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The growth of the screw dislocation of nacreous layer on pteria penguin

Science China Earth Sciences 2011年第7期54卷 951-958页

作者： QI LiJian HUANG YiLan ZHOU ZuYi ZHOU ZhengYu State Key Laboratory of Marine Geology Tongji University Shanghai 200092 China Faculty of Earth Sciences China University of Geosciences Wuhan 430074 China

In response to various environmental factors in offshore South China Sea,a group of microstructures of the screw dislocation are assembled in multiphase screw dislocations on the surface of the nacreous layer of pteria penguin from the coastal waters of Hainan Island,*** results of the transmission and scanning electronic microscope testing show that organic matter periodically secreted by epithelial histology of mantle of pteria penguin pre-forms the original screw dislocation growth tem-plate in the form of film,along with its function to select,identify,and adhere to the amorphous calcium carbonate particles as the fine organic venations diffused in the epitaxial screw growth *** the inducement and screw modulation of the or-ganic film and venations,the amorphous calcium carbonate particles gradually evolve into the pseudohexagonal *** microsheet with long-range order and screw dislocation structure by location-selecting tropism and screw dislocation *** micron-scale aragonite microsheets join in the cluster's interactive movement and screw dislocation self-assembly of the organic film,stacking forward along the axis C spirally and forming the nacreous layer with screw dislocation structure.

关键词： pteria penguin nacreous layer organic film aragonite micro sheet screw dislocation

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Determination of Cd-MT in pteria penguin with Indirect Non-Competitive ELISA

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Wuhan University Journal of Natural Sciences 2011年第4期16卷 349-356页

作者： WU Xiaoping LIAO Ailin ZHANG Chaohua LIAO Yan LU Hongyu YANG Jie College of Food Science and Technology Guangdong Ocean University Zhanjiang 524088 Guangdong China Modem Biochemistry Center Guangdong Ocean University Zhanjiang 524088 Guangdong China

Cadmium-metallothionein （Cd-MT） of pteria penguin was used to immunize domestic rabbit in order to obtain polyclonal antibody against Cd-MT. Using the anti-Cd-MT antibody, a method of indirect non-competitive enzyme-linked immunosorbent assay （non-competitive ELISA） was established to detect shellfish Cd-MT. In this case, the minimum detectable concentration of P. penguin Cd-MT was （4.563±0.051） ng/mL. The linear range of detection was 9.75-2.0×104 ng/mL. Intra-assay relative standard deviation （RSD） was less than 11.6%, and inter-assay RSD less than 6.7%. The recovery rates ranged from 83.7% to 119.0%. The polyclonal antibody against Cd-MT of P. penguin can also be used to detect MT of other four types of shellfish. In the crude extracts from different organs of P. penguin, the MT concentrations determined by this method matched well with the concentrations of cadmium detected by the atomic absorption spectroscopy.

关键词： pteria penguin Cd-MT ELISA(enzyme-linked immunosorbent assay)

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