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Beclin-1/LC3-II dependent macroautophagy was uninfluenced in ischemia-challenged vascular endothelial cells

Genes & Diseases 2022年第2期9卷 549-561页

作者： Yaping Ma Chaofan Li Yan He Tiwei Fu Li Song Qingsong Ye Fugui Zhang Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing 401147PR China Department of Oral and Maxillofacial Surgery Stomatological Hospital of Chongqing Medical UniversityChongqing 401147PR China Tianyou Hospital Wuhan University of Science&TechnologyWuhanHubei 430064PR China Department of Oral and Maxillofacial Surgery Massachusetts General HospitalBostonMA 02114USA Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing 401147PR China Center for Regenerative Medicine Renmin Hospital of Wuhan UniversityWuhanHubei 430060PR China

Autophagy has been extensively studied and occurs in many biological ***,a question remains as to whether ischemia enhances Beclin-1/LC3-II-dependent macroautophagy in vascular endothelial cells,as has been previously ***,the effect of the level of autophagy on cell or skin flap survival still requires *** created a lethal ischemia model in human umbilical vascular endothelial cells(HUVECs),performed quantitative proteomics and bioinformatics analyses,and verified the autophagic status and effect both in vitro and in *** significantly upregulated proteins encoded by autophagy-related genes(ATGs)included ATG2A,ATG3,ATG4B,ATG5,ATG7,ATG9A,ATG12,ATG16,and *** significantly enhanced lysosomal proteins were cathepsin B,cathepsin D,lysosome-associated membrane protein 1(LAMP1),and ***,the differentially expressed proteins excluded Beclin-1,microtubule-associated protein light chain 3(LC3)-I,and *** blot analyses verified that the protein expression levels of Beclin-1,LC3-I,and LC3-II were neither upregulated nor downregulated in ischemia-challenged *** autophagic status was not enhanced by rapamycin in ischemic HUVECs but appeared to be inhibited by *** in vivo study on rats showed that a downregulation in autophagic status jeopardized skin flap *** conclusion,Ischemia neither enhanced nor inhibited Beclin-1/LC3-II-dependent canonical macroautophagy both in vitro and in vivo,in contradiction to previous *** appropriate autophagic homeostasis can minimize cell or skin flap damage.

关键词： Autophagy Bioinformatics analysis Human umbilical vein endothelial cell quantitative proteomics study Skin flap

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