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platelet-rich plasma enhances adipose-derived stem cell-mediated angiogenesis in a mouse ischemic hindlimb model

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World Journal of Stem Cells 2018年第12期10卷 212-227页

作者： Chia-Fang Chen Han-Tsung Liao Department of Plastic and Reconstructive Surgery Chang Gung Memorial Hospital Craniofacial Research Center Chang Gung Memorial Hospital College of Medicine Chang Gung University

AIM To evaluate the angiogenic effect of platelet-rich plasma(PRP)-preconditioned adipose-derived stem cells(ADSCs) both in vitro and in a mouse ischemic hindlimb model.METHODS ADSCs were divided based on culture medium: 2.5% PRP, 5% PRP, 7.5% PRP, and 10% PRP. Cell proliferation rate was analyzed using the MTS assay. The gene expression of CD31, vascular endothelial growth factor, hypoxia-inducible factors, and endothelial cell nitric oxide synthase was analyzed using reverse transcription polymerase chain reaction. Cell markers and structural changes were assessed through immunofluorescence staining and the tube formation assay. Subsequently, we studied the in vivo angiogenic capabilities of ADSCs by a mouse ischemic hindlimb model.RESULTS The proliferation rate of ADSCs was higher in the 2.5%, 5%, and 7.5% PRP groups. The expression of hypoxia-inducible factor, CD31, vascular endothelial growth factor, and endothelial cell nitric oxide synthase in the 5% and 7.5% PRP groups increased. The 5%, 7.5%, and 10% PRP groups showed higher abilities to promote both CD31 and vascular endothelial growth factor production and tubular structure formation in ADSCs. According to laser Doppler perfusion scan, the perfusion ratios of ischemic limb to normal limb were significantly higher in 5% PRP, 7.5% PRP, and human umbilical vein endothelial cells groups compared with the negative control and fetal bovine serum(FBS) groups(0.88 ± 0.08, 0.85 ± 0.07 and 0.81 ± 0.06 for 5%, 7.5% PRP and human umbilical vein endothelial cells compared with 0.42 ± 0.17 and 0.54 ± 0.14 for the negative control and FBS, P < 0.01).CONCLUSION PRP-preconditioned ADSCs presented endothelial cell characteristics in vitro and significantly improved neovascularization in ischemic hindlimbs. The optimal angiogenic effect occurred in 5% PRP-and 7.5% PRPpreconditioned ADSCs.

关键词： platelet-rich plasma Adipose-derived stem cells Mesenchymal stem cell Angiogenesis Endothelial differentiation Mouse ischemic hindlimb model

Single-and double-dose of platelet-rich plasma versus hyaluronic acid for treatment of knee osteoarthritis: A randomized controlled trial

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World Journal of Orthopedics 2019年第9期10卷 310-326页

作者： Mehdi Tavassoli Nasser Janmohammadi Akram Hosseini Soraya Khafri Seyed Mokhtar Esmaeilnejad-Ganji Student Research Committee School of MedicineBabol University of Medical SciencesBabol 47176-47745Iran Department of Orthopedics Babol University of Medical SciencesBabol 47176-47745Iran Department of Pathology Faculty of MedicineBabol University of Medical SciencesBabol 47176-47745Iran Biostatistics and Epidemiology Department Medicine FacultyBabol University of Medical SciencesBabol 47176-47745Iran Clinical Research Development Center Shahid Beheshti HospitalBabol University of Medical SciencesBabol 47176-47745Iran

BACKGROUND platelet-rich plasma(PRP)and hyaluronic acid have been shown to be useful in the treatment of knee osteoarthritis.However,investigations comparing the efficacy of these two drugs together are insufficient.AIM To compare the outcomes of PRP vs hyaluronic acid injections in three groups of patients with bilateral knee osteoarthritis.METHODS This randomized controlled trial study involved 95 patients.Thirty-one subjects received a single injection of PRP(group PRP-1),33 subjects received two injections of PRP at an interval of 3 wk(group PRP-2)and 31 subjects received three injections of hyaluronic acid at 1-wk intervals(group hyaluronic acid).The patients were investigated prospectively at the enrollment and at 4-,8-and 12-wk follow-up with the Western Ontario and McMaster Universities Arthritis Index(WOMAC)and Visual Analogue Scale questionnaires.RESULTS Percentages of patients experiencing at least a 30%decrease in the total score for the WOMAC pain subscale from baseline to wk 12 of the intervention were 86%,100%and 0%in the groups PRP-1,PRP-2 and hyaluronic acid,respectively(P<0.001).The mean total WOMAC scores for groups PRP-1,PRP-2 and hyaluronic acid at baseline were 63.71,61.57 and 63.11,respectively.The WOMAC scores were significantly improved at final follow-up to 42.5,35.32 and 57.26,respectively.The highest efficacy of PRP was observed in both groups at wk 4 with about 50%decrease in the symptoms compared with about 25%decrease for hyaluronic acid.Group PRP-2 had higher efficacy than group PRP-1.No major adverse effects were found during the study.CONCLUSION PRP is a safe and efficient therapeutic option for treatment of knee osteoarthritis.It was demonstrated to be significantly better than hyaluronic acid.We also found that the efficacy of PRP increases after multiple injections.

关键词： platelet-rich plasma Hyaluronic acid Osteoarthritis Knee Pain

Submucosal injection of platelet-rich plasma in endoscopic resection of large sessile lesions

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World Journal of Gastrointestinal Endoscopy 2018年第11期10卷 348-353页

作者： Vicente Lorenzo-Zuniga Vicente Moreno de Vega Ramón Bartolí Ingrid Marín NoemíCaballero Ignacio Bon Jaume Boix Department of Gastrointestinal Endoscopy University Hospital Germans Trias/CIBERehdBarcelona 08916Spain

AIM To prospectively evaluate the efficacy of submucosal injection of platelet-rich plasma(PRP) on endoscopic resection of large sessile lesions.METHODS Eleven patients were submitted to endoscopic mucosal resection(EMR) with prior injection of PRP, obtained at the time of endoscopy. Patients were followed during 1 mo. The incidence of adverse events(delayed bleeding or perforation) and the percentage of mucosal healing(MHR) after 4 wk were registered. RESULTS EMR was performed in 11 lesions(46.4 mm ± 4 mm, range 40-70 mm). Delayed bleeding or perforation was not observed in any patient. Mean ulcerated area atbaseline was 22.7 cm^2 ± 11.7 cm^2 whereas at week 4 were 2.9 cm^2 ± 1.5 cm^2. Patients treated with PRP showed a very high MHR after 4 wk(87.5%). CONCLUSION PRP is an easy-to-obtain solution with proven and favourable biological activities that could be used in advanced endoscopic resection.

关键词： platelet-rich plasma endoscopic mucosal resection submucosal injection large lesions

Establishing proof of concept:platelet-rich plasma and bone marrow aspirate concentrate may improve cartilage repair following surgical treatment for osteochondral lesions of the talus

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World Journal of Orthopedics 2012年第7期3卷 101-108页

作者： Niall A Smyth Christopher D Murawski Amgad M Haleem Charles P Hannon Ian Savage-Elliott John G Kennedy Department of Orthopaedic Surgery Hospital for Special SurgeryNew YorkNY 10021United States

Osteochondral lesions of the talus are common injuries in the athletic patient. They present a challenging clinical problem as cartilage has a poor potential for healing. Current surgical treatments consist of reparative(microfracture) or replacement(autologous osteochondral graft) strategies and demonstrate good clinical outcomes at the short and medium term follow-up. Radiological findings and second-look arthroscopy however, indicate possible poor cartilage repair with evidence of fibrous infill and fissuring of the regenerative tissue following microfracture. Longer-term follow-up echoes these findings as it demonstrates a decline in clinical outcome. The nature of the cartilage repair that occurs for an osteochondral graft to become integrated with the native surround tissue is also of concern. Studies have shown evidence of poor cartilage integration,with chondrocyte death at the periphery of the graft, possibly causing cyst formation due to synovial fluid ingress. Biological adjuncts, in the form of platelet-rich plasma(PRP) and bone marrow aspirate concentrate(BMAC), have been investigated with regard to their potential in improving cartilage repair in both in vitro and in vitro settings. The in vitro literature indicates that these biological adjuncts may increase chondrocyte proliferation as well as synthetic capability, while limiting the catabolic effects of an inflammatory joint environment. These findings have been extrapolated to in vitro animal models, with results showing that both PRP and BMAC improve cartilage repair. The basic science literature therefore establishes the proof of concept that biological adjuncts may improve cartilage repair when used in conjunction with reparative and replacement treatment strategies for osteochondral lesions of the talus.

关键词： Osteochondral lesion Cartilage repair platelet-rich plasma Bone marrow aspirate concentrate

The Security of platelet-rich Plasma in Sports-relatedInjuries

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Journal of Health Science 2014年第8期2卷 363-369页

作者： Leticia Rivero Gonzalez Gabriel Lupon Escobar LluisTil Perez Sara Estevez Sarmiento Fuerteventura Hospital Clinica Roca Sports Medical Unit Gran Canaria Hospital Universitario Insular de Gran Canaria LasPalmas de Gran Canaria Las Palmas 35016 Spain Sports Medicine Consorci Sanitari de Terrassa (CST) in Olympic Training Center (CAR) Sant Cugat del Vall~s 08173 Spain Sports Medicine FC Barcelona Barcelona 08028 Spain Hospital Universitario Insular de Gran Canaria Las Palmas de Gran Canaria Las Palmas 35016 Spain

platelet-rich plasma （PRP） therapy in orthopedics and sports medicine has seen an expansion in use since 2003. Theobjective of this study is to not only review evidence of clinical effectiveness but also to evaluate its security and detect any disparitybetween its use and its popularity. A prospective, descriptive study, which included 104 athletes were performed. Minor adverse effectswere recorded and these were self-limited. Although there is a lack of more detailed studies which compare other factors, PRP therapyhas been shown to have positive results in relation to certain lesions.

关键词： Sports medicine, growth factors, muscles, tendons, ligaments, platelet-rich plasma.

Effect of Autologus platelet-rich Plasma on IL-6, MMP-3 and MCP-1 Expression in Synoviocytes from Osteoarthritic Patients Knees

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Open Journal of Regenerative Medicine 2014年第3期3卷 64-72页

作者： Chang Ich Hur Cheol Park Hanlou Li Jong Keun Seon Hyun Keun Kim Taek Rim Yoon Eun-Kyoo Song Center for Joint Disease Chonnam National University Hwasun Hospital Jeonnam South Korea Fraunhofer Institute for Cell Therapy and Immunology Leipzig Germany Cardiovascular Convergence Research Center of Chonnam National University Gwangju South Korea

Nowadays, some studies reported promising results of platelet-rich plasma (PRP) for the treatment of osteoarthritis (OA). However, the effects of PRP on prevention of osteoarthritis in knee joints have been debated. The present study investigated the effects of PRP on osteoarthritis related inflammatory cytokines expressed in fibroblast-like synoviocytes (FLS) from osteoarthritic knees. The synovial tissues were harvested from eight osteoarthritic patients who had undertaken total knee arthroplasties (TKAs) and cultured in DMEM containing 10% FBS. platelet-rich plasma releasate (PRPr) was made by clotting or activation of PRP by citrate. The levels of PDGF-AA and VEGF in PRPr and whole blood were measured with ELISA method. The FLS were isolated and cultured from osteoarthritic knees. The IL-1β stimulated FLS were cultivated with three different conditions (none, 1% and 10% of PRP). To determine the expression of IL-6, MMP-3, and MCP-1, we used reverse transcriptase polymerase chain reaction (RT-PCR). The concentrations of platelet count in PRP were about 7 to 9 times higher than those of whole blood. The levels of PDGF-AA in PRPr were approximately 3 to 4 times higher than those of whole blood. The levels of VEGF in PRPr were also significantly 7 to 18 times higher than those of whole blood. Without induction of the FLS with IL-1β, 1% or 10% PRPr did not reduce expressions of inflammatory proteins (MMP-3, MCP-1), except for IL-6. However, with induction of the FLS with IL-1β, both concentrations (1% and 10%) of PRPr reduced significantly all inflammatory protein expressions (IL-6, MMP-3, MCP-1). PRPr diminished inflammatory IL-1β-mediated effects on human osteoarthritic fibroblast-like synoviocytes. These results suggest that platelet-rich plasma can be a good therapeutic option for the treatment of osteoarthritis.

关键词： platelet-rich Plasma Inflammatory Cytokines Synoviocyte Osteoarthritis

Subtenon Injection of Autologous platelet-rich Plasma in Retinitis Pigmentosa: Is It a New Therapeutic Option?

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Open Journal of Ophthalmology 2020年第1期10卷 77-88页

作者： Neslihan Sinim Kahraman Ayse Oner Ophthalmology Department of Ophthalmology Ac&#305badem Kayseri Hospital Kayseri Turkey

Purpose: Growth factors (GFs) and neurotrophins can decelerate retinal degeneration. This study aimed to investigate the safety, efficacy and durability of subtenon injections of autologous platelet-rich plasma (aPRP) which is a rich source of GFs in retinitis pigmentosa (RP) patients. Methods: 154 eyes of 77 RP patients with various degrees of narrowed visual field were investigated in this study. Each patient received three injections with 4-week intervals and followed for at least 12 months. The examinations and the tests were obtained before the injection, 1 month after the third injection and every 3 months during the study. The primary aim was to evaluate the effects of aPRP on visual acuity (VA) and visual functions, the second aim was to estimate the duration of the therapy effect and the need for additional aPRP injection. Results: Median age of the 77 RP patients was 35.2 ± 13.9 years. All of the eyes received 3 monthly bilateral subtenon aPRP injections. Of these patients 26 received an additional one, 12 received additional two and 1 patient received additional 3 injections with 3-month interval. There were no significant ocular or systemic side effects. The mean baseline VA was 0.22 ± 0.18 Snellen lines. It improved to 0.31 ± 0.19 following three aPRP injections, which was statistically significant. At the end of the study period, the mean VA was 0.27 ± 0.22 Snellen lines. Conclusion: The subtenon injection of aPRP might be an effective therapy and might have a positive influence in the preservation of visual functions and visual acuity of RP patients.

关键词： Retinitis Pigmentosa Growth Factors platelet-rich Plasma Visual Functions

Vacuum Assisted Closure (VAC) and platelet-rich Plasma (PRP): A Successful Combination in a Challenging Case of Gastroschisis

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Open Journal of Pediatrics 2015年第3期5卷 274-278页

作者： Vincenzo Domenichelli Simona Straziuso Maria Domenica Sabatino Silvana Federici Pediatric Surgical Unit “Infermi” Hospital AUSL Romagna Rimini Italy

Giant gastroschisis could be a surgical challenge concerning the abdominal wall reconstruction. Many techniques have been described for both primary or staged closure but sometimes neither of them is succesful in all patients. We are presenting the combined use of Vacuum Assisted Closure (VAC) and platelet-rich Plasma (PRP) to improve the result in this difficult case. The use of VAC device is a well known procedure in the treatment of adult difficult wounds closure. It consists of a sponge applied directly on the abdominal wall defect, covered with a transparent dressing and connected to a controlled continuous negative pressure system [1]. platelet-rich plasma (PRP) is an autologous concentration of human platelets in a small volume of plasma. Due to this combination it provides multiple growth and healing factors actively secreted by platelets which have been shown to begin and accelerate wound healing [2] [3]. The association between VAC and PRP was effective in the shrinkage and reduction of the abdominal defect. Fifteen months after the removal of the VAC device the fascia appears competent with a cutaneus scar that will need a plastic correction in the future. The VAC should be considered as a helpful and effective device in case of complicated giant gastroschisis or omphalocele when traditional treatment is not sufficient.

关键词： Vacuum Assisted Closure Gastroschisis platelet-rich Plasma Abdominal Wall Defect

Effects of kidney-tonifying and blood circulation-promoting recipes and platelet-rich plasma on the activity and expression of type I collagen

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Journal of Hainan Medical University 2019年第20期25卷 1-5页

作者： Ling-Hui Li Kai-Ming Li Shao-Feng Yang Li-Guo Zhu Shang-Quan Wang Jia-Wen Zhan Ming Chen Qing Zhang Department of Orthopaedics Wangjing HospitalChina Academy of Chinese Medical SciencesBeijing100102 Beijing key laboratory of orthopedics of traditional Chinese Medicine Beijing100102 The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine ChangshaHunan410007 Department of Spinal Surgery Wangjing HospitalChina Academy of Chinese Medical SciencesBeijing100102

Objective:To observe the effects of kidney-tonifying and blood circulation-promoting recipes and platelet-rich plasma(PRP)on the activity and expression of type I collagen in co-culture system.Methods:The subcutaneous adipose tissue and degenerative nucleus pulposus tissue of patients with nucleus pulposus removal under lumbar intervertebral disc degeneration were isolated and cultured by multiple enzyme digestion methods to establish a co-culture system of ADSCs/NPCs.Patients with lumbar intervertebral disc degeneration were enrolled for 2 weeks.The venous blood was taken 50mL before the first dose and one hour after the last dose.platelet-rich plasma was prepared by secondary centrifugation and cultured with different plasma.The experiment was divided into five groups:platelet-rich plasma group(PRP),platelet-poor plasma group(PPP),drug-rich platelet-rich plasma group(M-PRP)and drug-poor platelet plasma group(M-PPP),fetal bovine serum group(FBS).The morphological changes of the cells in each group were observed under an inverted microscope.The changes of type I collagen activity were detected by immunofluorescence assay.The expression of CCOL1A1 gene was detected by RT-PCR two weeks after the intervention.Results:In this study,the results of kidney-tonifying and blood circulation-promoting recipes and PRP group intervention were shown.The results of the group intervention showed that the M-PRP group and the PRP group had clearer cell contours and better refractive index than the other groups,and there was no significant difference between the two groups.The results of immunofluorescence staining showed that the number of cells in PRP group and M-PRP group was higher,but the staining intensity of single cells was significantly lower than that in FBS group.RT-PCR results showed that the expression of COL1A1 mRNA in PPP group,PRP group,M-PRP group and M-PPP group was significantly down-regulated(P<0.05),respectively:0.343±0.040,0.294±0.018,0.310±0.022,0.430±0.020.Conclusions:Kidney-tonifying and blood circulation-p

关键词： kidney-tonifying and blood circulation-promoting recipes platelet-rich plasma I collagen Activity expression