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Effects of Shuanghuangbu on the total protein content and ultrastructure in cultured human periodontal ligament cells

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Chinese Medical Journal 2004年第11期117卷 1693-1696页

作者：许彦枝邹慧儒王小玲刘世正王永军 Department of Stomatology The Fourth Hospital Hebei Medical University Shijiazhuang 050011 China

Background Successful periodontal regeneration depends on the migration, proliferation and differentiation of periodontal ligament cells in periodontal defects. The total protein content and the ultrastructure demonstrate the metabolizability and activity of periodontal ligament cells. This study was conducted to observe the effects of Shuanghuangbu, a mixture of medicin al herbs, on the total protein content and the ultrastructure of human periodontal ligament cells. Methods periodontal ligament cells were grown to confluence and then cultured in Dulbecco’s modified eagle medium (DMEM) supplemented with Shuanghuangbu over the concentration range of 0 to 1000 μg/ml. The total protein content in cultured cells was determined by using Coommasie brilliant blue technique. periodontal ligament cells were incubated in 0 and 100 μg/ml Shuanghuangbu decoction for 5 days, then observed through transmission electron *** The total protein content of human periodontal ligament cells increased in each experiment group added 10-1000 μg/ml Shuanghuangbu respectively, and the effect of 100 μg/ml was excellent. Under the transmission electron microscope, there were more rough endoplasmic reticulums and mitochodrias in the experiment group than those in the control group. Conclusion Shuanghuangbu stimulates the protein synthesis of human periodontal ligament cells and improves human periodontal ligament cells’ metabolizability and activity.

关键词： periodontal ligament cells protein synthesis Rhizoma coptidis Radix scutellariae Rhizoma drynariac

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Effects of Apatite Nanocrystals on Proliferation, Subcellular Structure, Protein Synthesis Activity of Human periodontal ligament cells

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Journal of Wuhan University of Technology(Materials Science) 2005年第B12期20卷 102-104页

作者：聂蓉蓉莫安春 West China College of Stomatology Sichuan University Chengdu 610041 China

To determine the cellular events occurring in the presence of yttrtum/ hyclroxyapatite（Y / HA ） or HA nanocrystals, human periodontal ligament （HPDL） cells were isolated and maintained in culture. The specificity of the cells was evidenced by their proliferation, subcellular structure, and deposition of extracellular matrix components. The presence of nanocrystals was significantly related to an increase in the proliferation. Moreover, the presence of Y/HA nanocrystals was significantly related to an increase in the proliferation with HA nanocrystals. Transmission electron microscopy （TEM） demonstrated the phagocytotic process of HPDL cells toward Y/ HA or HA nanocrystuls . The presence of Y/ HA or HA nanocrystuls was significantly related to an increase in the protein synthesis activity of HPDL cells.

关键词： yttrium hydroxyapatite nanocrystals periodontal ligament cells

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A novel porcine acellular dermal matrix scaffold used in periodontal regeneration

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International Journal of Oral Science 2013年第1期5卷 37-43页

作者： Jing Guo Hui Chen Ying Wang Cheng-Bo Cao Guo-Qiang Guan Shandong Provincial Key Laboratory of Oral Biomedicine Stomatology Hospital of Shandong University Department of Orthodontics School of Dental MedicineUniversity at BuffaloState University of New York School of Chemistry and Chemical Engineering Shandong University

Regeneration of periodontal tissue is the most promising method for restoring periodontal *** find a suitable bioactive three- dimensional scaffold promoting cell proliferation and differentiation is critical in periodontal tissue *** objective of this study was to evaluate the biocompatibility of a novel porcine acellular dermal matrix as periodontal tissue scaffolds both in vitro and in *** scaffolds in this study were purified porcine acellular dermal matrix(PADM) and hydroxyapatite-treated PADM(HA-PADM). The biodegradation patterns of the scaffolds were evaluated in *** biocompatibility of the scaffolds in vivo was assessed by implanting them into the sacrospinal muscle of 20 New Zealand white *** hPDL cells were cultured with PADM or HA-PADM scaffolds for 3,7,14,21 and 28 *** viability assay,scanning electron microscopy(SEM),hematoxylin and eosin(H&E) staining, immunohistochemistry and confocal microscopy were used to evaluate the biocompatibility of the *** vitro,both PADM and HA-PADM scaffolds displayed appropriate biodegradation pattern,and also,demonstrated favorable tissue compatibility without tissue necrosis,fibrosis and other abnormal *** absorbance readings of the WST-1 assay were increased with the time course, suggesting the cell proliferation in the *** hPDL cells attaching,spreading and morphology on the surface of the scaffold were visualized by SEM,H&E staining,immnuohjstochemistry and confocal microscopy,demonstrated that hPDL cells were able to grow into the HA-PADM scaffolds and the amount of cells were growing up in the course of *** study proved that HA-PADM scaffold had good biocompatibility in animals in vivo and appropriate biodegrading characteristics in *** hPDL cells were able to proliferate and migrate into the *** observations may suggest that HA-PADM scaffold is a potential cell carrier for periodontal tissue regeneration.

关键词： periodontal ligament cells periodontal regeneration porcine acellular dermal matrix scaffold tissue engineering

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Metformin ameliorates HMGB1-mediated oxidative stress through mTOR pathway in experimental periodontitis

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Genes & Diseases 2023年第2期10卷 542-553页

作者： Boyang Sun Siqi Ying Qian Ma Han Li Jie Li Jinlin Song College of Stomatology Chongqing Medical UniversityChongqing 400016China Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing 400016China Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing 400016China

Periodontitis is an oral chronic inflammatory *** tissue destruction and promoting tissue regeneration are important means for the treatment of *** not only has hypoglycemic effect but also has anti-inflammatory *** has been shown to inhibit oxidative stress and activate autophagy through AMPK/mTOR *** mobility group box 1(HMGB1)has been implicated in the pathogenesis of many inflammatory diseases including periodontitis,it can participate in the induction of oxidative ***1 is an autophagy regulator under oxidative stress,which can activate mTOR ***,it is not clear whether metformin is related to HMGB1 and its mechanism in the process of *** viability and expression of inflammatory cytokines were clarified by Cell Counting Kit-8,real-time PCR and enzyme-linked immunosorbent *** blot and immunofluorescence were conducted to determine HMGB1 intracellular localization and expression of autophagy-associated proteins in *** periodontitis mice model was induced by administering a *** was performed to detect the expression and localization of HMGB1 in *** results of CCK-8,real-time PCR,enzyme-linked immunosorbent assay,Western blot and immunofluorescence showed lipopolysaccharide(LPS)treatment inhibited cell viability,and increased HMGB1 expression at a dose-independent *** can reduce the effect of *** also improves autophagy pathway inhibited by LPS and down-regulates mTOR *** addition,metformin attenuated alveolar bone resorption induced by *** study provides new evidence for that metformin is a potential drug for the treatment of periodontitis and HMGB1 may be a potential target for periodontal intervention.

关键词： Autophagy Metformin Oxidative stress periodontal ligament cells Periodontitis

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Effect of Complex Biomembranes in repairing periodontal tissue defect

Effect of Complex Biomembranes in repairing periodontal tiss...

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中华口腔医学会第14次全国口腔医学学术会议（2012年会）

作者：林泓兵吉林大学口腔医学院

Objective:The ideal therapy of periodontitis is to rebuild periodontal tissue physiological structure and *** recent years,studies of periodontal therapy using tissue engineering offer new ideas for realizing it. Tissue engineering includes three aspects,this study mainly chose periodontal ligament cells(PDLCs),collagen(Co),poly lactic acid/gather hydroxy-acetic acid cpolymers(PLGA)and platelet-rich plasma(PRP).PDLCs are the basic of periodontal tissue *** and Co have good biocompatibility,are often referred to as *** contains lots of platelet growth factors,which can effectively promote periodontal tissue regeneration. Methods:Synthesized PLGA membrane(0.5 mm thickness,5×2 mm), compounded with PDLCs in vitro for 3 *** Co(0.30-0.69 mm thickness) into 7×4 *** *** divided 60 adult male Wistar rats(6-8 weeks,160g-180g weight)into 5 groups,made mandible alveolar bone defect models(5×2×1 mm),Group A was control,Group B was PLGA+Co,Group C was PLGA+PRP+Co,Group D was PLGA+PDLCs+Co,Group E was PLGA+PDLCs+ PRP+*** 4 rats of each group to death at 2rd,4th and 6th week after operation,H-E dyed,observed the repairing effect of periodontal tissue defect. Results:The qualities of new bone formation of Group B,C,D and E were more than Group A,that of Group E were more than Group C and D,that of Group C and D were more than Group B,all of above had statistics meanings. Comparison between Group C and D had no statistically significance. Conclusions:PLGA+PDLCs+PRP+Co played significant role in promoting periodontal tissue growth,better than used PDLCs,scaffold and PRP sepatately.

关键词： periodontal ligament cells collagen polylactic-co-glycolic acid platelet-rich plasma periodontal tissue regeneration

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