检索结果-南通市图书馆

ras protein participated in histone acetylation-mediated cell cycle control in Physarum polycephalum

Science Bulletin 2005年第16期 51-55页

作者： LI Xiaoxue, LU Jun, ZHAO Yanmei, WANG Xiuli & HUANG Baiqu Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China

In this paper, we demonstrate that in Physarum polycephalum, a naturally synchronized slime mold, histone deacetylase (HDAC) inhibitor Trichostatin A (TSA), arrestes the cell cycle at the checkpoints of S/G2, G2/M and mitosis exit, and influences the transcription of two ras genes Ppras1 and Pprap1, as well as the ras protein level. Antibody neu-tralization experiment using anti-ras antibody treatment showed that ras protein played an important role in cell cycle checkpoint control through regulation of the level of Cyclin B1, suggesting that ras protein might be a key factor for histone acetylation-mediated cell cycle regulation in P. polycephalum.

关键词： ras protein histone acetylation Trichostatin A (TSA) cell cycle regulation Physarum polycephalum.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Engineering a cell-penetrating hyperstable antibody scFv(ras)-An extraordinary approach to cancer therapeutics

引用

Synthetic and Systems Biotechnology 2021年第4期6卷 343-350页

作者： Jina Bae Yoonyee Song Chadwick International Yeonsu-guIncheon22002South Korea Lansing Catholic High School LansingMI48912USA

In the modern pharmaceutical industry,monoclonal antibodies are often used as therapeutic ***,they are restricted to cell surface antigens due to their inability to penetrate the outer cell membrane and maintain normal function in the reducing ***,it can lead to cytotoxicity since it attacks cancerous cells by mimicking the human immune *** an alternative,this study modifies the hyperstable single-chain fragment variable(scFv)antibody to eliminate cancer using its linear *** scFv(F8)antibody model was modified to recognize human ras protein by altering residues in the antigen-binding ***,a cell-penetrating peptide(CPP)was attached to the scFv(ras)antibody model to allow entrance to the cell,creating CPP-scFv(ras).Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE),western blotting,and the binding assay were performed to prove its *** a result,CPP-scFv(ras)was successfully engineered and bound to the antigen,Hras(G12V).

关键词： Hyperstable Single chain variable fragment antibody Cell-penetrating peptide ras protein Cancer therapeutics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Impact of Mutations on K-ras-p120GAP Interaction

引用

Computational Molecular Bioscience 2013年第2期3卷 9-17页

作者： Chunxia Gao Leif A.Eriksson Department of Chemistry and Molecular Biology University of GothenburgGoteborgSweden

The K-ras protein plays a key role in the signal transduction cascade. Certain mutations in K-ras lead to a permanent “on” state which results in tumorigenesis due to failed interaction with the GTPase activating protein (GAP). In this study, we examined the mutations E31N, D33N and D38N of K-ras coupled and decoupled to wildtype GAP-334 and mutation K935N of GAP-334 coupled and decoupled to wildtype K-ras, to illustrate the potential mechanism by which these mutants affect the interaction between the two proteins. We identify Tyr32 in the ras Switch I region as a critical residue that acts as a gate to the GTP binding site and which needs to be “open” during ras coupling with GAP to allow for insertion of GAP residue Arg789. This residue plays a vital role in stabilizing the transition state during GTP hydrolysis. The different mutations studied herein caused a reduced binding affinity, and the fluctuation of the Tyr32 side chain might hinder the insertion of Arg789. This may in turn be the cause of decreased GTP hydrolysis, and permanent “on” state of K-ras, observed for these mutants.

关键词： ras protein GTPase Activating protein Molecular Dynamics Simulations In Silico Mutation Studies Cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：