Transfer and Detection of barstar Gene to Maize Inbred Line 18-599 (White) by Particle Bombardment
Transfer and Detection of barstar Gene to Maize Inbred Line 18-599 (White) by Particle Bombardment作者机构：Shandong Agricultural University/State Key Laboratory of Crop Biology Taian 271018 P.R.China Sichuan Agricultural University Yaan 625014 P.R.China
出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)
年 卷 期：2007年第6卷第6期
页 面：652-656页
学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学]
基 金：Lu TA 2004 113 2005BS06010 2006CB 1017 00
主 题：maize inbred line Barstar gene particle bombardment transgenic plant molecular detection
摘 要：In China, the purity of maize hybrid strain is discomforting to the development of seed industrialization. Finding a new method for reproduction of maize hybrid strain is necessary. In this study, using particle bombardment, barstar gene was transferred into maize inbred line 18-599 （White）, which is an antiviral and high quality maize inbred line. By molecular detection of the anther of transgenic maize, two plants transferred with barstar gene were gained in this study, which are two restorer lines. The two plants showed normal male spike, and lively microspores. But the capacity of the two restorer lines should be studied in the future. The aim of this study is to find a new method of reproduction of maize hybrid strain using engineering restorer lines and engineering sterility lines by gene engineering technology.
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