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Cloning and analysis of cross-intron genomic gene encoding ACO in paeonia suffruticosa Andr.

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Forestry Studies in China 2012年第4期14卷 291-298页

作者： FAN Bing-you GAO Shui-pingCollege of Forestry Henan University of Science and Technology HOU Xiao-gai SHI Guo-an KONG Xiang-sheng College of Agriculture Henan University of Science and Technology

The last step of ethylene biosynthesis in higher plants is to convert 1-aminocyclopropane-1-carboxylic acid （ACC） to ethylene by ACC oxidase （ACO）. In our investigation, a cross-introns genomic DNA gene encoding ACC oxidase, PsgACO, was isolated from paeonia suffruticosa. It revealed that PsgACO （FJ855434） has 1281 bases, containing four exons and three introns, en- coding 312 amino acids. The four exons stretched from 1 to 105, 217 to 434, 592 to 925 and 1000 to 1281 bases. A splicing junction sequence of all the exon-introns conformed to the GT-AG rule in the cross-intron genomic DNA sequence of the ACO gene. PsgACO showed high homology to many characterized ACC oxidases both at the nucleic acid and amino acid levels. As well, twelve amino acid residues were conserved among many ACOs from other species. A phylogenetic tree analysis indicated that the amino acid of ACOs is quite conserved among the different eudicots. The phylogenetic tree showed that both the tree peony and herbaceous peony are quite isolated taxa. Bioinformatic analysis showed that the molecular weight of ACO is 35.29 kD, with a theoretical pI of 5.25. It is a non-secrete, stable hydrophilic protein, located in the cytoplasm.

关键词： paeonia suffruticosa A CO cloning sequence analysis

Transcriptome-based identification and expression analysis of the glutathione S-transferase(GST)family in tree peony reveals a likely role in anthocyanin transport

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Horticultural Plant Journal 2022年第6期8卷 787-802页

作者： Lulu Han Hongzhu Zou Lin Zhou Yan Wang Research Institute of Forestry Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland AdministrationChinese Academy of ForestryBeijing 100091China

For red-flowered cultivars of tree peony(paeonia suffruticosa),anthocyanin content is a critical factor determining the different petal pigmentations.Glutathione S-transferases(GSTs)are ubiquitous and multifunctional conjugating proteins that may be responsible for the transport of anthocyanin pigments from the cytoplasm to vacuole.The underlying function of the GST family in tree peony,however,remains unclear.In this study,we systematically isolated and identified a total of 54 putative full-length Ps GST genes through a combination of bioinformatics approaches from transcriptome databases.Intraspecific phylogenetic analyses revealed extensive differentiation in their coding sequences and divided them into 10 of the 14 known classes of plant GSTs.The phylogenetic relationships,evolutionary characteristics,protein domain,and motif organization were clearly conserved among the different phylogenetic subclasses.The results of the RNA-seq and quantitative real-time polymerase chain reaction experiments exhibited extensive variation in gene expression profiles among different developmental stages and varieties.Furthermore,the phylogenetic relationships,expression profiles,protein interactions,weighted gene co-expression network analysis,and correlation analysis results suggested that PsGSTF3(Unigene 0064200)is a candidate participant in anthocyanin transport and the promotion of pigment accumulation,exhibiting a strong positive correlation with anthocyanin content among different tissues(r=0.908**)and an increasing rate of anthocyanin content during the flower developmental process(r=0.961*).These results furthered our understanding of the transport and accumulation functions of the GST family as well as the enhancement of tree peony breeding through molecular biology techniques.

关键词： GST paeonia suffruticosa Functional prediction Expression analysis Anthocyanin transportation

内源抗氧化酶在牡丹开放和衰老进程中作用的研究

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内源抗氧化酶在牡丹开放和衰老进程中作用的研究

作者：周昕蕾北京林业大学

学位级别：硕士

本研究以牡丹‘洛阳红’为试验材料,通过对牡丹自然开放进程中形态特征变化的观测,明确了牡丹盆花采后观赏期划分为4个阶段：蕾期、观赏初期、最佳观赏期、观赏末期。同时,对不同栽培条件下牡丹采后开放和衰老进程中内源抗氧化酶系统的... 详细信息

本研究以牡丹‘洛阳红’为试验材料,通过对牡丹自然开放进程中形态特征变化的观测,明确了牡丹盆花采后观赏期划分为4个阶段：蕾期、观赏初期、最佳观赏期、观赏末期。同时,对不同栽培条件下牡丹采后开放和衰老进程中内源抗氧化酶系统的作用进行了研究。试验结果如下： 1.自然条件下牡丹花朵采后开放进程中乙烯生产量表现出前期上升,出现峰值后下降,衰老过程中又出现回升的趋势。SOD、CAT活性与乙烯生成量的变化趋势一致。POD活性不受乙烯生成量的影响,在开放初期活性较低,而在衰老过程中,其活性剧烈增强。 2.牡丹盆花中SOD活性与自然条件下表现一致,随着乙烯生成量的变化而变化。CAT活性在盛花期出现峰值。POD活性显现出先上升后下降末期再上升的变化趋势,说明POD作为有效抵御有氧胁迫的内在防御工具,在牡丹花朵衰老过程中发挥了积极的保护效应。 3.在切花采后开放进程中,1-MCP处理抑制了内源乙烯的生成量,SOD、POD活性降低,CAT活性增强。外源乙烯处理后同样抑制了内源乙烯的生产量,SOD、CAT活性随之降低,POD活性与乙烯生产量没有明显联系。在衰老过程中,1-MCP处理后内源乙烯生成量减少,此时CAT、POD活性却出现增强的趋势。说明离体条件下乙烯代谢影响到抗氧化酶的活性,改变了采后瓶插寿命,最终影响到采后品质。其中SOD、CAT活性在开放进程中与乙烯代谢关系紧密,而POD在衰老过程中发挥着重要的作用。

关键词： paeonia suffruticosa 乙烯 SOD POD CAT

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牡丹品种分类及观赏性状的主成分分析

林业科技通讯 2020年第5期 32-35页

作者：丁丽萍马力冉永军张掖市林业科学研究院甘肃张掖734000 河西学院甘肃张掖734000

对引种栽培的32个牡丹(paeonia suffruticosa)品种进行分类鉴定,采用主成分分析法对6个主要观赏性状进行分析,并对不同品种的观赏性进行综合评价。结果表明,通过主成分分析可将原6个性状综合为4个主成分,其累计贡献率达81.5%。以4个主... 详细信息

对引种栽培的32个牡丹(paeonia suffruticosa)品种进行分类鉴定,采用主成分分析法对6个主要观赏性状进行分析,并对不同品种的观赏性进行综合评价。结果表明,通过主成分分析可将原6个性状综合为4个主成分,其累计贡献率达81.5%。以4个主成分的贡献率为权重建立观赏性评价模型,对32个品种进行综合评分,从而选择出观赏性状优良的牡丹品种,为牡丹选优推广提供理论依据。

关键词：牡丹 paeonia suffruticosa 品种分类主成分分析观赏性能评价

Paeonol inhibits proliferation of vascular smooth muscle cells stimulated with high glucose concentrations by inhibiting the Ras-Raf-ERK1/2 signaling pathway

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Paeonol inhibits proliferation of vascular smooth muscle cel...