Proliferating cell nuclear antigen involved in the repair process of ouabain-induced brain damage independent of hypertension in rats

作     者：You Beian Shen Lin Qiu Jie Liu Xiangju Zhao Shaohua Ji Xiang Wang Yan Gao Haiqing 

作者机构：Department of Geriatrics Qilu Hospital of Shandong University Shandong Provincial Key Laboratory of Cardiovascular Proteomics Jinan Shandong 250012 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2014年第127卷第11期

页      面：2111-2116页

核心收录：

学科分类：081504[工学-水利水电工程] 0905[农学-畜牧学] 08[工学] 09[农学] 0815[工学-水利工程] 090501[农学-动物遗传育种与繁殖] 

基　　金：This work was supported by grants from the National Natural Science Foundation of China (No.30700884 30873145) and the Key Science and Technology Project of Shandong Province (No.2010GGC10294 BS2009SW015 2012GSF12122).Acknowledgments: We would like to thank Professor Yan Lee of University of Pennsylvania for her work in revising our paper 

主　　题：ouabain proliferating cell nuclear antigen hypertension brain Na-K ATPase 

摘      要：Background Ouabain is a mammalian adrenocortical hormone that is involved in the pathogenesis of hypertension by inhibiting Na-K ATPase *** also participates in a variety of kinase-mediated signaling pathways associated with Na-K *** studies have shown that ouabain can cause cardiac remodeling independent of elevated blood pressure and that proliferating cell nuclear antigen （PCNA） plays a coordinating role for numerous proteins involved in multiple processes associated with DNA ***,we hypothesized that ouabain might play a role in the cerebral cortex through signaling pathways independent of *** PCNA might be involved in this *** Male Sprague-Dawley rats were treated with ouabain or with 0.9% nitric sodium as the control *** blood pressure was recorded *** four weeks of treatment,morphological changes in the cerebral cortex were analyzed using light and transmission electron *** expression of PCNA in the cerebral cortex was evaluated by immunohistochemistry,real time quantitative PCR,and Western *** After 4-week treatment,there was no significant difference in systolic blood pressure compared with the control group,but both structural deterioration and up-regulated expression of PCNA in the brain was induced by ouabain *** These results suggest that ouabain induces alterations in the brain structure,and this effect is independent of blood *** might be involved in the repair process of ouabain-induced brain damage.