检索结果-南通市图书馆

Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress

Journal of Animal Science and Biotechnology 2022年第5期13卷 1326-1339页

作者： Meijie Song Yu Li Yihang Zhou Jiner Yan Xiaohua Zhou Qian Gao Yilong Miao Bo Xiong College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China College of Veterinary Medicine Nanjing Agricultural UniversityNanjing 210095China

Background:Elevated ambient temperature-caused heat stress is a major concern for livestock production due to its negative impact on animal feed intake,growth,reproduction,and ***,the germ cells are extremely sensitive to the heat ***,the effective approach and strategy regarding how to protect mammalian oocytes from heat stress-induced defects have not been ***:Germinal vesicle(GV)porcine oocytes were cultured at 41.5℃ for 24 h to induce heat stress,and then cultured at 38.5℃ to the specific developmental stage for subsequent *** mononucleotide(NMN)was dissolved in water to 1 mol/L for a stock solution and further diluted with the maturation medium to the final concentrations of 10μmol/L,20μmol/L,50μmol/L or 100μmol/L,respectively,during heat *** and fluorescence intensity quantification were applied to assess the effects of heat stress and NMN supplementation on the key processes during the oocyte meiotic ***:Here,we report that NMN supplementation improves the quality of porcine oocytes under heat ***,we found that heat stress resulted in oocyte maturation failure by disturbing the dynamics of meiotic organelles,including the cytoskeleton assembly,cortical granule distribution and mitochondrial *** addition,heat stress induced the production of excessive reactive oxygen species(ROS)and DNA damage,leading to the occurrence of apoptosis in oocytes and subsequent embryonic development *** importantly,we validated that supplementation of NMN during heat stress restored the meiotic defects during porcine oocyte ***:Taken together,our study documents that NMN supplementation is an effective approach to improve the quality of oocytes under heat stress by promoting both nuclear and cytoplasmic maturation.

关键词： Heat stress Meiotic defects Nicotinamide mononucleotide Oocyte quality porcine oocytes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Casein kinase 2 modulates the spindle assembly checkpoint to orchestrate porcine oocyte meiotic progression

引用

Journal of Animal Science and Biotechnology 2020年第3期11卷 715-723页

作者： Xiayan ShiYang Yilong Miao Zhaokang Cui Yajuan Lu Changyin Zhou Yu Zhang Bo Xiong College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China

Background:CK2(casein kinase 2)is a serine/threonine-selective protein kinase that has been involved in a variety of cellular processes such as DNA repair,cell cycle control and circadian rhythm ***,its functional roles in oocyte meiosis have not been fully ***:We report that CK2 is essential for porcine oocyte meiotic maturation by regulating spindle assembly checkpoint(SAC).Immunostaining and immunoblotting analysis showed that CK2 was constantly expressed and located on the chromosomes during the entire oocyte meiotic *** of CK2 activity by its selective inhibitor CX-4945 impaired the first polar body extrusion and arrested oocytes at M I stage,accompanied by the presence of BubR1 at kinetochores,indicative of activated *** addition,we found that spindle/chromosome structure was disrupted in CK2-inhibited oocytes due to the weakened microtubule stability,which is a major cause resulting in the activation of ***,we found that the level DNA damage as assessed byγH2A.X staining was considerably elevated when CK2 was inhibited,suggesting that DNA damage might be another critical factor leading to the SAC activation and meiotic failure of ***:Our findings demonstrate that CK2 promotes the porcine oocyte maturation by ensuring normal spindle assembly and DNA damage repair.

关键词： CK2 CX-4945 DNA damage Meiotic maturation porcine oocytes Spindle assembly

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Screening high-quality fetal bovine serum for porcine oocyte maturation in vitro

引用

Animal Models and Experimental Medicine 2019年第4期2卷 334-339页

作者： Xueqing Liu Qiaoli Lang Meng Wu Xiaoyan You Qiling He Ling Luo Zijia Liu Puying Xiao Nan Huang Xi Yang Liangpeng Ge Chongqing Academy of Animal Sciences ChongqingChina Key Laboratory of Pig Industry Sciences Ministry of AgricultureChongqingChina Key Laboratory of Pig Industry Sciences ChongqingChina Technical Engineering Center for the Development and Utilization of Medical Animal Resources ChongqingChina

Fetal bovine serum(FBS) is widely used in cell cultures due to its high stability and easy access. It was also used as a substitute for porcine follicular fluid(PFF) in previous studies. However, FBS components are unclear, and the presence of FBS in culture media may introduce a variation from batch to batch. This study aimed to establish an effective method to screen FBS in place of PFF in the culture media for porcine oocytes in vitro. We screened FBS from different sources by using porcine fetal fibroblast cells. The effects of six FBS samples on porcine fetal fibroblast cell growth were tested via frozen cell survival assay, cell clone formation assay, cell growth curve, and cell passage activity assay. The best serum that we called GFBS(heat-inactivated FBS, cat. no. 10500-64;Gibco) showed a similar effect on the maturation and development of porcine oocytes to that of PFF and can be used as a good substitute for PFF. These results suggested that the porcine fetal fibroblast cell culture test can be used as a valuable method to screen FBS for porcine oocyte maturation and embryonic development in vitro.

关键词： fetal bovine serum maturation rate porcine oocytes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：