Mass Spectrometry-Based Targeted Metabolomics Revealed the Regulatory Roles of Magnesium on Biofilm Formation in Escherichia coli by Targeting Functional Metabolites

作     者：Tian-Yu Wang Rui Guo Long-Long Hu Jing-Jing Liu Hai-Tao Lu 

作者机构：Key Laboratory of Systems Biomedicine(Ministry of Education)Shanghai Center for Systems BiomedicineShanghai Jiao Tong UniversityShanghai 200240China Laboratory for Functional Metabolomics ScienceShanghai Jiao Tong UniversityShanghai 200240China 

出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)

年 卷 期：2022年第6卷第2期

页      面：89-97页

核心收录：

学科分类：0710[理学-生物学] 071011[理学-生物物理学] 1003[医学-口腔医学] 07[理学] 0806[工学-冶金工程] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：supported by the National Key R&D Program of China(2017YFC1308600 and 2017YFC1308605) the National Natural Science Foundation of China Grant(31670031) Natural Science Foundation of Shanghai(21ZR1431600) 

主　　题：Biofilms Magnesium Precision-targeted metabolomics Metabolic modifications Escherichia coli 

摘      要：Biofi lms often impose harmful infl uences in many niches involving food contamination,antibiotics resistance,and environmental ***,eradicating biofi lms remains diffi cultly because the formation mechanism of biofi lms is still incompletely clarifi ***,we attempted to explore the regulatory role of magnesium(Mg^(2+))on biofi lm formation in Escherichia coli(***)using phenotype visualization with targeted metabolomics *** found that Mg^(2+)could exert signifi cant infl uence on biofi lm formation with a concentration dependency by regulating phenotypic morphology and triggering metabolic modifi cations of biofi *** imaging revealed that increasing concentration of Mg^(2+)gradually inhibited biofi lm formation,Mg^(2+)was observed to restore the microstructure of *** strain in biofi lms to that in the relevant planktonic *** addition,our metabolomics analysis characterized 20 diff erential metabolites and associated two metabolic pathways including nucleotide metabolism and amino acid metabolism that were notably modifi ed during biofi lm formation under the treatments of varied Mg^(2+).Altogether,our work provides a novel insight into the infl uence of Mg^(2+)on biofi lm formation at a metabolic level,which is implicated in the novel solution to disturb biofi lm formation through the regulation of Mg^(2+)and functional metabolite interaction.