检索结果-南通市图书馆

Chinese Journal of Oceanology and Limnology 2012年第3期30卷 471-475页

作者：崔玉琳姜鹏王金锋李富超陈英杰郑国庭秦松 Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciences Graduate University of Chinese Academy of Sciences Key Laboratory of Marine Biotechnology Ningbo University

Platymonas (Tetraselmis) subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The bar gene is a practicable and selectable marker gene. The vector containing the expression cassette of the bar gene was transferred to P. subcordiformis by both particle bombardment and glass-bead agitation and transformants were then selected using Basta. Finally, Southern blotting analysis indicated that the bar gene had been successfully integrated into the nuclear genome of P. subcordiformis using both of the transgenic techniques, with the transformation efficiency of the glass-bead method being slightly higher than that of particle bombardment. This is the first report on stable transformation of P. subcordiformis, and will improve fundamental research and enlarge application of this alga.

关键词： bar glass-bead agitation nuclear transformation particle bombardment Platymonas subcordiformis

Transfer and Detection of barstar Gene to Maize Inbred Line 18-599 (White) by particle bombardment

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2007年第6期6卷 652-656页

作者： SUN Qing-quan ZHANG Ying RONG Ting-zhao DONG Shu-ting ZUO Zhen-peng Shandong Agricultural University/State Key Laboratory of Crop Biology Taian 271018 P.R.China Sichuan Agricultural University Yaan 625014 P.R.China

In China, the purity of maize hybrid strain is discomforting to the development of seed industrialization. Finding a new method for reproduction of maize hybrid strain is necessary. In this study, using particle bombardment, barstar gene was transferred into maize inbred line 18-599 （White）, which is an antiviral and high quality maize inbred line. By molecular detection of the anther of transgenic maize, two plants transferred with barstar gene were gained in this study, which are two restorer lines. The two plants showed normal male spike, and lively microspores. But the capacity of the two restorer lines should be studied in the future. The aim of this study is to find a new method of reproduction of maize hybrid strain using engineering restorer lines and engineering sterility lines by gene engineering technology.

关键词： maize inbred line Barstar gene particle bombardment transgenic plant molecular detection

Transformation of TrxS Gene into Barley by particle bombardment

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2005年第8期4卷 574-578页

作者： WEI Li YIN Jun KONG Wei-wei REN Jiang-ping LI Lei LIU Lei Henan Agricultural University National Engineering Research Center of Wheat Zhengzhou 450002 P.R.China

The production of malting barley in China can＇t meet the demand of beer industries because of poor quality and it becomes a bottleneck problem in beer manufacture industry. In this paper, TrxS gene cloned from Phalaris coerulescens was transferred into barley cultivar Yupi 1 （YP1） via biolistic bombardment. 1206 immature embryos were bombarded and seven transgenic plants carrying TrxS gene were confirmed by PCR and PCR-Southern blotting analysis. TrxS gene was expressed in transgenic plants by RT-PCR analysis. The activity of Trxh and α-amylase of transgenic line were higher than that of non-transgenic line, which is helpful to improve malting quality of barley.

关键词： Barley TrxS gene particle bombardment Transformation

Hereditary Behavior of bar Gene Cassette is Complex in Rice Mediated by particle bombardment

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2007年第9期34卷 824-835页

作者：赵艳钱前王慧中黄大年浙江工商大学食品生物与环境工程学院杭州310035 中国水稻研究所水稻生物学国家重点实验室杭州310006 杭州师范学院生命科学学院杭州310036

particle bombardment transformation using minimal gene cassette （containing the promoter, open reading frame and terminator） is the novel trend in plant genetic transformation, and its use helps to alleviate the undesirable effects of plasmid vector backbone sequences on transgenic plants. In the present article, studies related to the hereditary behavior of bar gene cassette in T1 to T3 generations of the transgenic rice （Oryza sativa L.） lines transformed by particle bombardment have been discussed. The selectable marker bar gene cassette that integrated with the rice genome had multiple copies and showed complex segregation behaviors including the presence of ‘false homozygotes’, with abnormal segregation ratios ranging from 35：1 to 144：1 （Basta-resistant： sensitive plants） in their progenies. In five out of ten original transgenic lines, bar gene can be stably transmitted as a dominant gene to self-pollinated T2 progeny. The homozygotes were obtained in three transgenic lines in T1 generation regardless of the multiple-copy integration patterns of bar gene. Southern blotting analysis showed that multiple copies of bar gene cassette were linked, which formed transgene arrays in the host rice genome. The authors also observed stable transmission of integration patterns of bar gene cassette, as obtained from Southern blotting analysis, in the regularly segregated transgenic rice lines and loss of gene in an irregularly segregated transgenic line. The segregation behavior varied among the transgenic progenies that exhibited similar Southern hybridization patterns of bar gene. On the basis of these results, the multiple-copy integration, gene lost, and gene expres- sion interaction were the major reasons for the complex segregation behaviors of bar gene cassette in transgenic rice plants.

关键词： bar gene cassette particle bombardment hereditary behavior rice

Conifer genetic engineering: particle bombardment and Agrobacterium-mediated gene transfer and its application in future forests

在线全文

学校读者我要写书评

暂无评论

Journal of Forestry Research 2001年第4期12卷 220-228,276页

作者：唐巍 NorthCarolinaStateUniversity RaleighNC27607USA

Many important advances in forest biotechnology have been made. The use of genetic transformation and the ap-plications of transgenic trees in modern forestry is now an important field. Two basic methodologies particle bombardment and Agrobacterium-mediated transformation have been used on conifers. However, routine procedures exist for only a limited number of conifers. As a result only a few species have been successfully transformed into stable transgenic plants. The use of a particle bombardment has been more successful and transgenic plants have been produced in Picea abies, Picea glauca, Picea mariana, and Pinus radiata, although the level of production of stable transgenic plants is lower than that of Agrobacte-rium. At present, breeding programs have been directed toward improving bole shape, growth rate, wood properties, and quality, as well as toward improving root and shoot performance, pest resistance, stress tolerance, herbicide resistance, and ability to resist stresses, which will drive forestry to enter a new era of productivity and quality. This article provides a brief overview of the current state of knowledge on genetic transformation in conifers.

关键词： Genetic engineering particle bombardment Agrobacterium Conifer

Study on the Obtaining of Transgenic Wheat with GNA Alien Gene by Biolistic particle

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2002年第1期1卷 25-29页

作者： XU Qiong-fang, LI Lian-cheng, CHEN Xiao, TIAN Fang, MA You-zhi, YE Xing-guo, ZHANG Zeng-yan, XU Hui-jun and XIN Zhi-yong( Key Laboratory of Agricultural Ministry for Crop Genetics and Breeding, Institute of Crop Breeding and Cultivation ,Chinese Academy of Agricultural Sciences, Beijing 100081 ) Key Laboratory of Agricultural Ministry for Crop Genetics and Breeding Institute of Crop Breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing 100081

The immature embryos of wheat plants, cv. Jing 411, 12 - 14 days after pollination, were cultured on SD2 medium for callus induction. After 10 days culture, 800 wheat calli were bombarded by biolistic particle coated with the DNA of plasmid pBI121-2 harboring both Galanthus nivalis agglutinin gene and bar gene. 67 green plants were finally regenerated from the bombardment calli on selection medium containing 4mg/L Basta. The results of bioassay by both inoculating wheat aphids onto the plants and applying Basta solution of 50 mg/L and 75 mg/L onto the wheat leaves in the field, and the molecular analysis, such as PCR and Southern blotting, indicated that 8 T2 plants contaning the target genes were obtained.

关键词： Galanthus nivalis agglutinin bar gene particle bombardment Transgenic wheat

Creation of Transgenic Bananas Expressing Human Lysozyme Gene for Panama Wilt Resistance

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2005年第8期47卷 971-977页

作者： Xin-Wu PEI Shi-Kai CHEN Rui-Ming WEN Shang YE Jia-Qin HUANG Yong-Qiang ZHANG Bing-Shan WANG Zhi-Xing WANG Shi-Rong JIA Biotechnology Institute Chinese Academy of Agricultural Sciences Beijing 100081 China Xinyi Institute of Agricultural Science and Technology Xinyi Guangdong 523530 China

Human lysozyme （HL） inhibits Fusarium oxysporum （FocR4） growth in vitro. To obtain transgenic bananas （Musa spp.） that are resistant to Panama wilt （F. oxysporum）, we introduced an HL gene that is driven by a constitutive cauliflower mosaic virus 35S promoter into the banana via Agrobacteriummediated transformation. PCR confirmed that 51 transgenic plants were obtained. The development of Panama wilt symptoms were examined after the plants had been grown in pots. The non-transgenic plants developed typical fusarium symptoms 60 d after FocR4 inoculation, whereas 24 of 51 transgenic plants remained healthy. The transgenic banana plants that showed resistance to FocR4 in the pots were then planted in a field that was heavily infected with FocR4 for further investigation. Eleven of 24 plants devel- oped symptoms before bud emergence; another 11 plants showed symptoms after bud emergence and the remaining two plants, H-67 and H-144, remained healthy and were able to fruit. Northern blotting analysis demonstrated that H-67 and H-144, bearing the strongest resistance to Panama wilt, had the highest level of HL expression and that the expression of HL was well correlated with the FocR4 resistance of transgenic plants. We conclude that Agrobacterium-mediated transformation, with the assistance of particle bombardment, is a powerful approach for banana transformation and that a transgenic HL gene can cause resistance of the crop to FocR4 in the field.

关键词： Agrobacterium-mediated transformation Fusarium oxysporum human lysozyme Panamawilt disease particle bombardment transgenic banana.

Transformation of Pea Lectin Gene and Parasponia Haemoglobin Gene into Rice and Their Expressions

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Botanica Sinica 2001年第3期43卷 267-274页

作者：张静娴王忆平沈世华王逸群高越峰单雪琴荆玉祥中国科学院植物研究所北京100093 北京大学生命科学学院北京100871

Lectin and leghemoglobin in legumes play the important roles, respectively, in recognition of host plants to their rhizobial bacteria, and lowering the oxygen partial pressure around bacteroids and protecting nitrogenase from oxygen in symbiotic nitrogen-fixing nodules. In order to extend the host range of the rhizobial bacteria and to make them fix nitrogen in non-legumes, pea lectin gene (pl) and Parasponia hemoglobin gene ( phl,) have been constructed into a plant expression vector (pCBHUL) and the vector pCBHUL was introduced into rice calli from immature young embryos by particle bombardment. After the calli were regenerated into plantlets on the resistant-selecting media containing hygromycin, they were identified by PCR and Southern blot hybridization. It was indicated that the pi and phb genes were integrated into nucleic genome of the transformed rice plants. GUS activity and the product of the pi gene were determined by GUS staining, Western blot and in situ hybridization at translational level. Eighteen out of 40 plants resistant to hygromycin were positively identified by PCR analysis with the rate of 45%. The pi gene was expressed in 3 out of 18 plants with 17% and 7.5% in 40 plants. The results may provide a clue for exploring whether Rhizobium leguminosarum by. viceae could extend its host range and make the transgenic rice plants have the possibility of being symbiotic, or associative to nitrogen fixation.

关键词： Parasponia hemoglobin gene pea lectin gene particle bombardment transformation gene expression transgenic rice

Novel Techniques for Gene Delivery into Plants and Its Applications for Disease Resistance in Crops

在线全文

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2016年第1期7卷 181-193页

作者： Ahmad Hama Ameen Rashid Djshwar Dhahir Lateef Field Crop Departments College of Agriculture University of Sulaimani Sulaimaniyah Iraq

From the early past to the present, biotechnologies have produced the ability to genetically transform a wide variety of plant species. The plant transformation technologies have changed the face of agriculture and plant biology. Plant genetic transformation is one of the key technologies for crop improvement in addition to emerging approach for producing recombinant proteins in plants. Both plastid genomes and plant nuclear can be genetically modified. Until now, essential functional differences between the prokaryotic-like genome of the plastid and the eukaryotic genome of the plant cell nucleus will have an impact on characteristics of transgenic organism. Thus, the main goals are to generate transgenic plants with the traits of interest as well as minimizing the amount of transgenic DNA in plants while maximizing stability of gene expression and trait performance. In this review, two broad groups of gene delivery methods will be discussed namely, (bilogical and physical methods) and subsequently there applications for improving disease resistance will be discussed.

关键词： T-DNA particle bombardment MSNs Silicon Carbide-Mediated Transformation Electroporation

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

草菇遗传转化体系建立的研究

草菇遗传转化体系建立的研究

作者：熊盛华南热带农业大学

学位级别：硕士

The straw mushroom, Volvariella volvacea, is one of the most important cultivated edible mushrooms, representing a valuable source both in economy and in nutrition. But the straw mushroom has comparably lower individual yield and less substrate utilization efficiency. It is also unable to stand freeze preservation. Thus breeding novel strains (highly resistant and high- yield strain) is one important problem in straw mushroom production and research.It is designed in this study to establish an efficient transformation system of straw mushroom with plasmid pCAMBIA1301 and recombinant plasmid pCTH823 by particle bombardment and Agrobacterium-mediated DNA entry. So that it can contribute to the genetic modification of straw mushroom varieties and can be helpful in the study of its functional genome.The main content of the study as follows:Optimization of parameters used in particle bombardment by visualization of the growth of putative transformants on selective medium.Establishment of transformation conditions applied in Agrobacteriwn-mediated DNA entry.Visualization of the expression of GUS gene in putative transformants using histochemical assay. The results show that GUS gene is integrated into the genome of straw mushroom.The results of Cropping trial and GUS enzyme activity assay indicated that straw mushroom transformants can develop into normal fruiting body and GUS gene can be expressed in this kind of fruiting body.The resistant strains of Straw mushroom obtained in the screening after bombardment were detected by Southern dot blotting. Positive signals were visualized after hybridization for 16 hours, which shows that GUS gene was integrated into the genome of straw mushroom from the level of gene.Straw mushroom transformed by /Igro&actermm-mediated DNA entry was assayed by PCR. 2 strains gave positive signals, which proves that transgenic straw mushroom was obtained.

关键词： Straw mushioom(Volvariella volvacea) genetic transformation GUS gene particle bombardment Agrobacterium-mediated DNA entry THP gene