检索结果-南通市图书馆

A general method for assaying homo-and hetero-transglycanase activities that act on plant cell-wall polysaccharides

Journal of Integrative plant Biology 2015年第4期57卷 411-428页

作者： Lenka Franková Stephen C.Fry The Edinburgh cell wall Group Institute of Molecular Plant SciencesThe University of EdinburghDaniel Rutherford BuildingThe King's Buildings

Transglycanases（endotransglycosylases） cleave a polysaccharide（donor-substrate） in mid-chain, and then transfer a portion onto another poly-or oligosaccharide（acceptor-substrate）. Such enzymes contribute to plant cellwall assembly and/or re-structuring. We sought a general method for revealing novel homo- and hetero-transglycanases, applicable to diverse polysaccharides and oligosaccharides, separating transglycanase-generated3 Hpolysaccharides from unreacted3H-oligosaccharides—the former immobilized（on filter-paper, silica-gel or glassfiber）,the latter eluted. On filter-paper, certain polysaccharides [e.g.（1!3, 1!4）-b-D-glucans] remained satisfactorily adsorbed when water-washed; others（e.g. pectins） were partially lost. Many oligosaccharides（e.g. arabinan-, galactan-, xyloglucan-based） were successfully eluted in appropriate solvents, but others（e.g. [3H]xylohexaitol, [3H]mannohexaitol[3H]cellohexaitol） remained immobile. On silica-gel, all3 Holigosaccharides left an immobile ‘ghost’ spot（contaminating any3H-polysaccharides）, which was diminished but not prevented by additives e.g. sucrose or Triton X-100. The best stratum was glassfiber（GF）, onto which the reactionmixture was dried then washed in 75% ethanol. Washing led to minimal loss or lateral migration of3H-polysaccharides if conducted by slow percolation of acidified ethanol. The effectiveness of GF-blotting was well demonstrated for Chara vulgaris transb-mannanase. In conclusion, our novel GF-blotting technique ef ficiently frees transglycanase-generated3H-polysaccharides from unreacted3H-oligosaccharides,enabling high-throughput screening of multiple postulated transglycanase activities utilising chemically diverse donorand acceptor-substrates.

关键词： Glass-fiber blotting plant cell wall polysaccharides（donor） radiolabeled oligosaccharides （acceptor） transglycanase

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying plant cell wall Formation

引用

Molecular plant 2010年第5期3卷 818-833页

作者： Xiaohong Zhu Sivakumar Pattathil Koushik Mazumder Amanda Brehm Michael G. Hahn S.P. Dinesh-Kumar Chandrashekhar P. Joshi Biotechnology Research Center School of Forest Resources and Environmental Science Michigan Technological University Houghton MI 49931 USA BioEnergy Science Center Complex Carbohydrate Research Center University of Georgia 315 Riverbend Road Athens GA 30602 USA Department of plant Biology University of Georgia Athens GA 30602 USA Section of plant Biology and Genome Center University of California Davis CA 95616 USA Department of Bioenergy Science and Technology Chonnam National University Gwangju 500-757 Korea

Virus-induced gene silencing （VIGS） is a powerful genetic tool for rapid assessment of plant gene functions in the post-genomic era. Here, we successfully implemented a Tobacco Rattle Virus （TRV）-based VlGS system to study functions of genes involved in either primary or secondary cell wall formation in Nicotiana benthamiana plants. A 3-week post- VIGS time frame is sufficient to observe phenotypic alterations in the anatomical structure of stems and chemical composition of the primary and secondary cell walls. We used cell wall glycan-directed monoclonal antibodies to demonstrate that alteration of cell wall polymer synthesis during the secondary growth phase of VIGS plants has profound effects on the extractability of components from woody stem cell walls. Therefore, TRV-based VlGS together with cell wall component profiling methods provide a high-throughput gene discovery platform for studying plant cell wall formation from a bioenergy perspective.

关键词： plant cell wall VIGS cellulose xylan lignin Nicotiana.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

plant cell wall, a Challenge for Its Characterisation

引用

Advances in Biological Chemistry 2016年第3期6卷 70-105页

作者： Guy Costa Idelette Plazanet Laboratoire de Chimie des Substances Naturelles (EA 1069) Faculté des Sciences et Techniques Limoges Cedex France

The plant cell wall is a complex 3D network composed of polysaccharides, lignin and proteins. The knowledge of the structure and content of each cell wall polymer is a prerequisite to understand their functions during plant development and adaptation but also to optimise their industrial applications. The analysis of cell wall compounds is complicated by their multiple molecular interactions. In this review, we present numerous methods to purify, characterise and quantify proteins, polysaccharides and lignin from the wall. Two kinds of approaches are detailed: the first presents in vitro methods which involve the breakdown of the molecular linkages between polymers thanking to chemical, physical and/or enzymatic treatments. The second approach describes in situ methods that allow the cell wall polymer characterisation thanking to many analytical techniques coupled with microscopy. If microscopy is the common point of all of them, their development is associated with improvement of analytical techniques, increasing their power of resolution.

关键词： Polysaccharides Proteins Lignin Purification Spectroscopy Chromatography Immunology Microscopy plant cell wall

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dietary fiber in plant cell walls-the healthy carbohydrates

引用

Food Quality and Safety 2022年第2期6卷 125-141页

作者：安轶陆伟泰李文泽潘浪浪卢孟柱 Igor Cesarino 李争曾为 State Key Laboratory of Subtropical Silviculture College of Forestry and BiotechnologyZhejiang A&F UniversityHangzhouChina State Key Labor atory for Conservation and Utilization of Bio-Resources in Yunnan School of AgricultureYunnan UniversityKunmingChina Department of Botany Institute of BiosciencesUniversity of Sao PauloRua do MataoSao PauloBrazil

Dietary fiber(DF)is one of the major classes of nutrients for humans.It is widely distributed in the edible parts of natural plants,with the cell wall being the main DF-containing structure.DF content varies significantly in different plant species and organs,and the processing procedure can have a dramatic effect on the DF composition of plant-based foods.Given the considerable nutritional value of DF,a deeper understanding of DF in food plants,including its composition and biosynthesis,is fundamental to the establishment of a daily intake reference of DF and is also critical to molecular breeding programs for modifying DF content.In the past decades,plant cell wall biology has seen dramatic progress,and such knowledge is of great potential to be translated into DF-related food science research and may provide future research directions for improving the health benefits of food crops.In this review,to spark interdisciplinary discussions between food science researchers and plant cell wall biologists,we focus on a specific category of DF--cell wall carbohydrates.We first summarize the content and composition of carbohydrate DF in various plant-based foods,and then discuss the structure and biosynthesis mechanism of each carbohydrate DF category,in particular the respective biosynthetic enzymes.Health impacts of DF are highlighted,and finally,future directions of DF research are also briefly outlined.

关键词： Dietary fiber carbohydrate plant cell wall polysaccharide glycosyltransferases healthy food

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Systematic analysis of plant cell wall structures towards a fundamental model on biomass production and application in crops

Systematic analysis of plant cell wall structures towards a ...

引用

第十四届全国植物基因组学大会

作者： Liangcai Peng Guosheng Xie Tao Xia Fengcheng Li Yangting Wang National Key Laboratory of Crop Genetic Improvement Biomass and Bioenergy Research CentreCollege of Plant Sciences and TechnologyHuazhong Agricultural University

plant cell wall determines plant mechanic strength and other biological properties,and also represents an enormous biomass resource for biofuel and chemical products.In this study,we performed integrative and comparat... 详细信息

关键词： plant cell wall genomics and metabolism network biomass production and biofuels genetic engineering of energy crops

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Physiological Response of the Resurrection Fern Subjected to Environmental Stress as Shown by plant cell Membrane Integrity

引用

American Journal of plant Sciences 2022年第8期13卷 1081-1089页

作者： Carly Balsam Ed Bush St. Joseph’s Academy Baton Rouge USA LSU AgCenter Baton Rouge USA

Resurrection fern has a unique ability to maintain cell wall integrity when the plant cell is desiccated. It uses proteins such as late embryogenesis proteins and heat shock proteins to maintain their cellular functions. The purpose of this experiment is to determine the effects of environmental stressors on the physiological response of the resurrection fern (Pleopeltis polypodioides). The physiological response of resurrection fern plants was subjected to various temperatures (-50°C, 0°C, 25°C, and 50°C) for 7 days. Results indicated that there was a significant difference between hydrated and desiccated ferns based on the temperature. Additionally, electrolyte leakage measurements confirmed cell damage following exposure to temperature extremes of -50°C and 50°C.

关键词： Adaptive Mechanisms plant Stress plant cell wall cell Leakage

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A novel mitochondrial protein is required for cell wall integrity,auxin accumulation and root elongation in Arabidopsis under salt stress

引用

Stress Biology 2022年第1期2卷 530-544页

作者： Zheping Yu Yuying Ren Jianwei Liu Jian-Kang Zhu Chunzhao Zhao Institute of Horticulture Zhejiang Academy of Agricultural SciencesHangzhou 310021China Shanghai Center for plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China

Maintenance of root elongation is beneficial for the growth and survival of plants under salt stress,but currently the cellular components involved in the regulation of root growth under high salinity are not fully understood.In this study,we identified an Arabidopsis mutant,rres1,which exhibited reduced root elongation under treatment of a variety of salts,including NaCl,NaNO3,KCl,and KNO3.RRES1 encodes a novel mitochondrial protein and its molecular function is still unknown.Under salt stress,the root meristem length was shorter in the rres1 mutant compared to the wild type,which was correlated with a reduced auxin accumulation in the mutant.Reactive oxygen species(ROS),as important signals that regulate root elongation,were accumulated to higher levels in the rres1 mutant than the wild type after salt treatment.Measurement of monosaccharides in the cell wall showed that arabinose and xylose contents were decreased in the rres1 mutant under salt stress,and application of boric acid,which is required for the crosslinking of pectic polysaccharide rhamnogalacturonan-II(RG-II),largely rescued the root growth arrest of the rres1 mutant,suggesting that RRES1 participates in the maintenance of cell wall integrity under salt stress.GUS staining assay indicated that the RRES1 gene was expressed in leaves and weakly in root tip under normal conditions,but its expression was dramatically increased in leaves and roots after salt treatment.Together,our study reveals a novel mitochondrial protein that regulates root elongation under salt stress via the modulation of cell wall integrity,auxin accumulation,and ROS homeostasis.

关键词： Salt stress Auxin Reactive oxygen species plant cell wall Arabidopsis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Elucidating the role of SlXTH5 in tomato fruit softening

引用

Horticultural plant Journal 2023年第4期9卷 777-788页

作者： Duoduo Wang Qianhui Lu Xiaomin Wang Hui Ling Ning Huang College of Life Sciences Zhejiang Normal UniversityJinhuaZhejiang 321004China Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic plants Zhejiang Normal UniversityJinhuaZhejiang 321004China Department of plant Science School of BiosciencesUniversity of NottinghamLoughboroughLE125RDUK Research Centre for Germplasm Innovation and Application of Guangxi Agricultural and Forestry plant Resourse College of AgricultureYulin Normal UniversityYulinGuangxi 537000China

Fruit softening in tomato(Solanum lycopersicum)is closely associated with cell wall disassembly,which is brought about through the action of a range of cell wall structure-related enzymes and other proteins such as expansins.Xyloglucan endotransglucosylase/hydrolase(XTH)(EC 2.***.1.207 and/or EC 3.***.1.151)has been proposed to be key player involved in xyloglucan metabolism.SlXTH5 showed the highest expression level among all SlXTHs during tomato ripening.In this study,the role of SlXTH5 involved in tomato softening was investigated in CRISPR-based knockout mutants of SlXTH5.Loss-of-function of SlXTH5 in transgenic tomato lines resulted in slightly firmer fruit pericarp,but significantly decreased their color index compared with azygous wild type(WT)control fruits.Increased paste viscosity was detected in CRISPR mutants,indicating that the activity of SlXTH5 is responsible for maintaining cell wall structural integrity.Immunocytochemistry studies were performed using the monoclonal antibody probe LM25 to examine the localization and distribution of xyloglucan in the pericarp cells of the CRISPR mutant fruits.The data indicated more xyloglucan was retained in the pericarp of CRISPR mutant fruit than in WT control fruit.This study revealed the link between SlXTH5 and xyloglucan metabolism and indicated the potential of manipulating SlXTH5 to regulate fruit softening.

关键词： Tomato Fruit ripening SlXTH5 Immunocytochemistry Xyloglucan plant cell wall

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

cellulose synthesis in land plants

引用

Molecular plant 2023年第7期16卷 206-231页

作者： Gustav B.Pedersen Leonard Blaschek Kristian E.H.Frandsen Lise C.Noack Staffan Persson Copenhagen plant Science Center(CPSC) Department of Plant&Environmental SciencesUniversity of CopenhagenThorvaldsensvej 401871Frederiksberg CDenmark Joint International Research Laboratory of Metabolic&Developmental Sciences State Key Laboratory of Hybrid RiceSJTU-University of Adelaide Joint Centre for Agriculture and HealthSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina

All plant cells are surrounded by a cell wall that provides cohesion,protection,and a means of directional growth to plants.cellulose microfibrils contribute the main biomechanical scaffold for most of these walls.The biosynthesis of cellulose,which typically is the most prominent constituent of the cell wall and therefore Earth’s most abundant biopolymer,is finely attuned to developmental and environmental cues.Our understanding of the machinery that catalyzes and regulates cellulose biosynthesis has substantially improved due to recent technological advances in,for example,structural biology and microscopy.Here,we provide a comprehensive overview of the structure,function,and regulation of the cellulose synthesis machinery and its regulatory interactors.We aim to highlight important knowledge gaps in the field,and outline emerging approaches that promise a means to close those gaps.

关键词： plant cell wall cellulose microfibrils cellulose synthases membrane proteins cytoskeleton protein interaction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Targeting and Regulation of cell wall Synthesis During Tip Growth in plants

引用

Journal of Integrative plant Biology 2013年第9期55卷 835-846页

作者： Fangwei Gu Erik Nielsen Department of Molecular Cellular and Developmental Biology University of MichiganAnn Arbor Michigan 48109 USA

Root hairs and pollen tubes are formed through tip growth, a process requiring synthesis of new cell wall material and the precise targeting and integration of these components to a selected apical plasma membrane domain in the growing tips of these cells. Presence of a tip-focused calcium gradient, control of actin cytoskeleton dynamics, and formation and targeting of secretory vesicles are essential to tip growth. Similar to cells undergoing diffuse growth, cellulose, hemi-celluloses, and pectins are also deposited in the growing apices of tip-growing cells. However, differences in the manner in which these cell wall components are targeted and inserted in the expanding portion of tip-growing cells is reflected by the identification of elements of the plant cell wall synthesis machinery which have been shown to play unique roles in tip-growing cells. In this review, we summarize our current understanding of the tip growth process, with a particular focus on the subcellular targeting of newly synthesized cell wall components, and their roles in this form of plant cell expansion.

关键词： cellulose membrane trafficking plant cell wall pollen tube root hair tip growth.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：