Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying Plant Cell Wall Formation

作     者：Xiaohong Zhu Sivakumar Pattathil Koushik Mazumder Amanda Brehm Michael G. Hahn S.P. Dinesh-Kumar Chandrashekhar P. Joshi 

作者机构：Biotechnology Research Center School of Forest Resources and Environmental Science Michigan Technological University Houghton MI 49931 USA BioEnergy Science Center Complex Carbohydrate Research Center University of Georgia 315 Riverbend Road Athens GA 30602 USA Department of Plant Biology University of Georgia Athens GA 30602 USA Section of Plant Biology and Genome Center University of California Davis CA 95616 USA Department of Bioenergy Science and Technology Chonnam National University Gwangju 500-757 Korea 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2010年第3卷第5期

页      面：818-833页

核心收录：

学科分类：0710[理学-生物学] 090601[农学-基础兽医学] 07[理学] 08[工学] 09[农学] 0906[农学-兽医学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：NSF CAREER program USDA-NRI program CPBR funding World Class University project of the Ministry of Science and Technology of Korea NSF Plant Genome Program BioEnergy Science Center 

主　　题：Plant cell wall VIGS cellulose xylan lignin Nicotiana. 

摘      要：Virus-induced gene silencing （VIGS） is a powerful genetic tool for rapid assessment of plant gene functions in the post-genomic era. Here, we successfully implemented a Tobacco Rattle Virus （TRV）-based VlGS system to study functions of genes involved in either primary or secondary cell wall formation in Nicotiana benthamiana plants. A 3-week post- VIGS time frame is sufficient to observe phenotypic alterations in the anatomical structure of stems and chemical composition of the primary and secondary cell walls. We used cell wall glycan-directed monoclonal antibodies to demonstrate that alteration of cell wall polymer synthesis during the secondary growth phase of VIGS plants has profound effects on the extractability of components from woody stem cell walls. Therefore, TRV-based VlGS together with cell wall component profiling methods provide a high-throughput gene discovery platform for studying plant cell wall formation from a bioenergy perspective.