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Mass Spectrometry-Based targeted metabolomics Revealed the Regulatory Roles of Magnesium on Biofilm Formation in Escherichia coli by Targeting Functional Metabolites

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Journal of Analysis and Testing 2022年第2期6卷 89-97页

作者： Tian-Yu Wang Rui Guo Long-Long Hu Jing-Jing Liu Hai-Tao Lu Key Laboratory of Systems Biomedicine(Ministry of Education) Shanghai Center for Systems BiomedicineShanghai Jiao Tong UniversityShanghai 200240China Laboratory for Functional metabolomics Science Shanghai Jiao Tong UniversityShanghai 200240China

Biofi lms often impose harmful infl uences in many niches involving food contamination,antibiotics resistance,and environmental ***,eradicating biofi lms remains diffi cultly because the formation mechanism of biofi lms is still incompletely clarifi ***,we attempted to explore the regulatory role of magnesium(Mg^(2+))on biofi lm formation in Escherichia coli(***)using phenotype visualization with targeted metabolomics *** found that Mg^(2+)could exert signifi cant infl uence on biofi lm formation with a concentration dependency by regulating phenotypic morphology and triggering metabolic modifi cations of biofi *** imaging revealed that increasing concentration of Mg^(2+)gradually inhibited biofi lm formation,Mg^(2+)was observed to restore the microstructure of *** strain in biofi lms to that in the relevant planktonic *** addition,our metabolomics analysis characterized 20 diff erential metabolites and associated two metabolic pathways including nucleotide metabolism and amino acid metabolism that were notably modifi ed during biofi lm formation under the treatments of varied Mg^(2+).Altogether,our work provides a novel insight into the infl uence of Mg^(2+)on biofi lm formation at a metabolic level,which is implicated in the novel solution to disturb biofi lm formation through the regulation of Mg^(2+)and functional metabolite interaction.

关键词： Biofilms Magnesium precision-targeted metabolomics Metabolic modifications Escherichia coli

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targeted metabolomics Revealed the Regulatory Role of Manganese on Small-Molecule Metabolism of Biofilm Formation in Escherichia coli

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Journal of Analysis and Testing 2020年第3期4卷 226-237页

作者： Rui Guo Haitao Lu Key Laboratory of Systems Biomedicine(Ministry of Education) Shanghai Center for Systems BiomedicineShanghai Jiao Tong UniversityShanghai 200240China Laboratory for Functional metabolomics Science Shanghai Jiao Tong UniversityShanghai 200240China

Biofilms are special microbial communities produced by many microorganisms,such as bacteria,viruses,and *** enable the microorganisms to possess the capacity against a diversity of stressful ***,biofilm formation often causes tough challenges in clinical infections,food quality,and environmental issues,however,the formation mechanism of biofilms are still incompletely understood which seriously impedes the development of new strategies to eradicate biofilms in different *** this study,we sought to explore the regulatory role of manganese(Mn^(2+))on small-molecule metabo-lism of biofilm formation in Escherichia coli(***).Using structural imaging assay combined with precision-targeted metabolomics method,to investigate how biofilm formation responded to various concentrations of Mn^(2+),we found that Mn^(2+)could inhibit biofilm formation through the regulation of phenotypic morphology and metabolic ***,our work discovered 16 differential functional metabolites and associated three metabolic pathways involving glycolysis,TCA cycle,and tryptophan metabolism that were changed mostly by Mn^(2+)during biofilm formation,which can differentiate biofilms from the relevant planktonic ***,this study demonstrated that Mn^(2+)can inhibit biofilm formation to regulate metabolic reprogramming and micro-structure,such effort provides novel insight into the regulation of metabolic features of biofilm formation,which enables the development of new strategies to eradicate biofilm formation for addressing the challenging problems in different areas by targeting the regulation of Mn^(2+)to the biosynthesis and expres-sions of functional metabolites produced by different microorganisms.

关键词： Biofilms Manganese precision-targeted metabolomics Imaging assay Small-molecule metabolism Escherichia coli

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