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purity identification of Xinkui 19 by SSR Marker Technique

Agricultural Biotechnology 2016年第1期5卷 5-7页

作者： Yating WANG Wei DUAN Yanze GONG Northeast Forestry University Harbin 150040China Xinjiang Kangdi Seed Science&Technology Co. Ltd.Urumqi 830011China

In this study, by using Xinkui 19 and its parents as experimental materials, 100 pairs of SSR molecular markers were screened to obtain specific poly- morphic primers, aiming at providing an accurate and efficient method for identifying the purity of Helianthus annuus L. hybrid seeds. According to the experimen- tal results, by using polymorphic SSR primer 455, the amplified bands of female and male parents were 460 and 430 bp, respectively; by using polymorphic SSR primer 478, the amplified bands of female and male parents were 330 and 350 bp, respectively. The identification results of Xinkui 19 hybrids and its parents with SSR marker technique and field cultivation were basically consistent. SSR primers 455 and 478 could be used for rapid and effective identification of the purity of Xinkui 19 hybrid seeds.

关键词： SSR marker Xinkui 19 purity identification

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A Simple SSR-based Method for purity identification of Cucurbita moschata Hybrids

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Agricultural Biotechnology 2014年第5期3卷 34-37,41页

作者： Xiaoxia HAN Jianwen SU Xinjun HU Zuhua YUAN Yongqi LI Jianguo YANG Vegetable Engineering Technology Research Center of Hunan Province/Hunan Vegetable Research Institute

Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR, such as complex operation and difficult application in production practice, in this study, a simple SSR-based method was established for purity identification of Cucurbita moschata hybrids. By using the established simple method, without grinding, freezing, centrifugation and drying, the genomic DNA extraction process is shorter than 3 min. Compared with the conventional method, PCR detection system and silver staining in polyacrylamide gel electrophoresis of the established method are more time-saving and cost-saving. The whole detection process is shorter than 4 h, and 480 samples can be detected with this method by one person in one day. In addition, the detection result exhibits a coincidence rate of 99% with field identification. The simple SSR-based method established in this study can provide basis for large-scale rapid purity identification of Cucurbita moschata hybrids.

关键词： Cucurbita moschata Hybrids SSR purity identification

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Screening of SSR Core Primers for purity identification of Pepper(Capsicum) Hybrids

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Agricultural Science & Technology 2015年第10期16卷 2155-2158,2230页

作者：管俊娇黄清梅张鹏马芙蓉张惠张建华云南省农业科学院质量标准与检测技术研究所云南昆明650205

[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids （accounting for 97%） had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.

关键词： Pepper SSR Hybrid purity identification Core primers

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