检索结果-南通市图书馆

promoter methylation status of hMLH1,MGMT,and CDKN2A/p16 in colorectal adenomas

World Journal of Gastroenterology 2010年第28期16卷 3553-3560页

作者： Vasiliki Psofaki Chryssoula Kalogera Nikolaos Tzambouras Dimitrios Stephanou Epameinondas Tsianos Konstantin Seferiadis Georgios Kolios Laboratory of Clinical Chemistry University of Ioannina Medical School45500 IoanninaGreece Laboratory of Biochemistry University Hospital of IoanninaLeoforos S.Niarchou45500 IoanninaGreece Hepatogastroenterology Unit of Internal Medicine University Hospital of IoanninaLeoforos S.Niarchou45500 IoanninaGreece Laboratory of Pathology University Hospital of IoanninaLeoforos S.Niarchou45500 IoanninaGreece

AIM:To investigate aberrant DNA methylation of CpG islands and subsequent low-or high-level DNA microsatellite instability(MSI)which is assumed to drive colon carcinogenesis. METHODS:DNA of healthy individuals,adenoma(tu-bular or villous/tubulovillous)patients,and colorectal carcinoma patients who underwent colonoscopy was used for assessing the prevalence of aberrant DNA methylation of human DNA mismatch repair gene mutator L homologue 1(hMLH1),Cyclin-dependent kinase inhibitor 2A(CDKN2A/p16),and O-6-methylguanine DNA methyltransferase(MGMT),as well as their rela- tion to MSI. RESULTS:The frequency of promoter methylation for each locus increased in the sequence healthy tissue/adenoma/*** showed the highest frequency in each *** and CDKN2A/p16 presented a statistically significant increase in promoter methylation between the less and more tumorigenic forms of colorectal adenomas(tubular vs tubullovillous and villous adenomas).All patients with tubulovillous/villous adenomas,as well as all colorectal cancer patients,showed promoter methylation in at least one of the examined *** findings suggest a potentially crucial role for methylation in the polyp/adenoma to cancer progres- sion in colorectal *** and methylation seem to be interdependent,as simultaneous hMLH1, CDKN2A/p16,and MGMT promoter methylation was present in 8/9 colorectal cancer patients showing the MSI phenotype. CONCLUSION:methylation analysis of hMLH1,CD- KN2A/p16,and MGMT revealed specific methylation profiles for tubular adenomas,tubulovillous/villous adenomas,and colorectal cancers,supporting the use of these alterations in assessment of colorectal tumorigenesis.

关键词： promoter methylation Microsatellite instability Human DNA mismatch repair gene mutator L homologue 1 O-6-methylguanine DNA methyltransferase Cyclin-dependent kinase inhibitor 2A

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

promoter methylation and mRNA expression of DKK-3 and WIF-1 in hepatocellular carcinoma

引用

World Journal of Gastroenterology 2009年第21期15卷 2595-2601页

作者： Zhen Ding Ye-Ben Qian Li-Xin Zhu Qi-Ru Xiong Department of Surgery First Affiliated Hospital of Anhui Medical University 218 Jixi avenue Hefei 230022 Anhui Province. China

AIM:To investigate the promoter methylation status and mRNA expression of DKK-3 and WIF-1 gene in hepatocellular carcinoma(HCC).METHODS:DKK-3 and WIF-1 acted as Wnt-antagonists and tumor suppressors,but hypermethylation of the gene promoter and low mRNA expression activated Wnt signaling aberrantly and induced the development of *** status of the DKK-3 and WIF-1 gene promoter was investigated using methylation specific polymerase chain reaction(PCR) in tumor and adjacent non-cancerous tissues from 33 HCC patients and 20 normal liver tissues served as *** expression of DKK-3 and WIF-1 mRNA was also determined by real-time quantitative reverse transcriptase *** relationship between methylation,mRNA expression,and clinical data,as well as methylation and mRNA expression of the two genes were ***:The methylation of DKK-3 and WIF-1 genes in HCC increased significantly compared with adjacent non-cancerous tissues and normal control tissues(χ2 =7.79,P methylation between adjacent non-cancerous tissues and normal control tissues was *** HCC tissues,significant differences in the DKK-3 promoter methylation were observed in age and cirrhosis,and significant differences of the WIF-1 promoter methylation were observed in HBsAg and *** average expression of DKK-3 mRNA in HCC and adjacent non-cancerous tissues was increased significantly compared with normal control *** average expression of WIF-1 mRNA showed no significant difference among the three *** mRNA expression of DKK-3 gene in HCC was decreased as the pathological grade ***:The aberrant promoter methylation and decreased expression of DKK-3 and WIF-1 may be an important mechanism in HCC,and may be a far-reaching significance in early diagnosis and therapy of HCC.

关键词： Hepatocellular carcinoma DKK-3 WIF-1 promoter methylation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Study on RIZ1 gene promoter methylation status in human esophageal squamous cell carcinoma

引用

World Journal of Gastroenterology 2012年第6期18卷 576-582页

作者： Shang-Wen Dong Peng Zhang Yi-Mei Liu Yuan-Tao Cui Shuo Wang Shao-Jie Liang Zhun He Pei Sun Yuan-Guo Wang Department of Cardiothoracic Surgery Tianjin Medical University General HospitalTianjin 300052China Tianjin Institute of Endocrinology Tianjin Medical UniversityTianjin 300070China

AIM： To investigate the promoter region methylation status of retinoblastoma protein-interacting zinc finger gene 1 （RIZ1） in the human esophageal squamous cell carcinoma （ESCC） cell lines and tissues and verify the relationship between methylation of RIZ1 and oncogen- esis, tumor progression and metastasis etc of ESCC. METHODS： methylation-specific polymerase chain reac- tion （MSP） was used to investigate the promoter region methylation status of RIZ1 in 6 ESCC cell lines. One cell line where RIZ1 promoter region methylation was de- tected was selected for the next study, where the cell line was treated with 5-aza-CdR. Real-time polymerase chain reaction was used to investigate its influence on the transcription of RIZ1. Experiments using frozenpathological specimens from 47 ESCC patients were performed using the same MSP methodology. RESULTS： promoter methylation of RIZ1 gene was detected in TEl3, CaEs17 and EC109 cell lines and the cell line TEl3 was chosen for further study. The expression of RIZl mRNA in TE-13 was up-regulated after treatment with 5-aza-CdR. The rate of methyla- tion in carcinomas tissues was significantly higher than those in matched neighboring normal and distal ending normal tissue, and the deviation of data was statisti- cally significant （2,2 = 24.136, P 〈 0.01）. Analysis of the gender, age familial history, tumour deviation, tumour saturation, lymph gland displacement and clinical stag- ing of 47 samples from ESCC patients showed that the fluctuation of data was not statistically significant. CONCLUSION： promoter methylation may play an im- portant role in the epigenetic silencing of RIZ1 gene expression in human ESCC. RIZ1 is considered to be a potential tumor suppressor gene and may be a biologi- cal parameter for testing early stage human ESCC.

关键词： Retinoblastoma protein-interacting zinc fingergene 1 Tumor suppressor genes Esophageal squamouscell carcinoma promoter methylation methylation-spe-cific polymerase chain reaction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Methyltransferase 3A-mediated promoter methylation represses retinoic acid receptor responder 3 expression in basal-like breast cancer

引用

BIOCELL 2023年第2期47卷 319-328页

作者： YOULIN TUO XUBAO LIU Department of Pancreatic Surgery West China HospitalSichuan UniversityChengdu610041China

Retinoic acid receptor responder 3(RARRES3)has been characterized as a tumor suppressor in multiple types of *** study aimed to examine the expression profile of RARRES3 across the PAM50 subtypes of breast *** DNA methylation status of RARRES3 was checked in the basal-like subtype,and the underlying mechanisms of its dysregulation were ***-sequencing(seq)and methylation data from The Cancer Genome Atlas were used for in-silico ***-like representative SUM149 and MDA-MB-468 cell lines were used for in vitro and in vivo *** to tumor-adjacent normal tissues,only the basal-like tumor tissues had significantly downregulated RARRES3 *** methylation level of four CpG sites in the promoter region showed a strong negative correlation with RARRES3 *** gene coding for DNA methyltransferase 3A(DNMT3A)had consistent positive correlations with the methylation of the CpG *** Immunoprecipitation-quantitative polymerase chain-reaction and bisulfite sequencing PCR showed that DNMT3A could bind to the promoter region of RARRES3 and promote methylation of the CpG sites within the ***3A knockdown significantly restored RARRES3 expression at the mRNA and protein level in the two cell ***-8,colony formation,and flow cytometric analysis showed that RARRES3 overexpression attenuated the growth-promoting effects of DNMT3A overexpression and also weakened the DNMT3A overexpression-induced activation of ERK1/2 and PI3K/AKT *** summary,this study revealed that DNMT3A enhances promoter methylation of the RARRES3 gene and suppresses its transcription in basal-like breast *** DNMT3A-RARRES3 signaling pathway might be a potential target for the treatment of this tumor subtype.

关键词： DNMT3A Basal-like breast cancer RARRES3 promoter methylation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cysteine dioxygenase type 1 (CDO1): Its functional role in physiological and pathophysiological processes

引用

Genes & Diseases 2023年第3期10卷 877-890页

作者： Min Chen Jie-Ying Zhu Wang-Jing Mu Liang Guo School of Kinesiology Shanghai University of SportShanghai 200438China Shanghai Frontiers Science Research Base of Exercise and Metabolic Health Shanghai University of SportShanghai 200438China

Cysteine dioxygenase type 1 (CDO1), belonging to the mammalian non-heme Fe(II) dioxygenases family, is a key enzyme for cysteine catabolism. Its activity and expression is regulated through multiple mechanisms. CDO1 is involved in a spectrum of physiological processes including lipid metabolism, adipogenesis, osteoblastic differentiation, redox homeostasis, fertility, bile acid metabolism, sulfide metabolism, and organismal growth and development. Many of these processes are regulated directly or indirectly by CDO1-mediated metabolism of cysteine. In pathophysiological processes, the degree of CDO1 promoter methylation is closely related to the progression and malignancy of tumors, and overexpression of CDO1 will promote ferroptosis of cancer cells. Moreover, CDO1 may ameliorate metabolic disorders through the taurine-mediated improvement of lipid metabolism and insulin sensitivity and improve neurodegenerative diseases by regulating cysteine level. Therefore, elucidation of the mechanisms underlying the role of CDO1 would provide a clearer view of the therapeutic potential and possible risks of targeting this important enzyme.

关键词： Anti-tumor Antioxidation Cysteine promoter methylation Taurine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dual Role of Wnt5a in the Progression of Inflammatory Diseases

引用

Chinese Medical Sciences Journal 2022年第3期37卷 265-274,I0013页

作者： Xu Chen Hongling Liu Dehong Li Jinsui Wang Fenghui Zhao Department of Pathology Gansu Provincial HospitalLazhou 730000China Department of Occupational Medicine the Third Gansu Provincial HospitalLanzhou 730000China Department of Clinical Laboratory Gansu Provincial HospitalLanzhou 730000China

Wnt5a is a secreted Wnt ligand that plays a critical role in cellular pathways and inflammatory *** WNT5A gene encodes two protein isoforms,Wnt5a-long and Wnt5a-short,which differ based on different promoter methylation and have distinct ***,the mechanisms of the promoter methylation are *** on the extent of promoter methylation,Wnt5a exerts both anti-inflammatory and proinflammatory effects in inflammatory diseases,which may be involved in different Wnt5a ***,the Wnt5a isoforms may be potential diagnostic markers for inflammatory diseases and the mechanisms of the WNT5A gene promoter methylation need to be further investigated.

关键词： Wnt5a inflammatory diseases promoter methylation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

NDRG4, a candidate tumor suppressor gene, is a potential prognostic indicator of gastric cancer

NDRG4, a candidate tumor suppressor gene, is a potential pro...

引用

2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛

作者： Lian-Lian Hong Jiu-Li Wang Xiang-Liu Chen Zhi-Qiang Ling Zhejiang Cancer Hospital

N-Myc downstream-regulated gene 4(NDRG4), a newly found candidate tumor suppressor gene, plays an important inhibitory role in multiple types of cancers. However, its function and the underlying mechanism in carcinogenesis and progression of gastric cancer(GC) have not yet to be reported. Using realtime RT-PCR and immunohistochemistry analysis, we determined that NDRG4 was significantly downregulated both in mRNA and protein levels in GC tissues, compared with that in their corresponding paracancerous histological normal tissues(PCHNT)(both Pmethylation frequencies of NDRG4 promoter in GC tissues were obviously higher than that in PCHNT(Ppromoter hypermethylation of NDRG4 was also notably correlated with various clinic-pathological parameters, including the depth of tumor invasion, vein invasion, TNM stage, lymph node metastasis, distant metastasis, H. pylori infection, etc(all Pmethylation as biomarker for GC prognosis. The Kaplan-Meir analysis showed that NDRG4 promoter methylation was associated with worse prognosis of GC patients(Ppromoter methylation is a frequent event in GC progression and an independent prognostic factor for the disease-free survival(DFS). Collectively, NDRG4 is markedly downregulated in human GC. NDRG4 expression level is closely associated with the progression and metastasis o

关键词： N-Myc downstream-regulated gene 4 (NDRG4) promoter methylation progression Wnt/β-catenin signaling gastric cancer

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Clinical Significance of Expression of Hint1 and Potential Epigenetic Mechanism in Gastric Cancer

Clinical Significance of Expression of Hint1 and Potential E...

引用

江苏省遗传学会第八届会员代表大会暨学术研讨会

作者： Huaying Huang~(12),Xianwei Su~2,Fengchang Qiao~2,Hong Fan~2,Jinfei Chen~1 (1.Department of Oncology,First Hospital Affiliated Nanjing Medical University,Nanjing,China 210006 2.Key Laboratory of Developmental genes and Human diseases,Ministry of Education,Southeast University,Nanjing,China 210009 )

＜正＞As the fourth most common cancer and the second cause of death worldwide,gastric cancer（GC） causes nearly 1000,000 new cases and over 850,000 deaths each *** infection of Helicobacter pylori（H. pylori） is a common event and is shown to induce aberrant methylation in gastric ***-Barr（EB） virus infection is considered as another important carcinogen in gastric ***1,a member of the evolutionary highly conserved HIT protein super family,is ubiquitously expressed in diverse species including mammalian *** previous studies,Hint1 deleted mice had a marked increase in susceptibility to chemical carcinogen induced gastric tumors,mammary tumors and ovarian *** evidences show that HINT1 is a haploinsufficient tumor *** the present study,we explored possible correlations between the expression level of HINT1 and clinicopathological features in tissues from GC *** mRNA expression levels of Hint1 were detected by qPCR in 34 GCs（tumor and paired adjacent non-tumor tissues specimens） and analyzed the relationship with environmental factors *** and EB *** blot was used to detect the HINT1 protein levels in 51 GCs,and IHC was used to show the immunostaining in gastric mucosal cells,respectively.

关键词： HINT1 GC H.Pylori EB virus promoter methylation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：