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Development of 3D bioprinting:From printing methods to biomedical applications

Asian Journal of Pharmaceutical Sciences 2020年第5期15卷 529-557页

作者： Zeming Gu Jianzhong Fu Hui Lin Yong He State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province School of Mechanical EngineeringZhejiang UniversityHangzhou 310027China Department of General Surgery Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310058China

Biomanufacturing of tissues/organs in vitro is our big dream,driven by two needs:organ transplantation and accurate tissue *** the last decades,3D bioprinting has been widely applied in the construction of many tissues/organs such as skins,vessels,hearts,etc.,which can not only lay a foundation for the grand goal of organ replacement,but also be served as in vitro models committed to pharmacokinetics,drug screening and so *** organs are so complicated,many bioprinting methods are exploited to figure out the challenges of different *** the question is how to choose the suitable bioprinting method?Herein,we systematically review the evolution,process and classification of 3D bioprinting with an emphasis on the fundamental printing principles and commercialized *** summarize and classify extrusion-based,dropletbased,and photocuring-based bioprinting methods and give some advices for *** them,coaxial and multi-material bioprinting are highlighted and basic principles of designing bioinks are also discussed.

关键词： 3D bioprinting Extrusion-based bioprinting Droplet-based bioprinting photocuring-based bioprinting Bioink

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