Purity Identification of Xinkui 19 by SSR Marker Technique
Purity Identification of Xinkui 19 by SSR Marker Technique作者机构：Northeast Forestry UniversityHarbin 150040China Xinjiang Kangdi Seed Science&Technology Co.Ltd.Urumqi 830011China
出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)
年 卷 期：2016年第5卷第1期
页 面：5-7页
基 金：Supported by Project of Xinjiang Production and Construction Corps to Support Xinjiang Development(2014AB007) Key Scientific and Technological Project of Xinjiang Production and Construction Corps(2014BA005)
主 题：SSR marker Xinkui 19 Purity identification
摘 要：In this study, by using Xinkui 19 and its parents as experimental materials, 100 pairs of SSR molecular markers were screened to obtain specific poly- morphic primers, aiming at providing an accurate and efficient method for identifying the purity of Helianthus annuus L. hybrid seeds. According to the experimen- tal results, by using polymorphic SSR primer 455, the amplified bands of female and male parents were 460 and 430 bp, respectively; by using polymorphic SSR primer 478, the amplified bands of female and male parents were 330 and 350 bp, respectively. The identification results of Xinkui 19 hybrids and its parents with SSR marker technique and field cultivation were basically consistent. SSR primers 455 and 478 could be used for rapid and effective identification of the purity of Xinkui 19 hybrid seeds.
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