** terminal deoxynucleotidyl transferase d UTP nick end-labeling(TUNEL)and comet assays revealed that the lsl1 mutant contained severe DNA damage,resulting in reduced grain yield and *** sequencing,gene expression,and protein activity analyses indicate that LSL1 is required for chloroplast ***,LSL1 interacts with Psa D and PAP10 to form a regulatory module that functions in chlorophyll synthesis and chloroplast development to maintain redox *** results reveal that LSL1 maintains chloroplast structure,redox homeostasis,and DNA stability,and plays important roles in the interaction between genetic factors and environmental signals and in regulating grain size and quality.

关键词： lesion formation chloroplast function redox homeostasis grain size and quality LSL1 rice

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Dusp 1 regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity

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Zoological Research 2023年第1期44卷 126-141页

作者： Ying Wang Hua-Min Wang Yan Zhou Ling-Hong Hu Jing-Ming Wan Ji-Hui Yang Hong-Bo Niu Xiu-Ping Hong Peng Hu Liang-Biao Chen International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai 200120China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai 200120China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai 200120China

Temperature tolerance restricts the distribution of a species. However, the molecular and cellular mechanisms that set the thermal tolerance limits of an organism are poorly understood. Here, we report on the function of dual-specificity phosphatase 1(DUSP1) in thermal tolerance regulation. Notably, we found that dusp1-/- zebrafish grew normally but survived within a narrowed temperature range. The higher susceptibility of these mutant fish to both cold and heat challenges was attributed to accelerated cell death caused by aggravated mitochondrial dysfunction and over-production of reactive oxygen species in the gills. The DUSP1-MAPK-DRP1 axis was identified as a key pathway regulating these processes in both fish and human cells. These observations suggest that DUSP1 may play a role in maintaining mitochondrial integrity and redox homeostasis. We therefore propose that maintenance of cellular redox homeostasis may be a key mechanism for coping with cellular thermal stress and that the interplay between signaling pathways regulating redox homeostasis in the most thermosensitive tissue(i.e., gills) may play an important role in setting the thermal tolerance limit of zebrafish.

关键词： redox homeostasis Zebrafish Ectothermal Mitochondrial fission Thermal stress MAPK

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