Determination of Cd-MT in Pteria penguin with Indirect Non-Competitive ELISA

作     者：WU Xiaoping LIAO Ailin ZHANG Chaohua LIAO Yan LU Hongyu YANG Jie 

作者机构：College of Food Science and Technology Guangdong Ocean University Zhanjiang 524088 Guangdong China Modem Biochemistry Center Guangdong Ocean University Zhanjiang 524088 Guangdong China 

出 版 物：《Wuhan University Journal of Natural Sciences》 (武汉大学学报（自然科学英文版）)

年 卷 期：2011年第16卷第4期

页      面：349-356页

学科分类：0908[农学-水产] 09[农学] 0903[农学-农业资源与环境] 

基　　金：Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System (CARS-48-07B) Guangdong Provincial Agriculture Key Project (2010B020313005) 

主　　题：Pteria penguin Cd-MT ELISA(enzyme-linked immunosorbent assay) 

摘      要：Cadmium-metallothionein （Cd-MT） of Pteria penguin was used to immunize domestic rabbit in order to obtain polyclonal antibody against Cd-MT. Using the anti-Cd-MT antibody, a method of indirect non-competitive enzyme-linked immunosorbent assay （non-competitive ELISA） was established to detect shellfish Cd-MT. In this case, the minimum detectable concentration of P. penguin Cd-MT was （4.563±0.051） ng/mL. The linear range of detection was 9.75-2.0×104 ng/mL. Intra-assay relative standard deviation （RSD） was less than 11.6%, and inter-assay RSD less than 6.7%. The recovery rates ranged from 83.7% to 119.0%. The polyclonal antibody against Cd-MT of P. penguin can also be used to detect MT of other four types of shellfish. In the crude extracts from different organs of P. penguin, the MT concentrations determined by this method matched well with the concentrations of cadmium detected by the atomic absorption spectroscopy.