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Highly permeable reverse osmosis membranes incorporated with hydrophilic polymers of intrinsic microporosity via interfacial polymerization

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Chinese Journal of Chemical Engineering 2022年第5期35卷 194-202页

作者： Jing Dou Shuo Han Saisai Lin Zhikan Yao Lian Hou Lin Zhang Engineering Research Center of Membrane and Water Treatment of MOE College of Chemical&Biological EngineeringZhejiang UniversityHangzhou 310027China Research Institute of Ningbo Zhejiang UniversityNingbo 315100China Xi’an High-Tech Institute Xi’an 710025China

Enhancing the water permeation while maintaining high salt rejection of existing reverse osmosis(RO)membranes remains a considerable challenge.Herein,we proposed to introduce polymer of intrinsic microporosity,pim-1,into the selective layer of reverse osmosis membranes to break the trade-off effect between permeability and selectivity.A water-soluble a-Lpim-1 of low-molecular-weight and hydroxyl terminals was synthesized.These designed characteristics endowed it with high solubility and reactivity.Then it was mixed with m-phenylenediamine and together served as aqueous monomer to react with organic monomer of trimesoyl chloride via interfacial polymerization.The characterization results exhibited that more“nodule”rather than“leaf”structure formed on RO membrane surface,which indicated that the introduction of the high free-volume of a-Lpim-1 with three dimensional twisted and folded structure into the selective layer effectively caused the frustrated packing between polymer chains.In virtue of this effect,even with reduced surface roughness and unchanged layer thickness,the water permeability of prepared reverse osmosis membranes increased 2.1 times to 62.8 L·m^(-2)·h^(-1) with acceptable Na Cl rejection of 97.6%.This attempt developed a new strategy to break the trade-off effect faced by traditional polyamide reverse osmosis membranes.

关键词： pim-1 Intrinsic microporosity Reverse osmosis Interfacial polymerization Trade-off

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Expressions of Osteopontin (OPN),αVβ3 and pim-1 Associated with Poor Prognosis in Non-small Cell Lung Cancer (NSCLC)

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Chinese Journal of Cancer Research 2012年第2期24卷 103-108页

作者： Yi Jin Da-yue Tong Lu-ying Tang Jian-ning Chen Jing Zhou Zhi-ying Feng Chun-kui Shao Department of Pathology the Third Affiliated HospitalSun Yet-sen UniversityGuangzhou 510630China Department of Forensic medicine ZhongShan Medical School Sun Yet-sen UniversityGuangzhou 520080China

Objective： To examine the expressions ot osteopontin （OPN）, αvP3 and pim-1 in non-small cell lung cancer （NSCLC）, and investigate their potential pathogenic roles in the development of NSCLC. Methods： Immunohistochemistry was used to examine the expressions of OPN, αve3 and pim-1 in cohort （136 cases） of NSCLC samples and their adjacent normal lung tissue specimens. Statistical analysis was performed to evaluate the relationships among expressions of OPN, αve3 and pim-1 and their associations with patients clinico- pathological parameters. Results： The expressions of OPN and pim-1 were predominantly observed in cytoplasm. The expression of αve3 was mostly detected in cytoplasm and/or membrane. In NSCLC samples, the positive rates of OPN, αve3 and pim-1 expressions were 68.4% （93/136）, 77.2% （105/136） and 57.4% （78/136）, respectively. In normal lung tissues, in contrast, the positive rates of OPN, αve3 and pim-1 were 24.0% （12/50）, 26.0% （13/50） and 16.0% （8/50）, respectively. There were significant differences of the positive expression rates of OPN, αve3 and pim-1 between NSCLCs samples and normal lung tissues （P〈O.01）. In addition, the positive expression of OPN, αve3 and pim-1 in NSCLCs samples was significantly associated with increased pathological grade, lymph node metastasis and advanced clinical stage （P〈O.01）, and they were independent of other clinicopathological parameters （P〉0.05）. Furthermore, a significantly positive correlation between the expression of OPN and αve3 （r=0.38, P〈O.O1）, OPN and pim-1 （r=0.37, P〈O.01）, or av133 and pim-1 （r=0.20, P〈0.05） was evaluated in our NSCLC cohort. Conclusion： OPN, αve3 and pim-1 proteins are frequently overexpressed in NSCLC, and they may play important roles in the development and/or progression of NSCLC.

关键词： NSCLC OPN αvl33 pim-1 Immunohistochemistry

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pim-1 Kinase Regulating Dynamics Related Protein 1 Mediates Sevoflurane Postconditioning-induced Cardioprotection

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Chinese Medical Journal 2017年第3期130卷 309-317页

作者： Jin-Dong Liu Hui-Juan Chen Da-Liang Wang Hui Wang Qian Deng Department of Anesthesiology The Affiliated Hospital of Xuzhou Medical University Jiangsu Province Key Laboratory of Anesthesiology Xuzhou Medical University Xuzhou Jiangsu 221004 China Department of Anesthesiology Jiangsu Province Key Laboratory of Anesthesiology Xuzhou Medical University Xuzhou Jiangsu 221004 China

Background： It is well documented that sevoflurane postconditioning （SP） has a significant myocardial protection effect. However, the mechanisms underlying SP are still unclear. In the present study, we investigated the hypothesis that the pim-1 kinase played a key role in SP-induced cardioprotection by regulating dynamics-related protein 1 （Drpl）. Methods： A Langendorff model was used in this study. Seventy-two rats were randomly assigned into six groups as follows： CON group, ischemia reperfusion （I/R） group, SP group, SP＋proto-oncogene serine/threonine-protein kinase 1 （pim-1） inhibitor II group, SP＋dimethylsufoxide group, and pim- 1 inhibitor II group （n = 12, each）. Hemodynamic parameters and infarct size were measured to reflect the extent of myocardial 1/R injury. The expressions ofpim-1, B-cell leukemia/lymphoma 2 （Bcl-2） and cytochrome C （Cyt C） in cytoplasm and mitochondria, the Drp 1 in mitochondria, and the total Drp I and p-Drp I^sor637 were measured by Western blotting. In addition, transmission electron microscope was used to observe mitochondrial morphology. The experiment began in October 2014 and continued until July 2016. Results： SP improved myocardial I/R injury-induced hemodynamic parametric changes, cardiac function, and preserved mitochondrial phenotype and decreased myocardial infarct size （24.49 ± 1.72% in Sev group compared with 41.98 ± 4.37% in I/R group; P 〈 0.05）. However, pim-1 inhibitor II significantly （P 〈 0.05） abolished the protective effect of SP. Western blotting analysis demonstrated that, compared with I/R group, the expression of pim-1 and Bcl-2 in cytoplasm and mitochondria as well as the total p-Drplset637 in Sev group （P 〈 0.05） were upregulated. Meanwhile, SP inhibited Drp 1 compartmentalization to the mitochondria followed by a reduction in the release ofCyt C. Pretreatment with pim- 1 inhibitor II significantly （P 〈 0.05） abolished SP-induced pim- 1/p-Drp 1^ser637 signaling activation. Conclusions： These findings suggested that SP could attenuate myocardial ischemia-

关键词： Cardioprotection Ischemia-reperfusion Injury Mitochondria pim-1 Postconditioning Sevoflurane

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pim-1 as an artificial solid electrolyte interphase for stable lithium metal anode in high-performance batteries

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Journal of Energy Chemistry 2020年第3期29卷 83-90页

作者： Qiuli Yang Wenli Li Chen Dong Yuyan Ma Yuxin Yin Qibing Wu Zhitao Xu Wei Ma Cheng Fan Kening Sun Beijing Key Laboratory for Chemical Power Source and Green Catalysis School of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijing 100081China State Key Laboratory of Advanced Chemical Power Sources Guizhou Meiling Power Sources Co.Ltd.Zunyi 563003GuizhouChina

Lithium metal anode is a promising electrode with high theoretical specific capacity and low electrode potential.However,its unstable interface and low Coulombic efficiency,resulting from the dendritic growth of lithium,limits its commercial application.pim-1(pim:polymer of intrinsic microporosity),which is a polymer with abundant micropores,exhibits high rigidity and flexibility with contorted spirocenters in the backbone,and is an ideal candidate for artificial solid electrolyte interphases(SEI).In this work,a pim-1 membrane was synthesized and fabricated as a protective membrane on the surface of an electrode to facilitate the uniform flux of Li ions and act as a stable interface for the lithium plating/stripping process.Nodule-like lithium with rounded edges was observed under the pim-1 membrane.The Li@pim-1 electrode delivered a high average Coulombic efficiency(99.7%),excellent cyclability(80%capacity retention rate after 600 cycles at 1 C),and superior rate capability(125.3 m Ah g-1 at 10 C).Electrochemical impedance spectrum(EIS)showed that the pim-1 membrane could lower the diffusion rate of Li+significantly and change the rate-determining step from charge transfer to Li+diffusion.Thus,the pim-1 membrane is proven to act as an artificial SEI to facilitate uniform and stable deposition of lithium,in favor of obtaining a compact and dense Li-plating pattern.This work extends the application of pims in the field of lithium batteries and provides ideas for the construction of artificial SEI.

关键词： Lithium metal anodes Artificial solid electrolyte interphase pim-1 Diffusion-limited

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Inhibition of pim-1 attenuates the proliferation and migration in nasopharyngeal carcinoma cells

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Asian Pacific Journal of Tropical Medicine 2012年第8期5卷 645-650页

作者： Wei Jie Qi-Yi He Bo-Tao Luo Shao-Jiang Zheng Yue-Qiong Kong Han-Guo Jiang Ru-Jia Li Jun-Li Guo Zhi-Hua Shen Department of Pathology & Pathophysiology School of Basic Medicine ScienceGuangdong Medical College Hainan Provincial Key Laboratory of Tropical Medicine Hainan Medical College

Objective:To explore the role of proto-oncogene pim-1 in the proliferation and migration of nasopharyngeal carcinoma(NPC) cells.Methods:pim-1 expressions in NPC cell lines CNE1,CNE1-GL,CNE-2Z and C666-1 were examined by KT-PCR,western blotting and immunoflucesence,respectively.After CNE1,CNE1-GL and C666-1 cells were treated with different concentrations of pim-1 special inhibitor,quercelagetin,the cell viability,colony formation rate and migration ability were analyzed.Results:pim-1 expression was negative in well-differentiated CNE1 cells,whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells.Interestingly,CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of pim-1.Treatment of CNE1-GL and C666-1 cells with quercelagetin significantly decreased the cell viability,colony formation rate and migration ability but not the CNE1 cells.Conclusions:These findings suggest that pim-1 overexpression contributes to NPC proliferation and migration,and targeting pim-1 may be a potential treatment for anti-pim-1-expressed NPCs.

关键词： Nasopharyngeal carcinoma pim-1 Quercetagetin Cell proliferation Cell migration

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Recovery of free volume in pim-1 membranes through alcohol vapor treatment

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Frontiers of Chemical Science and Engineering 2021年第4期15卷 872-881页

作者： Faiz Almansour Monica Alberto Rupesh S.Bhavsar Xiaolei Fan Peter M.Budd Patricia Gorgojo Department of Chemical Engineering and Analytical Science School of EngineeringThe University of ManchesterManchester M139PLUK Department of Chemistry School of Natural SciencesThe University of ManchesterManchester M139PLUK

Physical aging is currently a major obstacle for the commercialization of pim-1 membranes for gas separation applications.A well-known approach to reversing physical aging effects of pim-1 membranes at laboratory scale is soaking them in lower alcohols,such as methanol and ethanol.However,this procedure does not seem applicable at industrial level,and other strategies must be investigated.In this work,a regeneration method with alcohol vapors(ethanol or methanol)was developed to recover permeability of aged pim-1 membranes,in comparison with the conventional soaking-in-liquid approach.The gas permeability and separation performance,before and post the regeneration methods,were assessed using a binary mixture of CO_(2) and CH_(4)(1:1,v:v).Our results show that an 8-hour methanol vapor treatment was sufficient to recover the original gas permeability,reaching a CO_(2) permeability>7000 barrer.

关键词： polymer of intrinsic microporosity(pim) pim-1 physical aging gas separation vapor-phase regeneration

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草鱼pim-1基因克隆、组织表达及多克隆抗体的制备

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江西农业大学学报 2022年第3期44卷 670-678页

作者：王辉刘林阮记明梁惜梅隗黎丽江西农业大学动物科学技术学院江西南昌330045

【目的】旨在进一步研究草鱼(Ctenopharygodon idella)莫洛尼鼠白血病病毒前病毒整合基因1(Proviral integration of moloney murineleukemia virus,pim-1)的功能。【方法】采用RACE法克隆草鱼pim-1(Cipim-1)的全长cDNA序列,并对其全长序列进行生物信息学分析。采用实时荧光定量PCR(qRT-PCR)检测Cipim-1在健康草鱼肝脏、肾脏、头肾、脾脏、肠、心脏、鳃、皮肤、肌肉及血液中的相对表达量,同时将Cipim-1基因(246~318 aa)片段克隆到原核表达质粒pGEX-4T-1中,转入E.coli Rosetta菌株,经IPTG诱导后成功表达重组蛋白pGEX-4T-1-pim1,随后采用纯化后的融合蛋白免疫新西兰大白兔获得抗血清,并通过ELISA测定抗血清效价,最后采用免疫印迹(Western Blotting)分析抗体的特异性。【结果】Cipim-1基因序列全长1082 bp,编码318个氨基酸,分子量为36.14 ku,具有1个蛋白激酶结构域(Protein kinase domain,35~287 aa)。同源性及系统进化结果显示,Cipim-1氨基酸序列与其它鱼类及哺乳类pim-1均具有较高的相似性和一致性,与斑马鱼(Danio rerio)pim-1亲缘关系最近。qRT-PCR结果表明,Cipim-1在草鱼血液和组织中均广泛表达,在心脏组织中的相对表达量最丰富,显著高于相对表达量最低的肝脏组织(P>0.05)。ELISA与Western blotting结果表明,经抗原亲和纯化后获得的Cipim-1多克隆抗体,效价高,能够特异性识别Cipim-1重组蛋白。【结论】研究克隆了Cipim-1基因,发现该基因在草鱼组织中广泛表达,且成功制备了Cipim-1多克隆抗体,为深入研究Cipim-1在草鱼细胞周期调控、增殖、分化及凋亡等生理过程中的作用奠定了基础。

关键词：草鱼 pim-1 克隆组织表达原核表达多克隆抗体

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Aerobic Oxidation of 2,3,6-Trimethylphenol with Reusable Homogenized Copper Catalysts

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Chemical Research in Chinese Universities 2021年第3期37卷 751-756页

作者： LI Meng WU Mingwei PAN Biwei XU Dandan LVSONG Zhuopu LI Dongqing ZHANG Fangrong HUANG Jun State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical EngineeringNanjing Tech UniversityNanjing211816P.R.China

The oxidation of 2,3,6-trimethylphenol(TMP)can produce both 2,3,5-trimethyl-1,4-benzoquinone(TMQ)and 2,3,5-trimethyl-1,4-hydroquinone(TMHQ)as important intermediates of vitamin E.With a soluble and porous polymer of intrinsic microporosity(pim-1),we developed a highly active and reusable CuO@pim-1 catalyst for the aerobic oxidation of TMP to TMQ and TMHQ.The CuO@pim-1 catalyst is high active and selective for the aerobic oxidation of TMP,and TMQ and TMHQ can be obtained in yields of 81%and 19%,respectively.The CuO@pim-1 catalyst has both advantages of homogeneous and heterogeneous and the CuO@pim-1 catalyst showed a higher activity than homogeneous(CuCl_(2))and heterogeneous(supported CuO)catalysts.Moreover,CuO@pim-1 catalyst can be recovered easily and reused at least 5 times without deactivation.

关键词： Aerobic oxidation Trimethylphenol Trimethylbenzoquinone Copper pim-1 CuO@pim-1

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pim-1活性位点调控蛋白功能的可视化研究

PIM-1活性位点调控蛋白功能的可视化研究

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作者：赵悠意大连理工大学

学位级别：硕士

pim-1蛋白,即莫罗尼小鼠白血病病毒前病毒整合位点（Proviral integration of MMLV,pim）蛋白激酶1,参与细胞生长、增殖、分化和迁移等功能变化和多种疾病的发生过程。pim-1蛋白结构和位点特性是这些功能的基础,但由于目前缺乏相应的活... 详细信息

pim-1蛋白,即莫罗尼小鼠白血病病毒前病毒整合位点（Proviral integration of MMLV,pim）蛋白激酶1,参与细胞生长、增殖、分化和迁移等功能变化和多种疾病的发生过程。pim-1蛋白结构和位点特性是这些功能的基础,但由于目前缺乏相应的活细胞研究手段,其活性位点与功能的关系不甚清楚。前期基于荧光共振能量转移（fluorescence resonance energy transfer,FRET）技术成功构建了在活细胞可视化检测pim-1活性的FRET探针EPHY,本文分别将其中pim-1序列的Lys67、Pro81/Asn82和Asp167位点进行负性突变,获得通过荧光可视化检测不同位点突变影响激酶分子功能的一系列FRET探针;通过细胞孵育实验和体外蛋白实验对运用这些探针研究蛋白位点的方法进行验证;之后通过施加pim-1激酶抑制剂、ATP以及磷酸肌醇3-激酶（phosphoinositide 3-kinase,PI3K）的特异性药物改变pim-1激酶活性,分析不同位点突变探针的FRET比率以及变化幅度差异,探索Lys67、Pro81/Asn82和Asp167位点参与pim-1激酶功能和PI3K/Akt/m TOR信号通路的作用。经过测序比对确认EPHY（K67M）、EPHY（P81S＿N82K）和EPHY（D167A）突变体探针构建成功;验证实验结果显示,EPHY及相应突变体探针能正确表征活性位点对pim-1激酶结构和功能的影响。EPHY（K67M）探针FRET比率显著上升,而在ATP和抑制剂作用下变化的幅度减小;EPHY（P81S＿N82K）探针FRET比率降低,同时在ATP作用下的FRET比率变化幅度也降低;EPHY（D167A）探针FRET比率显著下降,但此探针在药物刺激下的FRET比率变化幅度无明显改变。进一步发现,pim-1的K67M和P81S＿N82K突变不同程度削弱pim-1激酶的活性,K67M突变体的弱化最为明显,P81S＿N82K次之。本文构建的突变体探针为研究pim-1蛋白几个不同活性位点的功能变化提供了可视化检测工具。Lys67位点与pim-1激酶的底物结合和催化作用相关,直接调控PI3K/Akt/m TOR信号通路;Pro81/Asn82位点参与调节pim-1蛋白与底物的结合度,同时参与pim-1激酶的磷酸化调控,从而影响pim-1激酶与PI3K/Akt/m TOR通路的相互作用;Asp167位点影响pim-1激酶与底物复合物的结构,但对激酶功能发挥无显著作用。本文基于FRET生物探针技术建立了在活细胞内探究pim-1蛋白活性位点影响激酶功能和信号分子调控机制的可视化研究方法,可以促进pim-1激酶所在信号通路上下游效应的机制探究,也为靶向pim-1激酶的药物筛选开发和疾病治疗提供启发。

关键词： FRET 生物探针 pim-1 活性位点 PI3K/Akt/mTOR

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基于氰基转化的自愈合超疏水pim-1复合膜制备与性能研究

基于氰基转化的自愈合超疏水PIM-1复合膜制备与性能研究

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作者：彭云飞青岛大学

学位级别：硕士

2020年“两会”期间,国家提出“碳达峰”、“碳中和”的“双碳”目标,将CO捕集、封存和利用(CCUS)技术再次推向新的研究高潮。其中,膜分离技术具有投资少、节能、操作简单和不发生相变等优点,是CO捕集更有效的方法。固有微孔聚合物pim-... 详细信息

2020年“两会”期间,国家提出“碳达峰”、“碳中和”的“双碳”目标,将CO捕集、封存和利用(CCUS)技术再次推向新的研究高潮。其中,膜分离技术具有投资少、节能、操作简单和不发生相变等优点,是CO捕集更有效的方法。固有微孔聚合物pim-1是近年来发展的一类具有高渗透性及中等选择性的聚合物材料,由于其较高的自由体积分数、优异理化稳定性以及特有的CO分离性,有望成为新一代商用气体分离膜。但是,pim-1膜的气体选择性和渗透性无法满足实际工业生产要求:(1)pim-1的羧酸化改性作为提高气体选择性的有效方法,存在转化过程复杂,效率低下以及成膜特性差的研究难点;(2)液态水会占用pim-1膜的气体传输孔道,降低膜气体渗透性能。本文针对以上问题展开研究:(1)结合实验目标,对所需材料进行制备和表征。在前人研究基础上,通过改变纯化过程中THF的量,实现对pim-1分子量的调控;采用研磨、搅拌、加压以及升温等方法提高pim-1的羧酸化转化效率;设计静电纺丝和化学气相沉积方案,完成PVDF/GO/POTS@pim-1复合膜的制备;通过各种化学和物理表征方法,对材料的理化性能进行测试。以上材料制备和表征过程为下文机理的总结以及性能的分析奠定良好的基础。(2)使用水热法对pim-1进行羧酸化改性(C-pim),该方法实现在10 h内羧酸转化率达到90%以上,转化效率是已有最高效率的近5倍,且保留了成膜特性。所制备的C-pim膜的CO/CH选择性为35.8,H/N选择性为48.28,均超过了2008年的罗伯逊上限。利用分子结构分析,XRD、GPC、N吸附/解吸,CO吸附/解吸等测试手段,基于吸附—扩散模型,从微观到宏观表征得出结论:C-pim膜能够同时提高对CO等气体的吸附选择性和扩散选择性。(3)利用静电纺丝技术和化学气相沉积技术在pim-1膜表面制备超疏水复合层(PVDF/GO/POTS@pim-1)。所制备的超疏水层与pim-1膜展现出良好的连接性,水接触角达到152.75±0.5°。该表面在化学腐蚀(p H=1-14)、水冲击(P=15.9k Pa)以及热水处理(70℃)等条件下均能保持超疏水性,同时还展现出一定的超疏水自愈合性能。该pim-1复合膜在30 min内的吸水率从69%降低到12%,同时保持了pim-1膜原有的高气体渗透性。本文成功制备出高气体选择性的C-pim膜和具有超疏水自愈合性能的PVDF/GO/POTS@pim-1复合膜,这大幅度提升了pim-1改性膜的制备效率以及应用于复杂工况条件下的适应能力,提高了CO等气体的分离、捕获能力,为实现国家的“双碳”目标提供了新的解决方案。

关键词： pim-1 二氧化碳气体分离超疏水性自愈合

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