Cloning and analysis of cross-intron genomic gene encoding ACO in Paeonia suffruticosa Andr.

作     者：FAN Bing-you GAO Shui-pingCollege of Forestry Henan University of Science and Technology HOU Xiao-gai SHI Guo-an KONG Xiang-sheng 

作者机构：College of AgricultureHenan University of Science and Technology 

出 版 物：《Forestry Studies in China》 (中国林学（英文版）)

年 卷 期：2012年第14卷第4期

页      面：291-298页

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 090706[农学-园林植物与观赏园艺] 0907[农学-林学] 0829[工学-林业工程] 09[农学] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China (No. 30740013) the Foundation for Young University Key Teachers of the Educational Commission of Henan Province, China(No. 2010GGJS-075) 

主　　题：Paeonia suffruticosa A CO cloning sequence analysis 

摘      要：The last step of ethylene biosynthesis in higher plants is to convert 1-aminocyclopropane-1-carboxylic acid （ACC） to ethylene by ACC oxidase （ACO）. In our investigation, a cross-introns genomic DNA gene encoding ACC oxidase, PsgACO, was isolated from Paeonia suffruticosa. It revealed that PsgACO （FJ855434） has 1281 bases, containing four exons and three introns, en- coding 312 amino acids. The four exons stretched from 1 to 105, 217 to 434, 592 to 925 and 1000 to 1281 bases. A splicing junction sequence of all the exon-introns conformed to the GT-AG rule in the cross-intron genomic DNA sequence of the ACO gene. PsgACO showed high homology to many characterized ACC oxidases both at the nucleic acid and amino acid levels. As well, twelve amino acid residues were conserved among many ACOs from other species. A phylogenetic tree analysis indicated that the amino acid of ACOs is quite conserved among the different eudicots. The phylogenetic tree showed that both the tree peony and herbaceous peony are quite isolated taxa. Bioinformatic analysis showed that the molecular weight of ACO is 35.29 kD, with a theoretical pI of 5.25. It is a non-secrete, stable hydrophilic protein, located in the cytoplasm.