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proliferating cell nuclear antigen of Ulva prolifera is involved in the response to temperature stress

Journal of Oceanology and Limnology 2024年第4期42卷 1227-1241页

作者： Hongyan HE Juanjuan YANG Yuan HE Xiuwen YANG Caiwei FU Dongren ZHANG Jingwei DONG Aurang ZEB Jing QU Songdong SHEN School of Biology&Basic Medical Sciences Soochow UniversitySuzhou 215101China Department of Pathology Affiliated Hospital and Medical School of Nantong UniversityNantong 226001China

Ulva prolifera is the most common specie causative to green tide,and its growth is sensitive to temperature ***,the mechanisms of *** response to temperature stress remain *** this study,high temperature(36℃)stimulus promoted the death of unformed cell wall protoplasts and delayed the division of formed cell wall protoplasts,while low-temperature(4℃)stimulus did not,suggesting that the mechanisms of the response of *** to high and low temperature stresses are *** results show that proliferation-related genes were differentially expressed under high and low-temperature stresses,especially the proliferating cell nuclear antigen(PCNA)and cyclins(CYCs).Subsequently,the interaction between PCNA and Cyclin A was confirmed by Co-immunoprecipitation,yeast two-hybrid,and so ***,high-and low temperature stresses induced the expression of PCNA and Cyclin A in varying of degrees,and activated extracellular signal-regulated kinase(ERK)signal *** results suggest,PCNA,Cyclin A,and ERK signal pathway played important roles in the resistance of *** to temperature ***,high-temperature stress induced an increase of miR-2916 in abundance,and exhibiting reverse expression of PCNA;and PCNA was target gene of miR-2916,suggesting that miR-2916 protected *** from high-temperature stress via post-transcriptionally regulation of *** study laid a foundation for understanding the function of PCNA and Cyclin A,moreover,it has a guiding significance to explore the mechanisms of the response to temperature stress from proliferation-related genes regulatory networks in ***.

关键词： UIva prolifera proliferating cell nuclear antigen Cyclin A extracellular signal-regulated kinase microRNA temperature stress

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proliferating cell nuclear antigen involved in the repair process of ouabain-induced brain damage independent of hypertension in rats

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Chinese Medical Journal 2014年第11期127卷 2111-2116页

作者： You Beian Shen Lin Qiu Jie Liu Xiangju Zhao Shaohua Ji Xiang Wang Yan Gao Haiqing Department of Geriatrics Qilu Hospital of Shandong University Shandong Provincial Key Laboratory of Cardiovascular Proteomics Jinan Shandong 250012 China

Background Ouabain is a mammalian adrenocortical hormone that is involved in the pathogenesis of hypertension by inhibiting Na-K ATPase *** also participates in a variety of kinase-mediated signaling pathways associated with Na-K *** studies have shown that ouabain can cause cardiac remodeling independent of elevated blood pressure and that proliferating cell nuclear antigen （PCNA） plays a coordinating role for numerous proteins involved in multiple processes associated with DNA ***,we hypothesized that ouabain might play a role in the cerebral cortex through signaling pathways independent of *** PCNA might be involved in this *** Male Sprague-Dawley rats were treated with ouabain or with 0.9% nitric sodium as the control *** blood pressure was recorded *** four weeks of treatment,morphological changes in the cerebral cortex were analyzed using light and transmission electron *** expression of PCNA in the cerebral cortex was evaluated by immunohistochemistry,real time quantitative PCR,and Western *** After 4-week treatment,there was no significant difference in systolic blood pressure compared with the control group,but both structural deterioration and up-regulated expression of PCNA in the brain was induced by ouabain *** These results suggest that ouabain induces alterations in the brain structure,and this effect is independent of blood *** might be involved in the repair process of ouabain-induced brain damage.

关键词： ouabain proliferating cell nuclear antigen hypertension brain Na-K ATPase

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proliferating cell nuclear antigen clamp associated factor,a potential proto-oncogene with increased expression in malignant gastrointestinal tumors

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World Journal of Gastrointestinal Oncology 2021年第10期13卷 1425-1439页

作者： Li-Juan Liu Jian-Ming Liao Fan Zhu State Key Laboratory of Virology and Hubei Province Key Laboratory of Allergy&Immunology Department of Medical MicrobiologySchool of MedicineWuhan UniversityWuhan 430071Hubei ProvinceChina Department of Neurosurgery Renmin HospitalWuhan UniversityWuhan 430060Hubei ProvinceChina

Gastrointestinal(GI)cancers,including malignancies in the gastrointestinal tract and accessory organs of digestion,represent the leading cause of death worldwide due to the poor prognosis of most GI *** investigation into the potential molecular targets of prediction,diagnosis,prognosis,and therapy in GI cancers is urgently *** cell nuclear antigen(PCNA)clamp associated factor(PCLAF),which plays an essential role in cell proliferation,apoptosis,and cell cycle regulation by binding to PCNA,is a potential molecular target of GI cancers as it contributes to a series of malignant properties,including tumorigenesis,epithelial-mesenchymal transition,migration,and ***,PCLAF is an underlying plasma prediction target in colorectal cancer and liver *** addition to GI cancers,PCLAF is also involved in other types of cancers and autoimmune *** pivotal pathways,including the Rb/E2F pathway,NF-κB pathway,and p53-p21 cascade,are implicated in PCLAF-mediated *** also contributes to some diseases through dysregulation of the p53 pathway,WNT signal pathway,MEK/ERK pathway,and PI3K/AKT/mTOR signal *** review mainly describes in detail the role of PCLAF in physiological status and GI *** signaling pathways involved in PCLAF are also *** of the interaction of PCLAF/PCNA or the expression of PCLAF might be potential biological therapeutic strategies for GI cancers.

关键词： proliferating cell nuclear antigen proliferating cell nuclear antigen clamp associated factor Transcript variant Gastrointestinal cancers Signal pathway Biological therapeutic

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proliferating cell nuclear antigen(PCNA) IN OVARIAN CARCINOMA AND ITS RELATION TO LYMPH NODE METASTASIS AND PROGNOSIS

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Chinese Journal of Cancer Research 1998年第4期10卷 32-35页

作者：吴小华张志毅蔡树模张国玲施达仁陆洪芬 Department of Gynecological Oncology Shanghai Medical University Shanghai 200032 Department of Pathology Cancer HospitalShanghai Medical University Shanghai 200032

Objective: To investigate expression of proliferating cell nuclear antigen (PCNA) in ovarian epithelial cancer and its relation to lymph node metastasis, outcome of second look laparotomy (SLL) and prognosis Methods: Monoclonal antibody PC10 was used to stain PCNA in archival paraffin embedded tissues Results: PC10 immunostaining was performed successfully in all 74 primary and 31 intraperitoneal metastatic tumors The expression levels of PCNA were significantly increased in 31 metastatic tumors compared with their primary tumor from the same patients (7 94 vs 6 89, P=0 042) The expression levels was more elevated in bilateral than in unilateral ovarian cancer, but it was not associated with lymph node metastasis, clinical stage, histological grade and subtype In 28 patients with stage III ovarian cancer undergone SLL, the mean immunoreactive score (IRS) of PCNA of the primary tumor was significantly higher in patients with negative SLL than in those with positive SLL (7 59 vs 6 10, P =0 03) Since chemotherapy was performed following surgical debulking, negative SLL more frequently seen in patients with high PCNA expression might suggest better chemotherapeutic sensitivity due to higher proliferation fraction of tumor cell Univariate analysis of survival indicated that the overall survival was inversely associated with the level of PCNA expression, while multivariate analysis with Cox's model showed that independent prognostic factors were the residual tumor after primary debulking ( P<0 001 ) and clinical stage ( P <0 05), followed by PCNA expression( P =0 09) Conclusion: The expression of PCNA may beuseful in predicting the patients' prognosis, but is not correlated with lymph node metastasis

关键词： Ovarian neoplasm proliferating cell nuclear antigen Lymphatic metastasis Prognosis

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Cloning and characterization of proliferating cell nuclear antigen gene of Alexandrium catenella (Dinoflagellate) with respect to cell growth

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Acta Oceanologica Sinica 2010年第3期29卷 90-96页

作者： HUANG Jian LIANG Shan SUI Zhenghong MAO Yunxiang GUO Hao College of Marine Life Sciences Ocean University of China Qingdao 266003 China National Marine Environmental Monitoring Centre State Oceanic Administration Dalian 116023 China Department of Life Science Yangtze Normal University Chongqing 408000 China

Harmful algal blooms （HABs） have been affecting negatively the shellfish and aquaculture industries around the world. Though a lot of efforts have been made to disclose the changes of environmental factors involved and their effects on the HABs events, the molecular mechanism of this process remains unclear. To address this problem, proliferating cell nuclear antigen gene （pcna） was isolated and characterized from Alexandrium catenella. It showed high homology to those of other dinoflagellates （89% and 91% homology to Pfiesteria piscicid and Pyrocystis lunula, respectively）, and also 42%–43% homology to those of plant and animals. The expression level of pcna revealed by quantitative real time PCR was the lowest at the late lagging cell growth phase, increased to the highest at the late exponential phase, and then decreased at the stationary phase. Though the cell growth rate was also changing, no positive correlation between pcna expression level and cell growth rate was displayed throughout the whole cell growth stages （r 2 =0.024 6）. However, the pcna expression level had the similar trend with the change of cell growth rate throughout the whole growing process, e.g., from increasing at the earlier cell growth stage to decreasing at the following stages, though slightly lagging to the latter.

关键词： Alexandrium catenella gene growth harmful algal blooms proliferating cell nuclear antigen

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Bladder cancer therapy using combined proliferating cell nuclear antigen antisense oligonucleotides and recombinant adenovirus p53

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Chinese Medical Journal 2003年第12期116卷 1860-1863页

作者：朱朝辉邢诗安林晨张雪艳付明梁萧曾甫清鲁功成吴旻 Department of Urology Union Hospital Department of Urology Central Laboratory Union Hospital National Laboratory of Molecular Oncology

Objective To evaluate the antitumor efficacy of proliferating cell nuclear antigen antisense oligonucleotide (PCNA-ASO) in combination with recombinant adenovirus p53 (Ad-p53) against bladder cancer EJ and BIU-87 cells in vitro and in vivo. Methods cells were transfected with Ad-p53 (100 MOI), and PCNA-ASO (1.6 μmol/L) was then introduced into the cells using a cationic lipid (lipofectamine, 20 μl/ml). In vitro and in vivo antitumor effects of combining PCNA-ASO with Ad-p53 were measured using the MTT assay, flow cytometry, clone formation, and a nude mice model. Results The combination of PCNA-ASO and Ad-p53 inhibited cell viability in both the EJ (89.3%) and BIU-87 (78.6%) cell lines. The ability of the cells to form foci was also reduced by 74.8% in EJ cells and by 67.5% in BIU-87 cells (Pcells in the S phase (11.4% in EJ cells, 14.6% in BIU-87 cells) and a significant increase of cells in G1 phase (62.2% in EJ, 56.8% in BIU-87) were noted. The mean tumor volume after 7 days of treatment with PCNA-ASO or Ad-p53 in combination decreased to 47.6% or 36.4% of the initial tumor size in the two cell lines respectively. Conclusion These results indicate that combined PCNA-ASO and Ad-p53 in the treatment of bladder cancer with mutant p53 has important therapeutic potential, significantly suppressing the growth of human bladder cancer both in vitro and in vivo.

关键词： bladder cancer Ad-p53 proliferating cell nuclear antigen antisense oligonucleotide

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Evaluation of germ-cell kinetics in infertile patients with proliferating cell nuclear antigen proliferating index

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Asian Journal of Andrology 2001年第1期3卷 63-66页

作者： Li ZENG, Xiang-Tian KONG, Jin-Wei SU, Tong-Li XIA Yan-Qun NA Ying-Lu GUO Institute of Urology,Peking University, Beijing 100034, China Institute of Urology Peking University Beijing China. zengli@***

Aim: To explore the usefulness of proliferating cell nuclear antigen proliferating index (PCNA PI) in the pathologicaldiagnosis and treatment of male infertility. Methods: Testicular biopsy specimen obtained from 48 cases of male in-fertility and 2 normal controls were fixed and embedded. The sections were stained with anti-PCNA monoclonal anti-bodies or haematoxylin/eosin. proliferating index (PI), expressed as the percentage of germ-cell nuclei positivelystained with PCNA antibody, was assessed from more than 20 seminiferous tubules or 600 germ-cells. Results: Theinfertile patients were divided into 4 groups: Group 1, normal spermatogenesis (14 cases); Group 2, hypospermato-genesis (16 cases); Group 3, germinal arrest (10 cases); Group 4, Sertoli cell only syndrome (8 cases). The PCNAPI of normal control testis was 86.5 % (mean value). Group 3 had a significantly lower PCNA PI (29.8 %) than nor-mal testis; Group 1 and 2 had similar Pis (82.3% and 82.3%, respectively) as the control testis. PI of the negativecontrol (Group 4) was 0 as no germ-cells were found. Conclusion: PCNA PI is useful for assessing germ-cell ki-netics, especially for pathological diagnosis of germinal arrest which is difficult to differentiate by routine HE stainingtechnique. In germinal arrest, there is a significantly lowered PCNA PI, which is an indication of DNA synthesis dete-rioration, suggesting the use of therapies be different from those for hypospermatogenesis. (Asian J Androl 2001 Mar;3: 63-66)

关键词： male infertility testis kinetics proliferating cell nuclear antigen

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Mast cell density and the context of clinicopathological parameters and expression of p185,estrogen receptor,and proliferating cell nuclear antigen in gastric carcinoma

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World Journal of Gastroenterology 2002年第6期8卷 1005-1008页

作者： Ying-AnJiang You-YuanZhang He-ShengLuo Shou-FuXing DepartmentofGastroenterology RenmingHospitalofWuhanUniversityWuhan430060HubeiprovinceChina DepartmentofPathology CentralHospitalofHuangshiCityHuangshi435000hubeiProvinceChina DepartmentofPatholo

AIM:To investigate the relationship between the mast cell density(MCD)and the context of clinicopathological parameters and expression of p185,estrogen receptor(ER), and proliferating cell nuclear antigen(PCNA)in gastric carcinoma. METHODS:Mast cell,p185,ER,and PCNA were detected using immunohistochemical S-P labeling *** cell was counted in tissue of gastric carcinoma and regional lymph nodes respectively,and involved lymph nodes(ILN)were examined as usual. RESULTS:MCD was significantly related to both age and depth of penetration(x^2=4.688,P21/0.03 mm^2 and MCD≤21/0.03 mm^2 in 100 patients;MCD in 1-6 ILN group patients was significantly higher than that in 7-15 ILN or>15 ILN group patients(u=6.881,8.055,P21/ 0.03 mm^2 and MCD≤21/0.03 mm^2 in 100 patients. CONCLUSION:Mast cell may have effect on inhibiting invasive growth of tumor,especially in the aged patients; The number of mast cells,in certain degree,may predicate the number of involved lymph nodes,which is valuable for assessment of prognosis;MCD was related to the expression of p185,ER,and PCNA in gastric *** suggests that mast cell accumulation may inhibit the proliferation and the dissemination of the gastric carcinoma. INTRODUCTION Recently,many studies have reported on the association of mast cell with various *** several malignancies,mast cell has been found to correlate with growth,penetration and prognosis of ***,our study was undertaken to investigate the relationship between the mast cell density (MCD)and the context of clinicopathological parameters and expression of p 185,estrogen receptor(ER),and proliferating cell nuclear antigen(PCNA)in gastric carcinoma.

关键词： Aged cell Count Humans Immunohistochemistry Lymphatic Metastasis Mast cells Middle Aged Prognosis proliferating cell nuclear antigen Receptor, erbB-2 Receptors, Estrogen Stomach Neoplasms

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Clinical significance of expression of proliferating cell nuclear antigen and E-cadherin in gastric carcinoma

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World Journal of Gastroenterology 2017年第20期23卷 3721-3729页

作者： lin Hu Hong-lang li Wei-feng li Jun-min Chen Jian-tao Yang Jun-Jing Gu lin Xin Department of Gastrointestinal Surgery The Second Affiliated Hospital of Nanchang University

AIM to investigate the expression of proliferating cell nuclear antigen(p CNA)and E-cadherin in gastric carcinoma and to analyze their clinical *** A total of 146 patients were selected for this study,including 38 patients with intestinal metaplasia,42with dysplasia,and 66 with primary gastric *** addition,40 patients with normal gastric tissues were selected as *** expression of p CNA and E-cadherin was detected by *** in p CNA and the E-cadherin labeling indexes among normal gastric mucosa,intestinal metaplasia,dysplasia,and gastric carcinoma were *** with normal gastric tissues were assigned to a normal group,while gastric cancer patients were assigned to a gastric cancer *** difference in p CNA and E-cadherin expression between these two groups was *** relationship between expression of p CNA and E-cadherin and clinicopathological features was also explored in gastric cancer ***,prognosis-related factors,as well as the expression of p CNA and E-cadherin,were analyzed in patients with gastric cancer to determine the 3-year survival of these *** the difference in p CNA and the E-cadherin labeling indexes among normal gastric mucosa,intestinal metaplasia,dysplasia,and gastric carcinoma was statistically significant(p<0.05).During the transition of normal gastric mucosa to gastric cancer,the p CNA labeling index gradually increased,while the E-cadherin labeling index gradually decreased(p<0.05).the p CNA labeling index was significantly higher and the E-cadherin labeling index was significantly lower in gastric cancer than in dysplasia(p<0.05).the expression of p CNA was significantly higher in the gastric cancer group than in the normal group,but E-cadherin was weaker(p<0.05).there was a negative correlation between the expression of p CNA and E-cadherin in gastric carcinoma(r=-0.741,p=0.000).p CNA expression differed significantly between gastric

关键词： proliferating cell nuclear antigen E-cadherin Gastric cancer Gastric mucosa

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The Effect of the LysoPC-induced Endothelial cell Conditioned Medium on proliferating cell nuclear antigen Expression of the Calf Thoracic Aorta Smooth Muscle cells

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2002年第1期22卷 28-30页

作者：周洪莲姚济华余枢 Department of Integration Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan

In order to study the effect of and mechanism of lysophosphatidylcholine (LysoPC) on proliferation of the calf thoracic aorta smooth muscle cells (ASMCs), the ASMCs were used to observe the effects of LysoPC induced endothelial cell conditioned medium on the DNA content and proliferating cell nuclear antigen (PCNA) expression in the calf thoracic ASMCs by flow cytometry and Western Blot technique. It was found that LysoPC induced endothelial cell conditioned medium could significantly promote PCNA expression of the calf ASMCs, induce the converting of ASMCs from G 0 /G 1 phase to S phase of DNA synthesis, and increase the tyrosine phosphorylation protein expression. Tyrosine protein kinase inhibitor (TPKi) RG50864 could obviously inhibit proliferation of LysoPC induced ASMCs in a dose dependence manner. The results indicated that the effect of LysoPC promoting the proliferation of ASMCs is partly evoked by endothelial cell derived growth factors such as PDGF and so on.

关键词： lysophosphatidylcholine aorta, thoracic muscle, smooth,vascular proliferating cell nuclear antigen

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