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3D printed pore morphology mediates bone marrow stem cell behaviors via RhoA/ROCK2 signaling pathway for accelerating bone regeneration

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Bioactive Materials 2023年第8期26卷 413-424页

作者： Qiji Lu Jingjing Diao Yingqu Wang Jianlang Feng Fansen Zeng Yan Yang Yudi Kuang Naru Zhao Yingjun Wang School of Materials Science and Engineering South China University of TechnologyGuangzhou510641PR China National Engineering Research Center for Tissue Restoration and Reconstruction South China University of TechnologyGuangzhou510006PR China NMPA Key Laboratory for Research and Evaluation of Innovative Biomaterials for Medical Devices.Guangzhou 510006PR China Medical Devices Research&Testing Center of SCUT Guangzhou510006PR China School of Biomedical Sciences and Engineering South China University of TechnologyGuangzhou International CampusGuangzhou511442PR China Guangdong Institute of Advanced Biomaterials and Medical Devices Guangzhou510535PR China

Bone bionics and structural engineering have sparked a broad interest in optimizing artificial scaffolds for better bone ***,the mechanism behind scaffold pore morphology-regulated bone regeneration remains unclear,making the structure design of scaffolds for bone repair *** address this issue,we have carefully assessed diverse cell behaviors of bone mesenchymal stem cells(BMSCs)on theβ-tricalcium phosphate(β-TCP)scaffolds with three representative pore morphologies(i.e.,cross column,diamond,and gyroid pore unit,respectively).Among the scaffolds,BMSCs on theβ-TCP scaffold with diamond pore unit(designated as D-scaffold)demonstrated enhanced cytoskeletal forces,elongated nucleus,faster cell mobility,and better osteogenic differentiation potential(for example,the alkaline phosphatase expression level in D-scaffold were 1.5-2 times higher than other groups).RNA-sequencing analysis and signaling pathway intervention revealed that Ras homolog gene family A(RhoA)/Rho-associated kinase-2(ROCK2)has in-depth participated in the pore morphology-mediated BMSCs behaviors,indicating an important role of mechanical signaling transduction in scaffold-cell ***,femoral condyle defect repair results showed that D-scaffold could effectively promote endogenous bone regeneration,of which the osteogenesis rate was 1.2-1.8 times higher than the other ***,this work provides insights into pore morphology-mediated bone regeneration mechanisms for developing novel bioadaptive scaffold designs.

关键词： Bone mesenchymal stem cells 3D-printed scaffold pore morphology Bone regeneration Structure-osteogenesis relationship

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Effects of coal molecular structure and pore morphology on methane adsorption and accumulation mechanism

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Frontiers of Earth Science 2023年第1期17卷 262-272页

作者： Jingshuo ZHANG Xiaoming NI Ying HAN Junfeng LIN School of Energy Science and Engineering Henan Polytechnic UniversityJiaozuo 454000China

The adsorption,diffusion,and aggregation of methane from coal are often studied based on slit or carbon nanotube models and isothermal adsorption and thermodynamics ***,the pore morphology of the slit model involves a single slit,and the carbon nanotube model does not consider the molecular structure of *** difference of the adsorption capacity of coal to methane was determined without considering the external environmental conditions by the molecular structure and pore morphology of *** study of methane adsorption by coal under single condition cannot reveal its *** view of this,elemental analysis,FTIR spectrum,XPS electron energy spectrum,13C NMR,and isothermal adsorption tests were conducted on the semi-anthracite of Changping mine and the anthracite of Sihe Mine in Shanxi Province,*** grand canonical Monte Carlo(GCMC)and molecular dynamics simulation method was used to establish the coal molecular structure *** comparing the results with the experimental test results,the accuracy and practicability of the molecular structure model are *** on the adsorption potential energy theory and aggregation model,the adsorption force of methane on aromatic ring structure,pyrrole nitrogen structure,aliphatic structure,and oxygen-containing functional group was *** relationship between pore morphology,methane aggregation morphology,and coal molecular structure was *** results show that the adsorption force of coal molecular structure on methane is as follows:aromatic ring structure(1.96 kcal/mol)>pyridine nitrogen(1.41 kcal/mol)>pyrrorole nitrogen(1.05 kcal/mol)>aliphatic structure(0.29 kcal/mol)>oxygen-containing functional group(0.20 kcal/mol).In the long and narrow regular pores of semi-anthracite and anthracite,methane aggregates in clusters at turns and aperture changes,and the adsorption and aggregation positions are mainly determined by the aromatic ring structure,the positions of pyrrole nitrog

关键词： Grand Canonical Monte Carlo pore morphology methane adsorption molecular structure of coal

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Effect of pore morphology and Regional Distribution of Liquid Diffusion Directionality in Nonwoven Fabrics

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Journal of Donghua University(English Edition) 2023年第3期40卷 267-276页

作者：殷炯周倩倩纪峰张瑞云王妮王永生 Key Laboratory of Textile Science&Technology Ministry of EducationDonghua UniversityShanghai 201620China China Chemical Fibers Association Beijing 100020China

The effect of pore morphology and regional distribution on liquid diffusion directionality in nonwoven fabrics was investigated in this *** orientation angle(POA) and pore aspect ratio(PAR) were proposed to characterize the pore morphology,and α-region,β-region,and αβ-region were used to describe the characteristics of the pore regional *** directional characteristics of macroscopic diffusion of liquid in nonwoven fabrics were characterized by the indicator of primary diffusion orientation angle(PDOA).Ten kinds of spunlaced nonwoven fabrics were ***,the data of pore characteristic indices of each sample were obtained through scanning electron microscope(SEM) and the image processing technology as well,and the pore regional distribution map of each sample was further ***,the PDOA of each sample was obtained through the droplet method and image processing *** on the data and statistical analysis,it was found that the PDOA of a certain volume of liquid in the nonwoven fabrics presented a significant linear relationship with the average POA of the nonwoven *** the characteristics of pore distribution affected the directionality of liquid diffusion in the nonwoven *** samples with a large proportion of α-region and good distribution had prominent liquid diffusion along the direction of laying-up,and the difference in liquid diffusion of the samples was more obvious between the directions of laying-up and vertical laying-up.

关键词： liquid diffusion anisotropy pore morphology regional distribution primary diffusion orientation angle(PDOA)

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Nanoscale pore morphology and distribution of lacustrine shale reservoirs:Examples from the Upper Triassic Yanchang Formation,Ordos Basin

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Journal of Energy Chemistry 2015年第4期24卷 512-519页

作者： Yang Wang Yanming Zhu Hongyan Wang Guangjun Feng Key Laboratory of Coalbed Methane Resources and Reservoir Formation Process Ministry of Education School of Resources and Earth Science China University of Mining and Technology Langfang Branch Petro China Exploration and Development Research Institute

pore structure plays an important role in the gas storage and flow capacity of shale gas reservoirs. Fieldemission environmental scanning electron microscopy（FE-SEM） in combination with low-pressure carbon dioxide gas adsorption（CO2GA）,nitrogen gas adsorption（N2GA）,and high-pressure mercury intrusion（HPMI） were used to study the nanostructure pore morphology and pore-size distributions（PSDs） of lacustrine shale from the Upper Triassic Yanchang Formation,Ordos Basin. Results show that the pores in the shale reservoirs are generally nanoscale and can be classified into four types： organic,interparticle,intraparticle,and microfracture. The interparticle pores between clay particles and organic-matter pores develop most often,l with pore sizes that vary from several to more than 100 nm. Mercury porosimetry analysis shows total porosities ranging between 1.93 and 7.68%,with a mean value of 5.27%. The BET surface areas as determined by N2 adsorption in the nine samples range from 10 to 20 m2/g and the CO2 equivalent surface areas（2 nm）vary from 18 to 71 m2/g. Together,the HPMI,N2 GA,and CO2 GA curves indicate that the pore volumes are mainly due to pores 100 nm in size. In contrast,however,most of the specific surface areas are provided by the micropores. The total organic carbon（TOC） and clay minerals are the primary controls of the structures of nanoscale pores（especially micropores and mesopores）. Micropores are predominantly determined by the content of the TOC,and mesopores are possibly related to the content of clay minerals,particularly the illite-montmorillonite mixed-layer content.

关键词： pore morphology pore-size distributions Lacustrine shale Yanchang Formation Ordos Basin

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Experimental on the pore structure characteristics of Longmaxi Formation shale in southern Sichuan Basin,China

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Petroleum 2021年第2期7卷 135-141页

作者： Linlin Huang Xiangjun Liu Jian Xiong Lixi Liang State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengduChina

In this paper,the pore structure characteristics of shale samples from the Lower Silurian Longmaxi Formation in South of Sichuan Basin of China were investigated by total organic carbon(TOC)content determination,X-ray diffraction(XRD),scanning electron microscope(SEM),low pressure nitrogen adsorption(LPNA)and high pressure mercury injection(HPMI).The fractal dimension of shale samples was calculated based on Frenkel-Halsey-Hill(FHH)model and thermodynamic relation *** results showed that the major mineral compositions of shales were quartz and clay *** pores,intergranular pores,intragranular pores,microfractures were widely developed in the shale samples,of which organic pores were the most *** pore morphology was mainly ink bottle-shaped pores and slit-shaped pores;the pore size distribution of shale samples was complex with multiple distribution peaks,the pore size between 3 and 40 nm occupied the most of storage *** fractal dimension Dn1 of pores between 2 nm and 10 nm was 2.7177e2.7933,while fractal dimension Dn2 of pores between 10 nm and 50 nm was *** fractal dimension Dr of macropores calculated by the thermodynamic model was 2.6401e2.7025.

关键词： The Longmaxi Formation Shale pore morphology pore size distribution Fractal dimension

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Preparation and characterization of porous titanium using space-holder technique

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Rare Metals 2009年第4期28卷 338-342页

作者： NIU Wenjuan BAI Chenguang QIU Guibao WANG Qiang WEN Liangying CHEN Dengfu DONG Lingyan College of Materials Science and Engineering Chongqing University Chongqing 400044 China

Titanium-based porous materials can be used in structural applications and medical implants because of their excellent mechanical properties at elevated temperatures, good corrosion resistance and wonderful biocompatibility. However, most of the methods used to produce the porous metal can only give limited porosity and uncontrollable pore morphologies. In the present study, a newly developed method of powder metallurgy using the space-holder technique was used to fabricate porous titanium with controllable porosity. The morphological features and mechanical properties of the products were fully investigated. The results show that the porosity is in the range of 55%-75%, and the mean pore size, with an average sphericity of -0.72, is 600 μm. The plateau stresses vary between 10 MPa and 35 MPa. As predicted by the Gibson-Ashby model, the plateau stress decreases with increasing porosity.

关键词： powder metallurgy porous titanium space-holder pore morphology mechanical properties

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Effect of Coated PHB on Properties of Abradable Seal Coating

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Journal of Wuhan University of Technology(Materials Science) 2014年第3期29卷 417-421页

作者：程旭东 XIANG Hongyu YE Weiping MENG Xiaoming MIN Jie LIU Minzhi ZHANG Pu LU Wei State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology College of Materials Science and Engineering Wuhan University of Technology

As pore-forming materials, the coated polyop-hydroxybenzoate（short for PHB） and h-BN can be applied in the preparation of abradable seal coatings at high temperature. The characteristics of coating such as morphology, thermal stability and composition were studied by SEM, EDS and FTIR. The results show that the modified PHB will change the remained carbon amount, porosity and pore morphology of the coating, which can affect the properties of coatings. If the pore is small enough in uniform distribution, the coating with 5 MPa bond strength, 30-55 HR45Y superficial hardness and certain of carbon can be suitable to well abradability.

关键词： coated-PHB pore morphology seal coating

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Using PEG as Progen to Preparate Chitosan Scaffold for Tissue Engineering

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Chinese Journal of Biomedical Engineering(English Edition) 2006年第3期15卷 134-138页

作者： Li Qin-hua Chen Jian-su Institute of ltiomedical Engineering Jinan University Guangzhou510632 China Medical College Jinan University Guangzhou510632 China

Tissue engineering basically made up growing the relevant cell in vitro and extracellular matrix. A major goal of tissue engineering is to preparate porous three dimension scaffold for cell proliferate, migrate, differention and to form the structure of desirable tissue and organ. In this study, the effects of various content and macromolecular weight of PEG to chitosan were investigated and evaluated. The pore morphology of chitosan was controlled by changing the concentration and macromolecular weight of PEG. Chitosan porous scaffold has interconecting porosity. The pore morphology can be controlled with varying PEG concentration and macromolecular weight. The pore size is between 10～50 urn, the degree of swelling in water is 85.70 % .

关键词： Chitosan scaffolds Porogen pore morphology

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