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rop11 gtpase Negatively Regulates ABA Signaling by Protecting ABI1 Phosphatase Activity from Inhibition by the ABA Receptor RCAR1/PYL9 in Arabidopsis

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Journal of Integrative Plant Biology 2012年第3期54卷 180-188页

作者： Zixing Li Zheng Li Xiang Gao Viswanathan ChinnusamyI Ray Bressant Zhi-Xin Wang Jian-Kang Zhu Jia-Wei Wu Dong Liu MOE Key Laboratory of Bioinformatics School of Life Sciences Tsinghua University Beijing 100084 China Division of Plant Physiology Indian Agricultural Research Institute New Delhi 110012 India Department of Horticulture and Landscape Purdue University West Lafayette Indiana 47907 USA

The phytohormone abscisic acid （ABA） regulates many key processes in plants, such as seed germina- tion, seedling growth, and abiotic stress tolerance. In recent years, a minimal set of core components of a major ABA signaling pathway has been discovered. These components include a RCAR/PYR/PYL family of ABA receptors, a group of PP2C phosphatases, and three SnRK2 kinases. However, how the interactions between the receptors and their targets are regulated by other proteins remains largely unknown. In a companion paper published in this issue, we showed that rop11, a member of the plant- specific Rho-like small gtpase family, negatively regulates multiple ABA responses in Arabidopsis. The current work demonstrated that the constitutively active rop11 （CA-rop11） can modulate the RCAR1/PYL9-mediated ABA signaling pathway based on reconstitution assays in Arabidopsis thaliana protoplasts. Furthermore, using luciferase complementation imaging, yeast two-hybrid assays, co- immunoprecipitation assays in Nicotiana benthamiana and bimolecular fluorescence complementation assays, we demonstrated that CA-rop11 directly interacts with ABI1, a signaling component downstream of RCAR1/PYL9. Finally, we provided biochemical evidence that CA-rop11 protects ABI1 phosphatase activity from inhibition by RCAR1/PYL9 and thus negatively regulates ABA signaling in plant cells. A model of how rop11 acts to negatively regulate ABA signaling is presented.

关键词： rop11 gtpase ABA signaling RCAR1/PYL9A ABI1 Arabidopsis.

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rop11 gtpase is a Negative Regulator of Multiple ABA Responses in Arabidopsis

引用

Journal of Integrative Plant Biology 2012年第3期54卷 169-179页

作者： Zixing Li Jun Kang Ning Sui Dong Liu MOE Key Laboratory of Bioinformatics School of Life Sciences Tsinghua University Beijing 100084 China

The phytohormone abscisic acid （ABA） plays crucial roles in plant development and plant responses to environmental stresses. Although ABA receptors and a minimal set of core molecular components have recently been discovered, understanding of the ABA signaling pathway is still far from complete. In this work, we characterized the function of rop11, a member of the plant-specific rop small gtpases family, in the ABA signaling process. rop11 is preferentially expressed in guard cells in all plant organs with stomata. Expression of a constitutively active rop11 （CA-rop11） suppresses ABA-mediated responses, whereas reduced expression of rop11 or expression of its dominant-negative form （DN-rop11） causes the opposite phenotypes. The affected ABA-mediated responses by rop11 include seed germination, seedling growth, stomatal closure, induction of ABA-responsive genes, as well as plant response to drought stress. Furthermore, we showed that rop11 and its closest-related family member, rop10, act in parallel in mediating these responses. ABA treatment does not affect rop11 transcription and protein abundance; however, it causes the accumulation of CA-rop11 in the nucleus. These results demonstrated that rop11 is a negative regulator of multiple ABA responses in Arabidopsis.

关键词： rop11 gtpase ABA responses negative regulator rop10rop1 double mutant Arabidopsis.

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