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HvMPK4 phosphorylates HvWRKY1 to enhance its suppression of barley immunity to powdery mildew fungus

Journal of Genetics and Genomics 2024年第3期51卷 313-325页

作者： Pengya Xue Ling Zhang Renchun Fan Yanan Li Xinyun Han Ting Qi Lifang Zhao Deshui Yu Qian-Hua Shen State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Biotic Interactions College of Advanced Agricultural SciencesUniversity of Chinese Academy of SciencesBeijing 100049China Department of Nutritional Sciences&Toxicology University of CaliforniaBerkeleyCA 94720USA Lushan Botanical Garden Chinese Academy of ScienceJiujiangJiangxi 332900China

Mitogen-activated protein kinase(MAPK)cascades play important roles in disease resistance in model plant species.However,the functions of MAPK signaling pathways in crop disease resistance are largely unknown.Here we report the function of HvMKK1-HvMPK4-HvWRKY1 module in barley immune system.HvMPK4 is identified to play a negative role in barley immune response against Bgh,as virus-induced gene silencing of HvMPK4 results in enhanced disease resistance whilst stably overexpressing HvMPK4 leads to super-susceptibility to Bgh infection.Furthermore,the barley MAPK kinase HvMKK1 is found to specifically interact with HvMPK4,and the activated HvMKK1^(DD) variant specifically phosphorylates HvMPK4 in vitro.Moreover,the transcription factor HvWRKY1 is identified to be a downstream target of HvMPK4 and phosphorylated by HvMPK4 in vitro in the presence of HvMKK1^(DD).Phosphorylation assay coupled with mutagenesis analyses identifies S122,T284,and S347 in HvWRKY1 as the major residues phosphorylated by HvMPK4.HvWRKY1 is phosphorylated in barley at the early stages of Bgh infection,which enhances its suppression on barley immunity likely due to enhanced DNA-binding and transcriptional repression activity.Our data suggest that the HvMKK1-HvMPK4 kinase pair acts upstream of HvWRKY1 to negatively regulate barley immunity against powdery mildew.

关键词： Barley MPK4 WRKY1 Phosphorylation Disease resistance powdery mildew

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Development and identification of two novel wheat-rye 6R derivative lines with adult-plant resistance to powdery mildew and high-yielding potential

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The Crop Journal 2024年第1期12卷 308-313页

作者： Guohao Han Jing Wang Hanwen Yan Tiantian Gu Lijun Cao Shiyu Liu Xiuquan Li Yilin Zhou Jieru Fan Zhipeng Shi Hong Liu Lihui Li Diaoguo An Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050021HebeiChina The National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China The State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China

powdery mildew,caused by Blumeria graminis f.***.tritici(Bgt),is a devastating disease that seriously threatens wheat yield and quality.To control this disease,host resistance is the most effective measure.Compared with the resistance genes from common wheat,alien resistance genes can better withstand infection of this highly variable pathogen.Development of elite alien germplasm resources with powdery mildew resistance and other key breeding traits is an attractive strategy in wheat breeding.In this study,three wheat-rye germplasm lines YT4-1,YT4-2,and YT4-3 were developed through hybridization between octoploid triticale and common wheat,out of which the lines YT4-1 and YT4-2 conferred adult-plant resistance(APR)to powdery mildew while the line YT4-3 was susceptible to powdery mildew during all of its growth stages.Using genomic in situ hybridization,multi-color fluorescence in situ hybridization,multi-color GISH,and molecular marker analysis,YT4-1,YT4-2,and YT4-3 were shown to be cytogenetically stable wheat-rye 6R addition and T1RS.1BL translocation line,6RL ditelosomic addition and T1RS.1BL translocation line,and T1RS.1BL translocation line,respectively.Compared with previously reported wheat-rye derivative lines carrying chromosome 6R,YT4-1 and YT4-2 showed stable APR without undesirable pleiotropic effects on agronomic traits.Therefore,these novel wheat-rye 6R derivative lines are expected to be promising bridge resources in wheat disease breeding.

关键词： powdery mildew Secale cereale Triticum aestivum Wheat-rye 6R derivatives Agronomic performance

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Effect of powdery mildew on interleaf microbial communities and leaf antioxidant enzyme systems

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林业研究:英文版 2023年第5期34卷 1535-1547页

作者： Xinbo Ma Zhanbin Wang Run Liu Yibing Jiang Northeast Forestry University Harbin 150040People’s Republic of China

Chinese peony(Paeonia lactiflora Pall.)is both medicinally and aesthetically beneficial.powdery mildew is a common fungal disease that seriously jeopardizes the value of numerous species,including peonies as a crop.In order to provide a basis for the prevention and treatment of peony powdery mildew,we examined the microbial diversity,the malondialdehyde(MDA)concentrations and antioxidant enzyme activities of peony leaves infected with three levels of powdery mildew to determine any modifications to the leaf's antioxidant enzyme systems and microbial community structure following the onset of disease.The results show that the MDA content rose as the degree of infection became worse.Antioxidant enzyme activity rose and then declined.Following the initiation of powdery mildew,fungal community diversity decreased,whereas there was not any appreciable change in bacterial communities according to microbial diversity sequencing.The relative abundance of more than half of fungal species decreased,with the bacterial genera displaying both abundant and diminished communities with less pronounced alterations in their community structure after the disease spread.Significant different taxa that were critical to the organization of each microbiome were found.Correlation analysis showed that the relative abundance of powdery mildew pathogenic fungal genus Erysiphe was correlated with those of 11 fungal genera and one bacterial genus.Among them,Aureobasidium,Neosetophoma and Sclerostagonospora showed significant positive correlations with Erysiphe and MDA.

关键词： powdery mildew Disease level Interleaf microbial diversity Antioxidant enzyme

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Fine mapping of an adult-plant resistance gene to powdery mildew in soybean cultivar Zhonghuang 24

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The Crop Journal 2022年第4期10卷 1103-1110页

作者： Qian Zhou Bingzhi Jiang Yanbo Cheng Qibin Ma Qiuju Xia Ze Jiang Zhandong Cai Hai Nian The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural UniversityGuangzhou 510642GuangdongChina The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology South China Agricultural UniversityGuangzhou 510642GuangdongChina The Key Laboratory of Plant Molecular Breeding of Guangdong Province College of AgricultureSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong Provincial Key Laboratory of Crops Genetics and Improvement Crops Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510642GuangdongChina Beijing Genomics Institute(BGI)Education Center University of Chinese Academy of SciencesShenzhen 518083GuangdongChina

powdery mildew(PM),caused by the fungus Microsphaera diffusa,causes severe yield losses in soybean[Glycine max(L.)Merr.]under suitable environmental conditions.Identifying resistance genes and developing resistant cultivars may prevent soybean PM damage.In this study,analysis of F_(1),F_(2),and F8:11 recombinant inbred line(RIL)populations derived from the cross between Zhonghuang 24(ZH24)and Huaxia 3(HX3)indicated that adult-plant resistance(APR)to powdery mildew in the soybean cultivar(cv.)ZH24 was controlled by a single dominant locus.A high-density genetic linkage map of the RIL population was used for fine mapping.The APR locus in ZH24 was mapped to a 281-kb genomic region on chromosome 16.Using 283 susceptible plants of another F2 population,the candidate region was finemapped to a 32.8-kb genomic interval flanked by the markers InDel14 and Gm16_428.The interval harbored five genes,including four disease resistance(R)-like genes,according to the Williams 82.a2.v1 reference genome.Quantitative real-time PCR assays of candidate genes revealed that the expression levels of Glyma.16g214300 and Glyma.16g214500 were changed by M.diffusa infection and might be involved in disease defense.Rmd_B13 showed all-stage resistance(ASR)to PM in soybean cv.B13.An allelism test in the F2 segregating population from the cross of ZH24 × B13 suggested that the APR locus Rmd_ZH24 and the ASR locus Rmd_B13 may be allelic or tightly linked.These results provide a reference marker-assisted selection in breeding programs.

关键词： Glycine max powdery mildew Adult-plant resistance Gene Fine mapping

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Breeding melon(Cucumis melo)with resistance to powdery mildew and downy mildew

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Horticultural Plant Journal 2022年第5期8卷 545-561页

作者： Lei Cui Lampros Siskos Chen Wang Henk J.Schouten Richard G.FVisser Yuling Bai College of Agriculture Shanxi Agricultural UniversityTaiyuan030031China Plant Breeding Wageningen University&ResearchP.O.Box 3866700 AJWageningenthe Netherlands

Melon(Cucumis melo L.)production is often restricted by a plethora of pests and diseases,including powdery mildew and downy mildew caused respectively by the fungal species Podosphaera xanthii/Golovinomyces orontii and oomycete species Pseudoperonospora cubensis.Many efforts have been directed on identification of resistant sources by screening(wild)melon germplasm.In the current review,we summarized such efforts from various publications of the last 50 plus years.Resistance to powdery mildew has been identified in 239 melon accessions and downy mildew resistance in 452 accessions of both C.melo and the wild relative species C.figarei.Among the resistance sources,C.melo var.cantalupensis accessions PMR 45,PMR 5,PMR 6,and WMR 29 as well as C.melo var.momordica accessions PI 124111,PI 124112,and PI 414723 have been considered as the most valuable germplasm because multiple resistance genes have been identified from these accessions and are widely used in melon resistance breeding.Further genetic mapping in a number of resistant sources has enabled identification of 25 dominant genes,two recessive genes and seven QTLs conferring powdery mildew resistance,as well as eight dominant genes and 11 QTLs for downy mildew resistances.Based on the reported sequences of associated markers,we anchored physically(many of)these genes and QTLs to chromosomes of the melon cv.DHL92 genome.In addition to presenting a comprehensive overview on powdery mildew and downy mildew resistance in(wild)melon germplasm,we suggest strategies aiming at breeding melon with durable and broad-spectrum resistance to pathogens and pests.

关键词： Cucumis melo Melon powdery mildew Downy mildew Resistance Breeding

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Effects of nutrient solution irrigation quantity and powdery mildew infection on the growth and physiological parameters of greenhouse cucumbers

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International Journal of Agricultural and Biological Engineering 2022年第2期15卷 68-74页

作者： Hanping Mao Yafei Wang Ning Yang Yong Liu Xiaodong Zhang School of Agricultural Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina Key Laboratory of Modern Agricultural Equipment and Technology Ministry of EducationJiangsu UniversityZhenjiang 212013JiangsuChina School of Electrical and Information Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina

For important economic crops such as cucumbers,abiotic and biotic stress conditions have had an adverse effect.This study aimed to observe the effects of water stress and powdery mildew stress on cucumber plants.Biotic stress at two levels,B0(no powdery mildew stress)and B1(powdery mildew stress)with three fertigation requirement levels(T1:Full fertigation,T2:Moderate nutrient solution deficit,T3:Severe nutrient solution deficit).Therefore,set up six treatments of B0T1,B1T2,B0T3,B1T1,B1T2,and B1T3.Leaf gas-exchange parameter first decreases and then increases under B1T1,B1T2,and B1T2.The leaf gas exchange parameter was significantly decreased under B0T2 and B0T3.Compared to the control experiment(CK,B0T1:no disease infestation and full irrigation),the plant height of cucumber decreased by 7.55%,10.62%,15.28%,23.27%,and 35.16%,respectively,under B0T2,B0T3,B1T1,B1T2 and B1T3;the stem diameter of cucumber decreased by 9.46%,15.74%,5.47%,13.45%,and 23.72%,respectively,under B0T2,B0T3,B1T1,B1T2,and B1T3;the leaf area of cucumber decreased by 13.76%,29.96%,13.43%,38.21%,and 66.83%,respectively,under B0T2,B0T3,B1T1,B1T2,and B1T3.The root-shoot ratio of cucumber was 3.92%,3.13%,3.63%,3.84%,4.41%,and 3.82%,respectively,under the B0T1,B0T2,B0T3,B1T1,B1T2,and B1T3.Therefore,this study can provide a basis for the research on greenhouse environmental control and cucumber cultivation management.

关键词： greenhouse cucumber powdery mildew nutrient solution drought stress growth traits physiological traits

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Identification and fine mapping of PmNJ3946 for powdery mildew resistance in einkorn wheat

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The Crop Journal 2023年第6期11卷 1846-1851页

作者： Peisi Wang Jun Huang Na Li Jie Zhang Caimei Gu Yang Yuan Ziruo Wen Haiyan Jia Zhongxin Kong Zhengqiang Ma Crop Genomics and Bioinformatics Center and National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095JiangsuChina Zhongshan Biological Breeding Laboratory Nanjing 210014JiangsuChina

powdery mildew caused by Blumeria graminis f.***.tritici(Bgt)is a destructive wheat disease.Although it can be easily overcome by deployment of resistance genes,the resistance is often quickly compromised by pathogen virulence.Thus,exploration and characterization of new resistance genes is always ongoing.Line NJ3946 derived from a cross of einkorn wheat accessions TA2032 and M389 showed resistance to powdery mildew.Inheritance analysis of an F2 population derived from a cross of NJ3946 and M389 suggested that the resistance was conferred by a dominant allele.With polymorphic markers identified through bulked segregant analysis(BSA),this gene was mapped to a novel locus on chromosome 3A,and was designated as PmNJ3946.Bulked segregant RNA-seq analysis(BSR-seq)was conducted to obtain more closely linked markers,which allowed delimitation of the PMNJ3946 locus to a 0.9 cM interval covering a physical distance of less than 1 Mb.PMNJ3946 was flanked by Xwgrc5153 and SNP-derived marker CHS21_3A008915069,and co-segregated with SNP-derived markers CHS21_3A008939814 and CHS21_3A008943175.The PmNJ3946 discovery expands the diversity of powdery mildew resistance genes and is useful for wheat breeding.

关键词： powdery mildew Disease resistance Genetic mapping Triticum monococcum PmNJ3946

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Ectopic Overexpression of EuCHIT30.7 Improves Nicotiana tabacum Resistance to powdery mildew

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Phyton-International Journal of Experimental Botany 2023年第11期92卷 3043-3061页

作者： Lanjun Li Degang Zhao Key Laboratory of Mountain Plant Resources Protection and Germplasm Innovation of the Ministry of Education College of Life Sciences/Institute of Agricultural BioengineeringGuizhou UniversityGuiyang550025China Plant Conservation Technology Center Guizhou Key Laboratory of Agricultural BiotechnologyBiotechnology Institute of GuizhouGuizhou Academy of Agricultural SciencesGuiyang550006China

Various strains of powdery mildew(PM),a notorious plant fungal disease,are prevalent and pose a significant threat to plant health.To control PM,transgenic technology can be used to cultivate more resistant plant varieties.In the present study,we utilized the rapid amplification of cDNA ends(RACE)technique to clone the full-length cDNA sequence of the EuCHIT30.7 gene to explore plant genes with disease resistance functions.Bioinformatics analysis revealed that this gene belongs to the GH18 family and is classified as a class III chitinase.The EuCHIT30.7 gene is expressed throughout the Eucommia ulmoides plant,with the most abundant expression in male flowers.Subcellular localization analysis indicated that the protein encoded by this gene was detected within both the cell membrane and cytoplasm.Upon PM inoculation,overexpression of EuCHIT30.7 in tobacco plants led to a significantly reduced relative lesion area and a decreased spore count compared to both wild-type and empty vector control plants.Activities of the protective enzymes,namely,peroxidase(POD),superoxide dismutase(SOD),catalase(CAT),and phenylalaninammo-nialyase(PAL),in tobacco plants overexpressing EuCHIT30.7 were significantly greater than those in wild-type and empty vector tobacco plants.Furthermore,the rate of increase in malondialdehyde(MDA)content was significantly lower in tobacco plants expressing EuCHIT30.7 compared to control tobacco plants.In EuCHIT30.7 transgenic tobacco,the expression of pathogen-related protein genes,namely,PR2,PR5,PR1a,PDF1.2,and MLP423,along with the tobacco PM negative regulatory gene,MLO2,were significantly higher compared to control tobacco plants.These findings suggested that EuCHIT30.7 significantly enhances the resistance of tobacco to PM.

关键词： Eucommia ulmoides Oliv. EuCHIT30.7 powdery mildew chitinase tobacco

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Suppression of edr2-mediated powdery mildew resistance, cell death and ethylene-induced senescence by mutations in ALD1 in Arabidopsis

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Journal of Genetics and Genomics 2011年第4期38卷 137-148页

作者： Haozhen Nie[a][b] Yingying Wu[a][b] Chunpeng Yao[a][b] Dingzhong Tang[a] [a] State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China [b] Graduate University of Chinese Academy of Sciences Beijing 100049 China

EDR2 is a negative regulator of the defense response and cell death in Arabidopsis.Loss-of-function of EDR2 leads to enhanced resistance to powdery mildew.To identify new components in the EDR2 signal transduction pathway,mutations that suppress edr2 resistant phenotypes were screened.Three mutants,edts5-1,edts5-2 and edts5-3（edr two suppressor 5）,were identified.The EDTS5 gene was identified by map-based cloning and previously was shown to encode an aminotransferase（ALD1）.Therefore we renamed these three alleles ald1-10,ald1-11 and ald1-12,respectively.Mutations in ALD1 suppressed all edr2-mediated phenotypes,including powdery mildew resistance,programmed cell death and ethylene-induced senescence.Accumulation of hydrogen peroxide in edr2 was also suppressed by ald1 mutation.The expression of defense-related genes was up-regulated in the edr2 mutant,and the up-regulation of those genes in edr2 was suppressed in the edr2/ald1 double mutant.The ald1 single mutant displayed delayed ethylene-induced senescence.In addition,ald1 mutation suppressed edr1-mediated powdery mildew resistance,but could not suppress the edr11edr2 double-mutant phenotype.These data demonstrate that ALD1 plays important roles in edr2-mediated defense responses and senescence,and revealed a crosstalk between ethylene and salicylic acid signaling mediated by ALD1 and EDR2.

关键词： ALD1 Ambidopsis Defense signaling EDR2 powdery mildew Salicylic acid

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Two major er1 alleles confer powdery mildew resistance in three pea cultivars bred in Yunnan Province, China

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The Crop Journal 2016年第5期4卷 353-359页

作者： Suli Sun Yuhua He Cheng Dai Canxing Duan Zhendong Zhu National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of Agricultural Sciences Yunnan Academy of Agricultural Sciences

powdery mildew, caused by Erysiphe pisi D.C., is an important disease of pea(Pisum sativum L.).The use of cultivars carrying powdery mildew resistance alleles at the er1 locus is the most effective and economical means of controlling this disease. The objectives of this study were to screen Chinese elite pea cultivars for resistance to E. pisi and to identify the responsible gene at the er1 locus. Among the 37 pea cultivars tested, three(Yunwan 8, Yunwan 21, and Yunwan 23) were immune to E. pisi infection in phenotypic evaluations. The full-length cD NA sequences of the er1 candidate gene, PsM LO1, from the three resistant cultivars and control plants were analyzed. Comparison of the cD NA sequences of 10 clones revealed differences among the powdery mildew-resistant cultivars, susceptible controls, and wild-type cultivar Sprinter. The observed resistance in Yunwan 8 plants resulted from a point mutation(C → G) at position 680 of PsM LO1 that introduced a stop codon, leading to premature termination of protein synthesis. The responsible resistance allele was identified as er1–1. powdery mildew resistance in Yunwan 21 and Yunwan 23 plants was caused by identical insertions or deletions in PsM LO1. Three distinct PsM LO1 transcripts were observed in Yunwan 21 and Yunwan 23 plants. These transcripts were characterized by a129-bp deletion and 155- and 220-bp insertions, respectively. The responsible resistance allele was identified as er1–2. We have characterized two important er1 alleles in three E. pisi-resistant pea cultivars bred in Yunnan Province, China. These cultivars represent important genetic resources for the breeding of powdery mildew-resistant pea cultivars.

关键词： Erysiphe pisi er1 Pea powdery mildew Yunnan Province

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