Promoter methylation and mRNA expression of DKK-3 and WIF-1 in hepatocellular carcinoma
Promoter methylation and mRNA expression of DKK-3 and WIF-1 in hepatocellular carcinoma作者机构：Department of Surgery First Affiliated Hospital of Anhui Medical University 218 Jixi avenue Hefei 230022 Anhui Province. China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2009年第15卷第21期
页 面：2595-2601页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 090602[农学-预防兽医学] 1002[医学-临床医学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0906[农学-兽医学]
基 金：Supported by The Natural Science Fund of Educational Department of Anhui Province No. 2006KJ094A
摘 要：AIM:To investigate the promoter methylation status and mRNA expression of DKK-3 and WIF-1 gene in hepatocellular carcinoma(HCC).METHODS:DKK-3 and WIF-1 acted as Wnt-antagonists and tumor suppressors,but hypermethylation of the gene promoter and low mRNA expression activated Wnt signaling aberrantly and induced the development of *** status of the DKK-3 and WIF-1 gene promoter was investigated using methylation specific polymerase chain reaction(PCR) in tumor and adjacent non-cancerous tissues from 33 HCC patients and 20 normal liver tissues served as *** expression of DKK-3 and WIF-1 mRNA was also determined by real-time quantitative reverse transcriptase *** relationship between methylation,mRNA expression,and clinical data,as well as methylation and mRNA expression of the two genes were ***:The methylation of DKK-3 and WIF-1 genes in HCC increased significantly compared with adjacent non-cancerous tissues and normal control tissues(χ2 =7.79,P 0.05;χ2 = 4.89,P 0.05),and no significant difference in methylation between adjacent non-cancerous tissues and normal control tissues was *** HCC tissues,significant differences in the DKK-3 promoter methylation were observed in age and cirrhosis,and significant differences of the WIF-1 promoter methylation were observed in HBsAg and *** average expression of DKK-3 mRNA in HCC and adjacent non-cancerous tissues was increased significantly compared with normal control *** average expression of WIF-1 mRNA showed no significant difference among the three *** mRNA expression of DKK-3 gene in HCC was decreased as the pathological grade ***:The aberrant promoter methylation and decreased expression of DKK-3 and WIF-1 may be an important mechanism in HCC,and may be a far-reaching significance in early diagnosis and therapy of HCC.
维普期刊数据库