Engineering a cell-penetrating hyperstable antibody scFv(Ras)-An extraordinary approach to cancer therapeutics

作     者：Jina Bae Yoonyee Song 

作者机构：Chadwick InternationalYeonsu-guIncheon22002South Korea Lansing Catholic High SchoolLansingMI48912USA 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2021年第6卷第4期

页      面：343-350页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Hyperstable Single chain variable fragment antibody Cell-penetrating peptide Ras protein Cancer therapeutics 

摘      要：In the modern pharmaceutical industry,monoclonal antibodies are often used as therapeutic ***,they are restricted to cell surface antigens due to their inability to penetrate the outer cell membrane and maintain normal function in the reducing ***,it can lead to cytotoxicity since it attacks cancerous cells by mimicking the human immune *** an alternative,this study modifies the hyperstable single-chain fragment variable(scFv)antibody to eliminate cancer using its linear *** scFv(F8)antibody model was modified to recognize human Ras protein by altering residues in the antigen-binding ***,a cell-penetrating peptide(CPP)was attached to the scFv(Ras)antibody model to allow entrance to the cell,creating CPP-scFv(Ras).Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE),western blotting,and the binding assay were performed to prove its *** a result,CPP-scFv(Ras)was successfully engineered and bound to the antigen,HRas(G12V).