Beclin-1/LC3-II dependent macroautophagy was uninfluenced in ischemia-challenged vascular endothelial cells

作     者：Yaping Ma Chaofan Li Yan He Tiwei Fu Li Song Qingsong Ye Fugui Zhang 

作者机构：Chongqing Key Laboratory of Oral Diseases and Biomedical SciencesChongqing 401147PR China Department of Oral and Maxillofacial SurgeryStomatological Hospital of Chongqing Medical UniversityChongqing 401147PR China Tianyou HospitalWuhan University of Science&TechnologyWuhanHubei 430064PR China Department of Oral and Maxillofacial SurgeryMassachusetts General HospitalBostonMA 02114USA Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher EducationChongqing 401147PR China Center for Regenerative MedicineRenmin Hospital of Wuhan UniversityWuhanHubei 430060PR China 

出 版 物：《Genes & Diseases》 (基因与疾病（英文）)

年 卷 期：2022年第9卷第2期

页      面：549-561页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 0703[理学-化学] 10[医学] 

基　　金：This work was supported by the National Natural Science Foundation of China(No.81400493) Scientific and Technological Research Program of Chongqing Municipal Education Commission(No.KJQN20200429) Joint Medical Research Project by Chongqing Health Commission and Natural Science Foundation of Chongqing(No.2020GDRC008) 

主　　题：Autophagy Bioinformatics analysis Human umbilical vein endothelial cell Quantitative proteomics study Skin flap 

摘      要：Autophagy has been extensively studied and occurs in many biological ***,a question remains as to whether ischemia enhances Beclin-1/LC3-II-dependent macroautophagy in vascular endothelial cells,as has been previously ***,the effect of the level of autophagy on cell or skin flap survival still requires *** created a lethal ischemia model in human umbilical vascular endothelial cells(HUVECs),performed quantitative proteomics and bioinformatics analyses,and verified the autophagic status and effect both in vitro and in *** significantly upregulated proteins encoded by autophagy-related genes(ATGs)included ATG2A,ATG3,ATG4B,ATG5,ATG7,ATG9A,ATG12,ATG16,and *** significantly enhanced lysosomal proteins were cathepsin B,cathepsin D,lysosome-associated membrane protein 1(LAMP1),and ***,the differentially expressed proteins excluded Beclin-1,microtubule-associated protein light chain 3(LC3)-I,and *** blot analyses verified that the protein expression levels of Beclin-1,LC3-I,and LC3-II were neither upregulated nor downregulated in ischemia-challenged *** autophagic status was not enhanced by rapamycin in ischemic HUVECs but appeared to be inhibited by *** in vivo study on rats showed that a downregulation in autophagic status jeopardized skin flap *** conclusion,Ischemia neither enhanced nor inhibited Beclin-1/LC3-II-dependent canonical macroautophagy both in vitro and in vivo,in contradiction to previous *** appropriate autophagic homeostasis can minimize cell or skin flap damage.