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Inhibitory effect of ginsenoside Rb1 on calcineurin signal pathway in cardiomyocyte hypertrophy induced by prostaglandin f2α

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Acta Pharmacologica Sinica 2007年第8期28卷 1149-1154页

作者： Qing-song JIANG~2 Xie-nan HUANG Gui-zhong YANG~4 Xiao-yan JIANG~5 Qi-xin ZHOU~(2,6) ~2Department of Pharmacology,Chongqing Medical University,Chongqing 400016,China ~3Department of Pharmacology,Zunyi Medical College,Zunyi 563003,China ~4Department of Biochemistry Zunyi Medical College,Zunyi 563003,China ~5Chengdu Vocational College of Agricultural Science and Technology,Chengdu 611100,China Department of Pharmacology Chongqing Medical UniversityChongqing400016China Department of Pharmacology Zunyi Medical CollegeZunyi 563003China Department ofBiochemistry Zunyi Medical CollegeZunyi 563003China

Aim:To examine the antihypertrophic effect of ginsenoside Rb1（Rb1）induced byprostaglandin f2α(PGf2α)in vitro and to investigate the possible mechanismsinvolved in the calcineurin（CAN）signal transduction ***:Thecardiomyocyte hypertrophy induced by PGf2αand the antihypertrophic effect ofRbiwere evaluated in primary culture by measuring the cell diameter,proteincontent,and atrial natriuretic peptide（ANP）mRNA *** and CaNmRNA expressions,CaN and its downstream effectors NfAT3and GATA4 proteinexpressions,and the intracellular free Ca2+concentration([Ca2+]i)were assayedby RT-PCR,Western blot,and fluorescent determination using fura 2/AM,***:PGf2α（100 nmol/L）significantly increased the cardiomyo-cyte diameter,protein content and[Ca2+]i,and promoted ANP,CaN mRNA,andCaN/NfAT3/GATA4protein expressions,which were inhibited by either Rb1in aconcentration-dependent manner（50,100,and 200μg/mL）or L-arginine（1 mmol/L）.NG-nitro-L-arginine-methyl ester,a nitric oxide synthase inhibitor,could abolishthe effects of L-arginine,but failed to change the effects of Rb1in the ***:The present data implicate that Rb1attenuates cardiachypertrophy,the underlying mechanism may be involved in the inhibition of theCa2+-CaN signal transduction pathway.

关键词： Rb1 prostaglandin f2α cardiac hypertrophy calcineurin

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Synchronization of ovulation in goats using prostaglandin f2α based protocols during the breeding season

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Journal of Coastal Life Medicine 2016年第3期4卷 240-243页

作者： João Simões Department of Veterinary Science University of Trás-os-Montes e Alto Douro5000-811Vila RealPortugal

The main aim of this review was to describe the feasibility of prostaglandin f2α(PGf2α)based protocols used as a tool for ovulation synchronization in cycling *** is a reproductive seasonality in small ***,from latitudes 45°towards equator,the intensity of anoestrus progressively decreases and tends to disappear in local ***,PGf2α or their synthetic analogues as luteolytic substances can assume a great importance in reproductive management of flocks from these ***,a single or double(9–11 days apart)PGf2α administration provokes a good induction but a moderate synchronization of ovulations if timed artificial insemination is considered,and a significant short oestrous cycle can occur with detrimental effects on fertility rate when compared with conventional progesterone-based *** order to minimize this constraint,some gonadotropinreleasing hormone-PGf2α-gonadotropin-releasing hormone(Ovsynch)-based protocols and their modifications,manipulating the dominant follicles and corpora lutea,were successfully tested in *** to cows,these PGf2αbased protocols seem to be a promising and more cost-effective tool for reproductive management in cycling goats.

关键词： Artificial insemination Gonadotropin-releasing hormone Goats Ovarian Ovulation prostaglandin f2α Reproduction

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Effects of prostaglandin f_(2α) on small intestinal interstitial cells of Cajal

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World Journal of Gastroenterology 2011年第9期17卷 1143-1151页

作者： Chan Guk Park Young Dae Kim Man Yoo Kim Jae Woong Koh Jae Yeoul Jun Cheol Ho Yeum Insuk So Seok Choi Department of Internal Medicine College of MedicineChosun University Department of Ophthalmology College of MedicineChosun University Department of Physiology College of MedicineChosun University Department of Physiology and Biophysics College of MedicineSeoul National University

AIM：To explore the role of prostaglandin f2α（PGf2α） on pacemaker activity in interstitial cells of Cajal（ICC）from mouse small intestine. METHODS：In this study,effects of PGf2αin the cultured ICC cells were investigated with patch clamp technology combined with Ca 2＋ image analysis. RESULTS：Externally applied PGf2α（10μmol/L）produced membrane depolarization in current-clamp mode and increased tonic inward pacemaker currents in voltage-clamp *** application of flufenamic acid（a non-selective cation channel inhibitor）or niflumic acid（aCl channel inhibitor）abolished the generation of pacemaker currents but only flufenamic acid inhibited the PGf2α-induced tonic inward *** addition,the tonic inward currents induced by PGf2αwere not inhibited by intracellular application of 5’-[-thio]diphosphate trilithium *** with Ca 2＋ free solution, U-73122,an active phospholipase C inhibitor,and thapsigargin,a Ca 2＋ -ATPase inhibitor in endoplasmic reticulum,abolished the generation of pacemaker currents and suppressed the PGf2α-induced tonic inward ***,chelerythrine or calphostin C,protein kinase C inhibitors,did not block the PGf2α-induced effects on pacemaker *** recording intracellular Ca 2＋（[Ca 2＋ ]i）concentration using fluo-3/AM,PGf2α broadly increased the spontaneous[Ca 2＋ ]i oscillations. CONCLUSION：These results suggest that PGf2αcan modulate pacemaker activity of ICC by acting non-selective action channels through phospholipase C-dependent pathway via[Ca2＋]i regulation

关键词： prostaglandin f2α Interstitial cells of Cajal Tonic inward currents Intestinal motility

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Pregnancy recognition signaling mechanisms in ruminants and pigs

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Journal of Animal Science and Biotechnology 2013年第3期4卷 170-179页

作者： fuller W Bazer Department of Animal Science Texas A&M University College Station 442D Kleberg Center2471 TAMU Texas 77843-2471 USA

Maternal recognition of pregnancy refers to the requirement for the conceptus（embryo and its associated extraembryonic membranes） to produce a hormone that acts on the uterus and/or corpus luteum（CL） to ensure maintenance of a functional CL for production of progesterone;the hormone required for pregnancy in most *** pregnancy recognition signal in primates is chorionic gonadotrophin which acts directly on the CL via luteinizing hormone receptors to ensure maintenance of functional CL during *** ruminants,interferon tau（IfNT） is the pregnancy recognition *** is secreted during the peri-implantation period of pregnancy and acts on uterine epithelia to silence expression of estrogen receptor alpha and oxytocin receptor which abrogates the oxytocin-dependent release of luteolytic pulses of prostaglandin f2-alpha（PGf） by uterine epithelia;therefore,the CL continues to produce progesterone required for *** conceptuses secrete interferon delta and interferon gamma during the peri-implantation period of pregnancy,but there is no evidence that they are involved in pregnancy recognition ***,pig conceptuses secrete abundant amounts of estrogens between Days 11 to 15 of pregnancy required for maternal recognition of ***,likely in concert with prolactin,prevents secretion of PGf into the uterine venous drainage（endocrine secretion）,but maintains secretion of PGf into the uterine lumen（exocrine secretion） where it is metabolized to a form that is not *** PGf is sequestered within the uterine lumen and unavailable to induce luteolysis,functional CL are maintained for production of *** addition to effects of chorionic gonadotrophin,IfNT and estrogens to signal pregnancy recognition,these hormones act on uterine epithelia to enhance expression of genes critical for growth and development of the conceptus.

关键词： Pregnancy Interferon Tau Estrogen prostaglandin f2α

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