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radix paeoniae alba attenuates radix Bupleuri-induced hepatotoxicity by modulating gut microbiota to alleviate the inhibition of saikosaponins on glutathione synthetase

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Journal of Pharmaceutical Analysis 2023年第6期13卷 640-659页

作者： Congcong Chen Wenxia Gong Junshen Tian Xiaoxia Gao Xuemei Qin Guanhua Du Yuzhi Zhou Modern Research Center for Traditional Chinese Medicine The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of EducationShanxi UniversityTaiyuan030006China Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province Shanxi UniversityTaiyuan030006China Institute of Materia Medica Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100050China

radix Bupleuri(RB)is commonly used to treat depression,but it can also lead to hepatotoxicity after longterm *** many anti-depression prescriptions,RB is often used in combination with radix paeoniae alba(RPA)as an herb ***,whether RPA can alleviate RB-induced hepatotoxicity remain *** this work,the results confirmed that RB had a dose-dependent antidepressant effect,but the optimal antidepressant dose caused ***,RPA effectively reversed RB-induced ***,the mechanism of RB-induced hepatotoxicity was *** results showed that saikosaponin A and saikosaponin D could inhibit GSH synthase(GSS)activity in the liver,and further cause liver injury through oxidative stress and nuclear factor kappa B(NF-kB)/NOD-like receptor thermal protein domain associated protein 3(NLRP3)***,the mechanisms by which RPA attenuates RBinduced hepatotoxicity were *** results demonstrated that RPA increased the abundance of intestinal bacteria with glycosidase activity,thereby promoting the conversion of saikosaponins to saikogenins in *** from saikosaponin A and saikosaponin D,which are directly combined with GSS as an inhibitor,their deglycosylation conversion products saikogenin F and saikogenin G exhibited no GSS binding *** on this,RPA can alleviate the inhibitory effect of saikosaponins on GSS activity to reshape the liver redox balance and further reverse the RB-induced liver inflammatory response by the NFkB/NLRP3 *** conclusion,the present study suggests that promoting the conversion of saikosaponins by modulating gut microbiota to attenuate the inhibition of GSS is the potential mechanism by which RPA prevents RB-induced hepatotoxicity.

关键词： radix Bupleuri radix paeoniae alba Hepatotoxicity Gut microbiota Saikosaponins Combination mechanisms

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Rapid monitoring the water extraction process of radix paeoniae alba using near infrared spectroscopy

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Journal of Innovative Optical Health Sciences 2017年第3期10卷 69-76页

作者： Tian Hu Tongtong Li Lei Nie Lixuan Zang Hengchang Zang Yingzi Zeng School of Pharmaceutical Sciences Shandong University Wenhuaxi Road 44Jinan250012P.R.China Shandong Wohua Pharmaceutical Technology Co. Ltd Weifang261205P.R.China

Near infrared(NIR)spectroscopy has been developed into one of the most important process analytical techniques(PAT)in a wide field of *** feasibility of NIR spectroscopy with partial least square regression(PLSR)to monitor the concentration of paeoniflorin,albi-florin,gallic acid,and benzoyl paeoniforin during the water extraction process of radix paeoniae alba was demonstrated and verified in this *** spectra were collected in transmission mode and pretreated with smoothing and/or derivative,and then quantitative models were built up using *** partial least squares(iPLS)method was used for the selection of spectral *** coeficients(R2aI and R2red),root mean squares error of prediction(RMSEP),root mean squares error of calibration(RMSEC),and residual predictive deviation(RPD)were applied to verify the performance of the models,and the corresponding values were 0.9873 and 0.9855,0.0487 mg/mL,0.0545mg/mL and 8.4 for paeoniforin;0.9879,0.9888,0.0303 mg/mL,0.0321 mg/mL and 9.1 for albiflorin;0.9696,0.9644,0.0140 mg/mL,0.0145 mg/mL and 5.1 for gallic acid;0.9794,0.9781,0.00169 mg/mL,0.00171 mg/mL and 6.9 for benzoyl paeoniflorin,*** results turned out that this approach was very efficient and environmentally friendly for the quantitative monitoring of the water extraction process of radix paeoniae alba.

关键词： Near infrared spectroscopy partial least squares regression high performance liquid chromatography radix paeoniae alba

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Identification of metabolites of radix paeoniae alba extract in rat bile, plasma and urine by ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry

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Journal of Pharmaceutical Analysis 2014年第1期4卷 14-25页

作者： Zheng-Wei Chen Ling Tong Shu-Ming Li Dong-Xiang Li Ying Zhang Shui-Ping Zhou Yong-Hong Zhu He Sun Department of Pharmaceutical Analysis China Pharmaceutical University Tasly R&D Institute Tianjin Tasly Group Co. Ltd. Center of Drug Metabolism and Pharmacokinetics China Pharmaceutical University

Ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry （UPLC-Q-TOF/MS） was developed to identify the absorbed parent components and metabolites in rat bile, plasma and urine after oral administration of radix paeoniae alba extract （RPAE）. A total of 65 compounds were detected in rat bile, plasma and urine samples, including 11 parent compounds and 54 metabolites. The results indicated that glucuronidation, hydroxylation and methylation were the major metabolic pathways of the components of RPAE. Furthermore, the results of this work demonstrated that UPLC-Q-TOF/MS combined with MetaboLynx？ software and mass defect filtering （MDF） could provide unique high throughput capabilities for drug metabolism study, with excellent MS mass accuracy and enhanced MSE data acquisition. With the MSE technique, both precursor and fragment mass spectra can be simultaneously acquired by alternating between high and low collision energy during a single chromatographic run.

关键词： radix paeoniae alba Metabolite profiling UPLC-Q-TOF/MS Monoterpene glycosides

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Network pharmacology-based analysis of the mechanism of radix paeoniae alba against Toosendan Fructus-induced hepatotoxicity

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Gastroenterology & Hepatology Research 2022年第1期4卷 19-24页

作者： Heng Xu Yaqi Zhang Huan Chen Tengfei Bai School of Life Sciences Beijing University of Traditional Chinese MedicineBeijingChina100029 College of Traditional Chinese Medicine Hebei UniversityBaodingHebeiChina071000.

Objective:Objective:To analyze and explore the key targets and molecular mechanisms of action of radix paeoniae alba against Toosendan Fructus-induced hepatotoxicity and the relationship between corresponding compounds based on network ***:Using network pharmacology,a"traditional Chinese medicine-chemical composition-key target-pathway"analysis was conducted on radix paeoniae alba for the treatment of Toosendan Fructus-induced *** possible mechanism of action was analyzed in terms of ***:The core targets,such as interleukin(IL)-6,tumor necrosis factor(TNF),heat shock protein 90 alpha family class A member 1(HSP90AA1),peroxisome proliferator-activated receptor gamma(PPARG),prostaglandin-endoperoxide synthase 2(PTGS2),heme oxygenase 1(HMOX1),Jun proto-oncogene(JUN),caspase-3,estrogen receptor 1(ESR1),and aryl hydrocarbon receptor(AHR)were screened from the targets of radix paeoniae alba against Toosendan Fructus-induced *** process(BP)of toxic targets(BP terms)involved"response to drug;activation of cysteine-type endopeptidase activity involved in apoptotic process,”positive regulation of *** components(CC terms)mainly involved cytosol and membrane *** function(MF)terms included"protein homodimerization activity,"RNA polymerase II transcription factor activity and enzyme binding,etc."The Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway included the TNF signaling pathway,cancer pathways,and ***:radix paeoniae alba might alleviate Toosendan Fructus-induced hepatotoxicity through IL6,TNF,HSP90AA1,PPARG,PTGS2,HMOX1,and other targets,possibly via the activation of cysteine-type endopeptidase activity involved in these pathways.

关键词： radix paeoniae alba Toosendan Fructus liver toxicity network pharmacology compatibility

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A strategy of screening and binding analysis of bioactive components from traditional Chinese medicine based on surface plasmon resonance biosensor

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Journal of Pharmaceutical Analysis 2022年第3期12卷 500-508页

作者： Diya Lv Jin Xu Minyu Qi DongyaoWang Weiheng Xu Lei Qiu Yinghua Li Yan Cao Center for Instrumental Analysis School of PharmacySecond Military Medical UniversityShanghai200433China Department of Neurology Changzheng HospitalSecond Military Medical UniversityShanghai200003China Department of Biochemical Pharmacy School of PharmacySecond Military Medical UniversityShanghai200433China Department of Pharmaceutical Analysis School of PharmacySecond Military Medical UniversityShanghai200433China Institute of Translational Medicine Shanghai UniversityShanghai200444China

Elucidating the active components of traditional Chinese medicine(TCM)is essential for understanding the mechanisms of TCM and promote its rational use as well as TCM-derived drug *** studies have shown that surface plasmon resonance(SPR)technology is promising in this *** the present study,we propose an SPR-based integrated strategy to screen and analyze the major active components of *** used radix paeoniae alba(RPA)as an example to identify the compounds that can account for its anti-inflammatory mechanism via tumor necrosis factor receptor type 1(TNF-R1).First,RPA extraction was analyzed using an SPR-based screening system,and the potential active ingredients were collected,enriched,and identified as paeoniflorin and ***,the affinity constants of paeoniflorin and paeonol were determined as 4.9 and 11.8 mM,***,SPR-based competition assays and molecular docking were performed to show that the two compounds could compete with tumor necrosis factor-a(TNF-a)while binding to the subdomain 1 site of ***,in biological assays,the two compounds suppressed cytotoxicity and apoptosis induced by TNF-a in the L929 cell *** findings prove that SPR technology is a useful tool for determining the active ingredients of TCM at the molecular level and can be used in various aspects of drug *** SPR-based integrated strategy is reliable and feasible in TCM studies and will shed light on the elucidation of the pharmacological mechanism of TCM and facilitate its modernization.

关键词： Surface plasmon resonance Bioactive components Screening Binding analysis radix paeoniae alba

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