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New plant growth regulator Emistim C AS used on Watermelon in Field Evaluation

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Agricultural Science & Technology 2017年第2期18卷 247-249页

作者： Zhongliang LIU Juan DOU Jianli ZHENG Junjie GAO Taian Academy of Agricultural Sciences Taian 271000China Economic Crops Station Bureau of Agriculture in LanshanLinyi 27600China

Most plant growth regulators available are synthesized and tend to be dangerous if used inappropriately. Emistim CAS is a natural plant growth regulator, with less side-effect. The research performed an assessment on Emistim CAS application in watermelon cultivation in a greenhouse. The results showed that 0.11% Emistim CAS increased reducing sugar by 15.49%, soluble solid by 13.7%, Vc content by 3.72%, titratable acid by 18.1%, and yield per hectare by 18.01%, so that watermelon quality and yield were both improved. Emistim CAS can be widely promoted in watermelon cultivation in future.

关键词： plant growth regulator Emistim C AS Watermelon Quality Output

Effects of plant growth regulator on Absorption,Circulation and Contents of Potassium in Flue-cured Tobacco

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Agricultural Science & Technology 2011年第12期12卷 1877-1880,1982页

作者：沈方科韦忠王蕾尹永强潘炜玲顾明华广西大学农学院广西南宁530005 广西壮族自治区烟草公司河池市公司广西河池547000 贺州市农产品质量安全检测中心广西贺州542800

[Objective] This study was to investigate the effects of plant growth regulator on accumulation and circulation of potassium in flue-cured tobacco.[Methods] Hydroponics experiment was adopted to study the effects of plant growth regulator（NAA,GA3,BR） on accumulation and circulation of potassium in flue-cured tobacco.[Results] The BR treatment increased the absorption of potassium,calcium and magnesium in flue-cured tobacco,reduced the potassium emission from the root,enhanced the accumulation and contents and promoted the circulation of potassium in the tobacco *** was no statistical difference between the NAA and GA3 *** BR treatment was 0.52%,0.30%,0.28% higher than NAA treatment in enhancing potassium content in tobacco plants at 2,7,12 d after *** a word,the results showed that BR treatment was the most.[Conclusion] BR treatment could effectively enhance potassium content in tobacco plants after topping.

关键词： Flue-cured tobacco Absorption of potassium Potassium circulation plant growth regulator

Ethanol Metabolism in Calluses of Several Selected plant Species on Two Typical plant-growth-regulator Balanced Media

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Wuhan University Journal of Natural Sciences 2000年第4期5卷 500-504页

作者： Yang Yue-sheng, Zheng Ying-dong, Chen Yu-fen, Zheng Ling College of Biotechnology, South China Agricultural University, Guangzhou 510642, China College of Biotechnology South China Agricultural UniversityGuangzhou 510642China

For investigation on the characteristics of ethanol metabolism in tissues of different plant species, calluses from eight selected plant species were cultured on medium supplemented with ethanol in tightly sealed culture flasks. Changes of the ethanol level were detected by gas chromatography. During the culture period, the calluses of tobacco, potato and petunia were, able to catabolize exogenous ethanol, resulting in the prominent decline of the ethanol level in the medium. The calluses of melon and peanut were also able to catabolize thanol but with lower efficiency. The other three calluses of carrot, soybean and rice did not catabolize ethanol but instead produced small to large amount of ethanol, resulting in the increase of the ethanol level in the media. It was also found that changing the balance between auxin and cytokinin could influence only the ethanol metabolism efficiency but could not change the metabolism patterns on ethanol of the cultured calluses. It can be concluded that, ethanol metabolism pattern of calluses in cultures is an innate physiological characteristic of the respective plant species.

关键词： callus culture ethanol metabolism plant growth regulator plant physiology

New plant growth regulators for Fibre Flax

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Journal of Pharmacy and Pharmacology 2014年第3期2卷 194-200页

作者： Elena J. Bakhtenko Yury A. Suslov Tatyana V. Hurshkajnen Pavel B. Kurapov Department of botany Vologda State Pedagogical University Vologda 160600 Russia Department of Organic Synthesis and Chemistry of Natural Compounds Institute of Chemistry Komi Centre of Science UralBranch of the Russian Academy of Sciences Syktyvkar Komi 167982 Russia Department of Medical Nanobiotechnologies Pirogov Russian State Medical University Moscow 11799 7 Russia

Tests of new natural non-polluting regulators of growth Verva （vegetative terpenoids from Abies sibirica） and Larixin （vegetative flavonoids from Larix） on growth, development and productivity of fibre flax are conducted. The level of phytohormones in vegetating plants （IAA, ABA, zeatine and zeatinriboside） is studied. Preparations stimulate germination of seeds and growth of plants. Field experiment fixed yield increase of flax straw and the seeds of flax-fibre with the use of growth regulators. Industrial experiment represents VERVA product effectiveness on yields increase of flax straw, seeds and flax fiber output.

关键词： plant growth regulator terpenoids fibre flax phytohormones zeatine zeatinriboside abscisic acid 3-indolylacetic acid.

Influences of Carbon Sources and plant growth regulators on Anther Culture Efficiency of Pepper

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Agricultural Science & Technology 2010年第4期11卷 102-105页

作者：赵激邹学校张竹青杨博智周书栋湖南省农科院蔬菜研究所湖南长沙410125 中南大学研究生院隆平分院湖南长沙410125 湖南省农科院湖南长沙410125

[Objective]In order to increase anther culture efficiency of pepper.[Method]MS culture media and Bolajiaohong were used in this experiment to study the influences of carbon sources and concentrations on anther callus induction of *** was taken as a material to study influences of plant growth regulators and concentrations on anther callus induction of pepper according to L16（4^5） orthogonal design.[Result]The average callus and embryoid induction rates of maltose at all concentrations were higher than these of sucrose but the difference was not *** maltose or sucrose as a carbon source,3% to 6% concentration was good for increasing induction frequencies of calli and ***,If the concentration was over 6%,the induction rates were declined dramatically with the increase of sugar *** influences of growth regulators on induction rate of calli were listed as 2,4-D﹥ZT﹥NAA﹥KT﹥6-BA;the influences on induction rates of embryoids were listed as 2,4-D﹥NAA﹥ZT﹥KT﹥*** 2,4-D,ZT,NAA and KT had signficant or extremely significant influences on induction rates of calli and embryoids.2,4-D,ZT at 1.0 mg/L and NNA,KT at 0.5 mg/L had the best *** influences of ZT on calli and embryoids were better than those of KT and 6-BA.1.0 mg/L 2,4-D ＋1.0 mg/L ZT ＋0.5 mg/L KT ＋0.5 mg/L 6-BA was the best regulator combination for induction culture of Jiayu anther.[Conclusion]The experiment provided research basis for anther culture of pepper.

关键词： Pepper Anther culture Carbon source plant growth regulator Callus Embryoid

Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings

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Journal of Forestry Research 2022年第5期33卷 1573-1584页

作者： Leandro Silva de Oliveira Gilvano Ebling Brondani Letícia Vaz Molinari Rafaella Zanetti Dias Gustavo Leal Teixeira Antônio Natal Gonçalves Marcílio de Almeida Institute of Agricultural Science Federal University of Minas GeraisUniversity Campus1000Universitária Ave.Montes ClarosMG 39404-547Brazil Laboratory of In Vitro Culture of Forest Species Department of Forestry SciencesFederal University of LavrasUniversity CampusCentral AvenueLavrasMG 37200-900Brazil Department of Biological Sciences “Luiz de Queiroz”College of AgricultureUniversity of São Paulo(ESALQ/USP)University Campus11Pádua Dias AvenuePiracicabaSP 13418-900Brazil

Shoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana *** enables large-scale production of plants from transformed *** study evaluates the effect ofα-naphthaleneacetic acid(NAA),thidiazuron(TDZ)and benzylaminopurine(BAP)on the organogenesis of *** from hypocotyls and cotyledonary *** the induction stage,hypocotyls and cotyledonary leaves were established in a Murashige and Skoog(MS)culture medium supplemented with NAA or *** tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation *** buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89μM BAP and 0.05μM *** leaves exhibited the best in vitro *** induction of adventitious buds occurred only in calluses induced from *** the differentiation stage,4.4μM BAP treatment promoted an increase of adventitious bud ***-cuttings from regenerated shoots were acclimatized and rooted ex vitro in *** results confirm the establishment of an efficient protocol for the in vitro regeneration of *** by indirect organogenesis,providing new insights regarding cloning of this species.

关键词： Micropropagation Recalcitrant species plant growth regulator Explants source

Effects of Three plant growth regulators on Yield and Quality of Sunflower

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Agricultural Biotechnology 2016年第5期5卷 8-11页

作者： Hanhua LI Wei DUAN Anyang XU Hui WU Sumei WAN Tao YANG Xinjiang Kangdi Seed Industry Science and Technology Co. Ltd. College of plant Science Tarim University College of Forestry and Horticulture Xinjiang Agricultural University

Using Xinshikui No. 5 as the experimental material, the effects of different concentrations of mepiquat chloride （100, 200, 300,400, 500 mg/L）, chlormequat chloride （800, 1200,1 600, 2 000, 2 400 rag/L） and paclobutracol （50, 75, 100, 125, 150 mg/L） on yield and quality of sunflower were investigated. The results showed that plant growth regulators could improve the yield and quality of sunflower seeds to varying degrees. Specifically, 400 mg/L mepiquat chloride （ D4 ）, 800 mg/L chlormequat chloride （ Cl ） and 75 mg/L paelobutracol （ P2 ） significantly improved disk diameter, seed weight per disc, 100-sced weight, seed kernel rate and yield of sunflower; 400 mg/L mepiquat chloride （ D4 ）, 1 200 mg/L chlormequat chloride （ C2 ） and 75 mg/L paclobutracol （ P2 ） improved crude protein content, crude fat content, length and width of sunflower seeds. Based on comprehensive consideration, paclobutraeol is the most appropriate plant growth regulator for improving the yield and quality of sunflower in Xinjiang, and the optimal application concentration is 75 mg/L.

关键词： Sunflower plant growth regulator Yield Quality

Simultaneous Determination of plant growth regulators in Fruits Using a Modified Qu ECh ERS Procedure and UPLC–MS/MS

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Horticultural plant Journal 2016年第4期2卷 203-208页

作者： YAN Zhen NIE Jiyun XU Guofeng LI Haifei LI Jing LI Zhixia WU Yonglong KUANG Lixue Institute of Pomology Chinese Academy of Agricultural SciencesQuality Inspection and Test Center for Fruit and Nursery StocksLaboratory of Quality and Safety Risk Assessment for Fruit (Xingcheng)Ministry of Agriculture

Six plant growth regulators frequently used in fruit were detected using UPLC–MS/MS including chlormequat chloride, GA3, forchlorfenuron,paclobutrazol, thidiazuron and 2,4-D. A modified Qu ECh ERS method was used in pretreatment: each compound was extracted with acetonitrile containing 1% acetic acid and cleaned with a mixture of ostade-cylsilane(ODS) and Mg SO_4. The detection was performed using electrospray ionization(ESI) and multiple reaction monitoring mode(MRM) and quantified using the external standard method. Results indicated that the calibration curves of six plant growth regulators were linear within the range of 1–100 μg·kg^(-1) with correlation coefficients over 0.999. The recovery at four spiked concentrations, 1, 20, 50, and 100 μg·kg^(-1), ranged from 60.77% to 119.43% and relative standard deviation(RSD) were between 0.18% and 32.94%. The limits of detection(LOD) and limits of quantitation(LOQ) were 0.01–0.14 μg·kg^(-1) and 0.02–0.46 μg·kg^(-1), respectively, for apples, 0.01–0.18 μg·kg^(-1) and 0.03–0.59 μg·kg^(-1) for grapes, 0.01–0.08 μg·kg^(-1) and 0.04–0.28 μg·kg^(-1) for kiwis, 0.003–0.110 μg·kg^(-1) and 0.01–0.38 μg·kg^(-1) for peaches, and 0.01–0.08 μg·kg^(-1) and 0.03–0.25 μg·kg^(-1) for oranges.

关键词： fruit plant growth regulator Qu ECh ERS UPLC–MS/MS

Research Advances in Detection Methods of plant growth regulators for Agricultural Products

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Agricultural Biotechnology 2021年第3期10卷 71-75页

作者： Shuyuan XU Lei WANG Haitao ZHAO Huihui CAO Shuai WANG Sining TANG Jianhua WANG Tangshan Food and Drug Comprehensive Testing Center Tangshan 063000China Tangshan Institute of Industrial Technology for Functional Agricultural Products Tangshan 063000China Hebei Agricultural Products Quality and Safety Testing Innovation Center Tangshan 063000China Hebei Institute of Industrial Technology for Peanut Tangshan 063000China

In recent years,plant growth regulators are widely used in agricultural *** the toxicity of plant growth regulator residues has gained increasing concerns,trace analysis methods for plant growth regulators have been *** this paper,the major methods with advantages and disadvantages for the detection and pre-treatment of plant growth regulator residues in agricultural products were summarized,including gas chromatography(GC),high performance liquid chromatography(HPLC),chromatographic technique combined with mass spectrometry,enzyme-linked immunosorbent assay(ELISA),capillary electrophoresis(CE)and so ***,the development prospects were also discussed.

关键词： Agricultural products plant growth regulator Detection method