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Are type IV pili involved in Vibrio anguillarumvirulence towards sea bass (Dicentrarchus labraxL.) larvae?

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Agricultural Sciences 2013年第6期4卷 30-38页

作者： Ingeborg Frans Pieter Busschaert Kristof Dierckens Chris W. Michiels Kris A. Willems Bart Lievens Peter Bossier Hans Rediers Centre for Food and Microbial Technology M2S KU Leuven Leuven Belgium Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM) Thomas More Mechelen Campus De Nayer Department of Microbial and Molecular Systems (M2S) KU Leuven Association Sint-Katelijne-Waver Belgium Scientia Terrae Research Institute Sint-Katelijne-Waver Belgium Laboratory for Process Microbial Ecology and Bioinspirational Management (PME&BIM) Thomas More Mechelen Campus De Nayer Department of Microbial and Molecular Systems (M2S) KU Leuven Association Sint-Katelijne-Waver Belgium Scientia Terrae Research Institute Sint-Katelijne-Waver Belgium Centre for Food and Microbial Technology M2S KU Leuven Leuven Belgium Laboratory of Aquaculture & Artemia Reference Center Department of Animal Production Ghent University Ghent Belgium Laboratory of Aquaculture & Artemia Reference Center Department of Animal Production Ghent University Ghent Belgium

Vibrio anguillarum, an important bacterial fish pathogen, expresses a variety of virulence factors contributing to its ability to cause vibriosis in fish. Many virulence factors of this pathogen remain however unknown. For example, a type IV pilus system was previously reported to be potentially involved in the virulence of this bacterium but no experimental evidence was reported yet. In this study, complete genome sequencing of V. anguillarum strain VIB15, shown to be highly virulent towards sea bass (Dicen- trarchus labrax L.) larvae, revealed the presence of a PilA pilin. A V. anguillarum VIB15 pilA mutant was constructed and the pathogenicity of this mutant was assessed in a gnotobiotic sea bass system developed for virulence screening. Our results suggest that the V. anguillarum pilA gene is not crucial for virulence towards sea bass larvae. Possibly, another type IV pilus system identified in V. anguillarum, showing homology to the mannose-sensitive hemagglutinin pilin of Vibrio cholerae, may complement the pilA mutation. Alternatively, the type IV pilus system has a role in infection of juvenile or adult fish, rather than in the larval phase. As such, further research is required to unravel the potential role of type IV pili in V. anguillarum virulence.

关键词： Virulence Vibrio anguillarum Fish Pathogen Type IV pili

Study on the mechanism of Porphyromonas gingivalis pili mediated ather osclerosis

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Journal of Hainan Medical University 2020年第18期26卷 69-72页

作者： Zhu-Ling Guo Sha-Sha Liu Yu Zhang Qi-Ya Fu School of Stomatology Hainan Medical UniversityHaikou 570100PR China Department of Stomatology the First Affiliated Hospital of Hainan Medical UniversityHaikou 570102PR China

Chronic periodontitis is the most common infectious periodontal disease caused by plaque *** is an important bacterial component of Porphyromonas *** studies have shown that chronic periodontitis can increase the risk of cardiovascular disease,and atherosclerosis is the pathological basis of cardiovascular *** article will summarize the mechanism of Porphyromonas gingivalis pili protein mediated chronic periodontitis and atherosclerosis,in order to expand new ideas of disease prevention and treatment.

关键词： Porphyromonas gingivalis pili Chronic periodontitis Atherosclerosis

Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley,Malaysia

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Asian Pacific Journal of Tropical Medicine 2023年第2期16卷 80-88页

作者： Nurul Diana Dzaraly Mohd Nasir Mohd Desa AbdulRahman Muthanna Siti Norbaya Masri Niazlin Mohd Taib Nurul Hana Zainal Baharin Nurshahira Sulaiman Nurul Asyikin Abdul Rahman Navindra Kumari Palanisamy Zarizal Suhaili Semiat Opeyemi Yusuff Nor Iza A.Rahman Tuan Suhaila Tuan Soh Fatimah Haslina Abdullah Department of Biomedical Sciences Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaUPM SerdangSelangorMalaysia Department of Medical Microbiology Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaUPM SerdangSelangorMalaysia School of Biology Faculty of Applied SciencesUniversiti Teknologi MARA Negeri SembilanKuala Pilah BranchKuala PilahNegeri SembilanMalaysia Department of Medical Microbiology and Parasitology Faculty of MedicineUniversiti Teknologi MARASungai Buloh CampusJalan HospitalSungai BulohSelangorMalaysia School of Animal Science Aquatic Science and EnvironmentFaculty of Bioresources and Food IndustryUniversiti Sultan Zainal AbidinBesut CampusBesutTerengganuMalaysia Faculty of Medicine Universiti Sultan Zainal AbidinJalan Sultan MahmudKuala TerengganuTerengganuMalaysia Department of Pathology Sungai Buloh HospitalJalan HospitalSungai BulohSelangorMalaysia Department of Pathology Sultanah Nur Zahirah HospitalJalan Sultan MahmudKuala TerengganuTerengganuMalaysia

Objective:To characterise a collection of pili-carrying and none pili-carrying pneumococcal isolates of clinical origin for serotypes,antibiotic resistance and ***:In total,42 clinical isolates were collected between October 2017 and December *** isolates were analysed for antimicrobial susceptibility,serotype distribution,detection of pneumococcal virulence and pilus *** sequence typing was performed only for piliated isolates,followed by phylogenetic ***:The common isolation sites among the pneumococcal isolates were tracheal aspirate(28.6%),blood(26.2%),and sputum(23.8%).Fifty percent isolates were resistant to erythromycin,tetracycline(50.0%)and trimethoprim-sulfamethoxazole(43.0%).The most frequent were serotypes 19F(28.6%),6A/B(23.8%)and 19A(14.3%).piliated isolates were detected in a small proportion(33.3%);64.3%were ***320 was the prevalent sequence type among the piliated isolates and genetically related to the Pneumococcal Molecular Epidemiology Network clones Taiwan19F-14(CC271).In the phylogenetic analysis,some piliated isolates showed a close association having similar ST320,carrying serotype 19A and both pilus genes indicating their clonal ***:Pneumococcal lineages of piliated isolates have been globally disseminated and pili could have played a role in the spread of antibiotic resistant clones.

关键词： Pneumococci pili Genotype Malaysia

迟缓爱德华菌菌毛蛋白原核表达及多抗血清制备

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中国兽医学报 2017年第9期37卷 1705-1709页

作者：张志强杨楠李永慧潘晓艺单晓枫康元环焦贺静韩红升吴同垒高桂生河北科技师范学院河北省预防兽医学重点实验室河北秦皇岛066004 秦皇岛第二医院河北昌黎066600 浙江省淡水水产研究所浙江湖州214000 吉林农业大学动物科学技术学院吉林长春130000 秦皇岛粮丰海洋生态科技开发股份有限公司河北秦皇岛066004

为研究迟缓爱德华菌菌毛蛋白pili的功能和免疫原性,本研究利用PCR方法从迟缓爱德华菌基因组中克隆出pili基因,并将其构建到pET-32a原核表达载体上,重组载体转化BL21大肠杆菌诱导重组蛋白表达,通过SDSPAGE和Western blot方法验证重组蛋... 详细信息

为研究迟缓爱德华菌菌毛蛋白pili的功能和免疫原性,本研究利用PCR方法从迟缓爱德华菌基因组中克隆出pili基因,并将其构建到pET-32a原核表达载体上,重组载体转化BL21大肠杆菌诱导重组蛋白表达,通过SDSPAGE和Western blot方法验证重组蛋白表达。用亲和层析方法纯化蛋白,纯化的蛋白免疫小鼠,所得的多抗血清能够特异性识别迟缓爱德华菌pili蛋白,为研究pili蛋白奠定基础。

关键词：迟缓爱德华菌菌毛蛋白 pili 蛋白表达纯化

Adhesion of Gallibacterium anatisto Chicken Oropharyngeal Epithelial Cells and the Identification of Putative Fimbriae

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Advances in Microbiology 2012年第4期2卷 505-510页

作者： Mónica L. Salgado Lucio Sergio Vaca Candelario Vázquez Edgar Zenteno Ismael Rea Víctor M. Pérez-Márquez Erasmo Negrete-Abascal Biotecnología Veterinaria Tehuacán Mexico Carrera de Biología Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México (UNAM) Tlalnepantla México Centro de Investigaciones en Ciencias Microbiológicas Benemérita Universidad Autónoma de Puebla (BUAP) Puebla México Departamento de Bioquímica Facultad de Medicina Universidad Nacional Autónoma de México México City México

Microbial infections are typically initiated by the colonization of tissues by a specific mechanism that promotes adherence to host cells or tissues. In this work, we characterized the ability of Gallibacterium anatis F149T to express fimbriae that may be involved in mucosal attachment. Using transmission electron microscopy, the fimbriae-like structures could be observed on the surface of negatively stained G. anatis F149T, and these structures were further visualized after being released by physical shaking. When the fimbriae-like structures were separated by SDS-PAGE, the proteins comprising them were isolated and sized at 13 and 25 kDa. G. anatis F149T was able to adhere to chicken oropharyngeal epithelial cells. Adhesion could be completely inhibited by pretreatment of the bacterial cells with trypsin, whereas 25% inhibition was attained after pretreatment with an antiserum against the 13 kDa protein. We demonstrated by immuno-gold electron microscopy that the antibodies from the antiserum were specifically associated with the fimbria-like structures on G. anatis. These results indicated that G. anatis F149T expresses fimbriae that contribute to its adhesion to chicken oropharyngeal epithelial cells and may be important for colonization of the upper respiratory tract.

关键词： G. anatis Adhesion Fimbria pili

Regulation of fim genes in uropathogenic Escherichia coli

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World Journal of Clinical Infectious Diseases 2011年第1期1卷 17-25页

作者： William R Schwan Department of Microbiology University of Wisconsin-La Crosse1725 State StreetLa CrosseWI 54601United States

Uropathogenic Escherichia coli(UPEC)is the leading cause of urinary tract infections in women,causing significant morbidity and mortality in this *** to host epithelial cells is a pivotal step in the pathogenesis of *** of the most important virulence factors involved in mediating this attachment is the type 1 pilus(type 1 fimbria)encoded by a set of fim genes arranged in an *** expression of type 1 pili is controlled by a phenomenon known as phase variation,which reversibly switches between the expression of type 1 pili(Phase-ON)and loss of expression(Phase-OFF).Phase-ON cells have the promoter for the fimA structural gene on an invertible DNA element called fimS,which lines up to allow transcription,whereas transcription of the structural gene is silenced in Phase-OFF *** orientation of the fimS invertible element is controlled by two site-specific recombinases,FimB and *** conditions cause transcriptional and post-transcriptional changes in UPEC cells that affect the level of regulatory proteins,which in turn play vital roles in modulating this phase switching *** role of fim gene regulation in UPEC pathogenesis will be discussed.

关键词： Type 1 fimbriae Type 1 pili Gene regulation Uropathogenic Escherichia coli Urinary tract

PCR Detection of Pilus-associated Genes and Serotype Identification of Pathogenic Escherichia coli Isolated from Chickens in the Jidong Area

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Agricultural Biotechnology 2016年第2期5卷 43-45页

作者： Qiumei SHI Yanying ZHANG Xiumin WANG Baoxin YANG Zhiqiang ZHANG Xiaojiao Hou Xinhua SHAO Xia MENG Yang YANG Guoqiang ZHU Key Laboratory of Preventive Veterinary Medicine of Hebei Province/Hebei Normal University of Science and Technology Changli 066600China Beijing Centre Biology Co. Ltd.Beijing 102600China Hebei Xinhua Keji Pharmaceutical(Group)Corporation Luancheng 051430China Yangzhou University Yangzhou 225009China

[ Objective] This study aimed to explore the presence of type I pill （fimC gene） and P pill （papC gene） and identify the serotype of pathogenic E. coli isolated from chickens in the Jidong Area. [ Method] Type I pill （fimC gene） and P pill （papC gene） were detected by PCR. The serotype was identified by con- ventional agglutination test. [ Result ] The results showed that 100% of chicken-derived E. coil strains expressed type I pill （fimC gene） ; 39.1% （9/23） of chick- en-derived E. coli strains expressed P pill （papC gene）. In addition, 23 isolates of chicken-derived E. coil were assigned to 14 O serotypes, including O78, O93, O 45, O101, O38, O88, O24, O1, O163, O53, O15, O87, O34 and O29, among which O78 was the dominant serotype that accounted for 42.8% （6/14） of the total strain number. [ Conclusion] Chicken-derived E. coli strains in the Jidong Area belonged to 14 serotypes, and 078 was the dominant serotype; 83.3% of 078 serotvDe E. coli strains expressed both tvDe I Dill and P Dill.

关键词： Chicken Escherichia coli Serotype pili