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Assessment of phytotoxic, genotoxic and enzyme inhibition potential of Sterculia diversifolia

Journal of Traditional Chinese Medicine 2022年第4期42卷 595-603页

作者： Fazle Rabbi Amir Zada Amna Nisar Department of Pharmacy University of PeshawarPeshawar 25120Khyber PakhtunkhwaPakistan Department of Pharmacy Abasyn University PeshawarPeshawar25000Khyber PakhtunkhwaPakistan.fazle.rabbi@abasyn.edu.pk Telephone:+92-0314-9244566

OBJECTIVE:To evaluate Sterculia diversifolia stem bark and leaves for phytotoxic,genotoxic and enzymes inhibition potential.METHODS:phytotoxic activity of both stem bark and leaves were screened using Lemna minor.The genotoxic activity of Sterculia diversifolia stem bark and leaves extracts were tested using comet assay protocol while enzyme inhibition activity of crude extract and various fractions of both stem bark and leaves were evaluated using acetyl cholinesterase,lipoxygenase,β-glucuronidase,urease,xanthine oxidase and carbonic anhydrase.RESULTS:phytotoxic activity showed significant results in dose dependant manner in both stem bark(ethyl acetate and n-butanol)and leaves(ethyl acetate,nbutanol and n-hexane)fractions.In genotoxic activity,dichloromethane fraction showed significant activity followed by ethyl acetate fraction.Acetyl cholinesterease inhibitory activity showed significant results in both stem bark and leaves fractions,while significant lipoxygenase inhibition was shown by ethyl acetate,dichloromethane,crude extract and n-hexane fractions of both stem bark and leaves.β-glucuronidase,urease and carbonic anhydrase inhibitory activity showed highly significant results in ethyl acetate fraction of both stem bark and leaves,while xanthine oxidase inhibition was shown by dichloromethane fraction of stem bark and leaves extracts.CONCLUSIONS:This study emphasizes the important phytotoxic,genotoxic and enzyme inhibition effects of Sterculia diversifolia stem bark and leaves.Hence,it is clear that Sterculia diversifolia stem bark and leaves possess phytotoxic,genotoxic and enzyme inhibitory agents.

关键词： Sterculia plant extracts phytotoxic mutagenicity tests enzymes

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Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus brutia and Pinus pinea resin essential oils

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Chinese Journal of Natural Medicines 2014年第12期12卷 901-910页

作者： ULUKANLI Zeynep KARABRKLü Salih BOZOK Fuat ATES Burhan ERDOGAN Selim CENET Menderes KARAASLAN Merve Gksin Smaniye Korkut Ata University Faculty of Science and Arts Department of Biology ?n?nü University Faculty of Science Department of Chemistry

Essential oils of the resins of Pinus brutia and Pinus pinea were evaluated for their biological potential. Essential oils were characterized using GC-MS and GC/FID. In vitro antimicrobial, phytotoxic, antioxidant, and insecticidal activities were carried out using the direct contact and the fumigant assays, respectively. The chemical profile of the essential oils of the resins of P. pinea and P. brutia included mainly α-pinene(21.39% and 25.40%), β-pinene(9.68% and 9.69%), and caryophyllene(9.12% and 4.81%). The essential oils of P. pinea and P. brutia exerted notable antimicrobial activities on Micrococcus luteus and Bacillus subtilis, insecticidal activities on Ephestia kuehniella eggs, phytotoxic activities on Lactuca sativa, Lepidium sativum, and Portulaca oleracea, as well as antioxidant potential. Indications of the biological activities of the essential oils suggest their use in the formulation of ecofriendly and biocompatible pharmaceuticals.

关键词： Essential oil Antimicrobial Insecticidal phytotoxic Antioxidant Pinus brutia Pinus pinea

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Antimicrobial,hemagglutination and phytotoxic activity of crude ethanolic and aqueous extracts of Seriphidium kurramense

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Journal of Traditional Chinese Medicine 2018年第3期38卷 433-438页

作者： Kafeel Ahmad Amjad Ali Waqar Ahmed Afridi Ramla Somayya Muhammad Jawad Ullah Centre of Biotechnology and Microbiology University of Peshawar

OBJECTIVE: To investigate the antimicrobial activity, hemagglutination and phytotoxic activity of crude ethanolic and aqueous extracts of Seriphidium kurramense.METHODS: The extracts were analyzed by agar well diffusion assays against five bacterial species:Staphylococcus aureus(S. aureus), methicillin-resistant S. aureus, Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis, and Salmonella typhi. The extracts were also screened against six fungal species — Aspergillus niger, Aspergillus flavus, Alternaria solani, Rhizoctonia solani, Fusarium solani and Pleurotus florida — using the agar tube diffusion method. Additionally, hemagglutination and phytotoxic activities of the crude ethanolic and aqueous extracts were assessed.RESULTS: The crude ethanolic and aqueous extracts showed dose-dependent inhibition of the various tested fungal and bacterial strains. No hemagglutination activity was observed. Both the ethanolic and aqueous extracts showed dose-dependent phytotoxic activity toward Lemna minor.CONCLUSION: The crude ethanolic and aqueous extracts of Seriphidium kurramense possess good antimicrobial and phytotoxic activities, but no hemagglutination activity.

关键词： Anti-bacterial agents Antifungal agents phytotoxic Seriphidium kurramense

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In vitro biological screening of the stem of Desmodium elegans

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Asian Pacific Journal of Tropical Biomedicine 2013年第9期3卷 711-715页

作者： Arshad Khan Rabia Usman Ming-Liang Wang Abdur Rauf Naveed Muhammad Akhatar Aman Taha Hussein Musa Tahir School of Chemistry and Chemical Engineering Southeast University Institute of Chemical Sciences University of Peshawar Department of Pharmacy Hazara University School of Public Health Southeast University Nanjing

Objective:To explore the medicinal importance of the stem of Desmodium elegans,methanolic extract,and its different solvent fractions were evaluated for brine shrimp lethality,insecticidal and phytotoxicity,antifungal,and antibacterial activities.Methods:The methanolic extract and its solvent fractions were tested for cytotoxic,phytotoxic,insecticidal,antifungal,and antibacterial effects using our previous published protocols.Results:The methanolic,DCM,ethyl acetate and n-butanol fractions exhibited insecticidal effect against Callosobruchus analis and Rhyzopertha dominie.The methanolic extract,n-hexane,DCM ethyl acetate and n-butanol showed 75,85,85,65 and 5%phytotoxicity at the tested concentration of 500μg/ml.respectively.The solvent fractions(DCM and ethyl acetate)were effective against F.solani(10%and 20%,inhibition respectively).All the tested samples were devoid of cytotoxic and antibacterial effects.Conclusions:It was concluded diat diis plant can be practiced for control of weeds and insects.

关键词： Leguminosae Desmodium elegans Brine shrimp cytotoxicity phytotoxic Insectioidal and antimicrobial activities

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Sensitivity of Barley Varieties to Aluminum Ions: Separately Effects and Combine with Iron Ions

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American Journal of Plant Sciences 2013年第12期4卷 49-52页

作者： Boris I. Synzynys Natalia V. Amosova Liliia N. Ulyanenko All-Russia Institute of Plant Protection St. Peterburg-Pushkin Russia Department of Ecology State Technical University of Nuclear Power En-gineering Obninsk Russia

Differences in the barley varieties have been revealed from tolerance to iron (Fe) and aluminum (Al) ions as well as to their combined effect. Received results allowed to separate barley variety into some (three) groups: the first—Al-tolerant varieties, the second—Al-sensitive ones and third—moderately resistant variety. The increased concentration of Fe had practically no effect on biometric (seed germination energy) and cytogenetics (frequency of chromosome aberrations and mitotic index) parameters as compared to the reference values. At the same time, iron ion significantly reduces the phytotoxic effect for Al-tolerant varieties in case of these elements jointly presented in solution.

关键词： Barley Varieties Aluminum and Iron Ions phytotoxic Effect

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