A strategy of screening and binding analysis of bioactive components from traditional Chinese medicine based on surface plasmon resonance biosensor
作者机构：Center for Instrumental AnalysisSchool of PharmacySecond Military Medical UniversityShanghai200433China Department of NeurologyChangzheng HospitalSecond Military Medical UniversityShanghai200003China Department of Biochemical PharmacySchool of PharmacySecond Military Medical UniversityShanghai200433China Department of Pharmaceutical AnalysisSchool of PharmacySecond Military Medical UniversityShanghai200433China Institute of Translational MedicineShanghai UniversityShanghai200444China
出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)
年 卷 期：2022年第12卷第3期
页 面：500-508页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 080202[工学-机械电子工程] 08[工学] 0802[工学-机械工程] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by grants from the National Natural Science Foundation of China(Grant Nos.:82003711 and 81703526) the Shanghai Sailing Program(Grant No.:19YF1459400)
主 题：Surface plasmon resonance Bioactive components Screening Binding analysis Radix Paeoniae Alba
摘 要：Elucidating the active components of traditional Chinese medicine(TCM)is essential for understanding the mechanisms of TCM and promote its rational use as well as TCM-derived drug *** studies have shown that surface plasmon resonance(SPR)technology is promising in this *** the present study,we propose an SPR-based integrated strategy to screen and analyze the major active components of *** used Radix Paeoniae Alba(RPA)as an example to identify the compounds that can account for its anti-inflammatory mechanism via tumor necrosis factor receptor type 1(TNF-R1).First,RPA extraction was analyzed using an SPR-based screening system,and the potential active ingredients were collected,enriched,and identified as paeoniflorin and ***,the affinity constants of paeoniflorin and paeonol were determined as 4.9 and 11.8 mM,***,SPR-based competition assays and molecular docking were performed to show that the two compounds could compete with tumor necrosis factor-a(TNF-a)while binding to the subdomain 1 site of ***,in biological assays,the two compounds suppressed cytotoxicity and apoptosis induced by TNF-a in the L929 cell *** findings prove that SPR technology is a useful tool for determining the active ingredients of TCM at the molecular level and can be used in various aspects of drug *** SPR-based integrated strategy is reliable and feasible in TCM studies and will shed light on the elucidation of the pharmacological mechanism of TCM and facilitate its modernization.
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