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The MADS transcription factor CmANR1 positively modulates root system development by directly regulating Cmpin2 in chrysanthemum

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Horticulture Research 2018年第1期5卷 202-217页

作者： Cui-Hui Sun Jian-Qiang Yu Xi Duan Jia-Hui Wang Quan-Yan Zhang Kai-Di Gu Da-Gang Hu Cheng-Shu Zheng National Key Laboratory of Crop Biology College of Horticulture Science and EngineeringShandong Agricultural UniversityTai-AnShandong271018China Shandong Agricultural and Engineering University Ji-NanShandongChina

Plant root systems are essential for many physiological processes,including water and nutrient ***-box transcription factor(TF)genes have been characterized as the important regulators of root development in plants;however,the underlying mechanism is largely unknown,including ***,it was found that the overexpression of CmANR1,a chrysanthemum MADS-box TF gene,promoted both adventitious root(AR)and lateral root(LR)development in *** transcriptome sequencing analysis revealed a series of differentially expressed unigenes(DEGs)in the roots of CmANR1-transgenic chrysanthemum plants compared to wild-type *** annotation of these DEGs by alignment with Gene Ontology(GO)terms and biochemical pathway Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis indicated that CmANR1 TF exhibited“DNA binding”and“catalytic”activity,as well as participated in“phytohormone signal transduction”.Both chromatin immunoprecipitation–polymerase chain reaction(ChIP-PCR)and gel electrophoresis mobility shift assays(EMSA)indicated the direct binding of Cmpin2 to the recognition site CArG-box motif by ***,a firefly luciferase imaging assay demonstrated the transcriptional activation of Cmpin2 by CmANR1 in ***,our results provide novel insights into the mechanisms of MADS-box TF CmANR1 modulation of both AR and LR development,which occurs by directly regulating auxin transport gene Cmpin2 in chrysanthemum.

关键词： pin2 Whole alignment

Transmembrane kinase 1-mediated auxin signal regulates membrane-associated clathrin in Arabidopsis roots

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Journal of Integrative Plant Biology 2023年第1期65卷 82-99页

作者： Yutong Wang Xu Yan Mei Xu Weiyang Qi Chunjie Shi Xiaohong Li Jiaqi Ma Dan Tian Jianxin Shou Haijun Wu Jianwei Pan Bo Li Chao Wang Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations School of Life Sciences Lanzhou UniversityLanzhou 730000 China College of Life Sciences Shaoxing University Shaoxing 312000 China

Clathrin-mediated endocytosis(CME)is the major endocytic pathway in eukaryotic cells that directly regulates abundance of plasma membrane *** triskelia are composed of clathrin heavy chains(CHCs)and light chains(CLCs),and the phytohormone auxin differentially regulates membraneassociated CLCs and CHCs,modulating the endocytosis and therefore the distribution of auxin efflux transporter pin-FORMED2(pin2).However,the molecular mechanisms by which auxin regulates clathrin are still poorly *** kinase(TMKs)family proteins are considered to contribute to auxin signaling and plant development;it remains unclear whether they are involved in pin transport by *** assessed TMKs involvement in the regulation of clathrin by auxin,using genetic,pharmacological,and cytological approaches including live-cell imaging and *** tmk1 mutant seedlings,auxin failed to rapidly regulate abundance of both CHC and CLC and to inhibit pin2 endocytosis,leading to an impaired asymmetric distribution of pin2 and therefore ***,TMK3 and TMK4 were shown not to be involved in regulation of clathrin by *** summary,TMK1 is essential for auxin-regulated clathrin recruitment and ***1therefore plays a critical role in the establishment of an asymmetric distribution of pin2 and an auxin gradient during root gravitropism.

关键词： Arabidopsis auxin clathrin endocytosis pin2 transmembrane kinase1(TMK1)

The unconventional prefoldin RPB5 interactor mediates the gravitropic response by modulating cytoskeleton organization and auxin transport in Arabidopsis

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Journal of Integrative Plant Biology 2022年第10期64卷 1916-1934页

作者： Yi Yang Fang Liu Le Liu Mingyue Zhu Jinfeng Yuan Yan‐Xia Mai Jun‐Jie Zou Jie Le Yonghong Wang Klaus Palme Xugang Li Yong Wang Long Wang State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityDaizong Street 61Tai'an 271018China Shandong Provincial Key Laboratory of Biophysics Institute of BiophysicsDezhou UniversityDezhou 253023China Sino‐German Joint Research Center on Agricultural Biology College of Life SciencesShandong Agricultural UniversityDaizong Street 61Tai'an 271018China Faculty of Biology Institute of Biology II/Molecular Plant PhysiologyAlbert‐Ludwigs‐University of FreiburgSchänzlestrasse 1Freiburg D‐79104Germany National Key Laboratory of Plant Molecular Genetics(NKLPMG) CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and Ecology(SIPPE)Chinese Academy of Sciences(CAS)Shanghai 200032China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Plant Molecular Physiology CAS Center for Excellence in Molecular Plant SciencesInstitute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China

Gravity-induced root curvature involves the asymmetric distribution of the phytohormone *** response depends on the concerted activities of the auxin transporters such as pin-FORMED(pin)proteins for auxin efflux and AUXIN RESISTANT 1(AUX1)for auxin ***,how the auxin gradient is established remains *** we identified a new mutant with a short root,strong auxin distribution in the lateral root cap and an impaired gravitropic *** causal gene encoded an Arabidopsis homolog of the human unconventional prefoldin RPB5 interactor(URI).At URI interacted with prefoldin 2(PFD2)and PFD6,twoβ-type PFD members that modulate actin and tubulin patterning in *** auxin reporter DR5_(rev):GFP showed that asymmetric auxin redistribution after gravistimulation is disordered in aturi-1 root *** with the endomembrane protein trafficking inhibitor brefeldin A indicated that recycling of the auxin transporter pin2 is disrupted in aturi-1 roots as well as in pfd *** propose that At URI cooperates with PFDs to recycle pin2and modulate auxin distribution.

关键词： Arabidopsis auxin cytoskeleton pin2 prefoldin(PFD) unconventional prefoldin RPB5 interactor(URI)

Titanium Dioxide Nanoparticles Promote Root Growth by Interfering with Auxin Pathways in Arabidopsis thaliana

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Phyton-International Journal of Experimental Botany 2020年第4期89卷 883-891页

作者： Jiali Wei Ying Zou ping Li Xiaojun Yuan Shanghai Key Laboratory of Bio-Energy Crops Research Center for Natural ProductsPlant Science CenterSchool of Life SciencesShanghai UniversityShanghai200444China

TiO_(2) nanoparticles(nano-TiO_(2))are widely used in the world,and a considerable amount of nano-TiO_(2) is released into the environment,with toxic effects on *** the various species of higher plants,growth,including seed germination,root elongation,and biomass accumulation,is affected by nano-TiO_(2).However,the underlying molecular mechanisms remain to be *** this study,we observed that nano-TiO_(2) promoted root elongation in a dose-dependent ***,we found that nano-TiO_(2) elevated auxin accumulation in the root tips of the auxin marker lines DII-VENUS and DR5::GUS,and,correspondingly,quantitative real-time PCR analysis revealed that nano-TiO_(2) increased the expression levels of auxin biosynthesis-and transport-related *** fluorescence observation using transgenic pin2-GFP indicated that nano-TiO_(2) promoted root growth by inducing pin2 ***,we propose that nano-TiO_(2) promote root growth in Arabidopsis thaliana by altering the expression levels of auxin biosynthesis-and transport-related genes.

关键词： Nanoparticles nano-TiO_(2) root length auxin pin2 auxin-related genes

pin FORMED 2 Modulates the Transport of Arsenite in Arabidopsis thaliana

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Plant Communications 2020年第3期1卷 42-56页

作者： Mohammad Arif Ashraf Kana Umetsu Olena Ponomarenko Michiko Saito Mohammad Aslam Olga Antipova Natalia Dolgova Cheyenne D.Kiani Susan Nehzati Keitaro Tanoi Katsuyuki Minegishi Kotaro Nagatsu Takehiro Kamiya Toru Fujiwara Christian Luschnig Karen Tanino Ingrid Pickering Graham N.George Abidur Rahman United Graduate School of Agricultural Sciences Iwate UniversityMoriokaIwateJapan Department of Plant Bio Sciences Faculty of AgricultureIwate UniversityMoriokaIwateJapan Molecular and Environmental Science Research Group Department of Geological SciencesUniversity of SaskatchewanSaskatoonSKCanada Argonne National Lab Advanced Photon SourceXSD-MICLemontILUSA Isotope Facility for Agricultural Education and Research Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuTokyoJapan Department of Radiopharmaceuticals Development National Institute of Radiological SciencesNational Institutes for Quantum and Radiological Science and TechnologyInageChibaJapan Department of Applied Biological Chemistry Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuTokyoJapan Department of Applied Genetics and Cell Biology University of Natural Resources and Life SciencesVienna(BOKU)Muthgasse 181180 WienAustria Department of Plant Sciences College of Agriculture and BioresourcesUniversity of SaskatchewanSaskatoonSKCanada Agri-Innovation Center Iwate UniversityMoriokaIwateJapan

Arsenic contamination is a major environmental issue,as it may lead to serious health *** reduced trivalent formof inorganic arsenic,arsenite,is in generalmore toxic to plants comparedwith the fully oxidized pentavalent *** arsenite inplants hasbeenshown tobemediatedthrough a large subfamily of plant aquaglyceroporins,nodulin 26-like intrinsic proteins(NIPs).However,the efflux mechanisms,as well as themechanismof arsenite-induced root growth inhibition,remain poorly *** physiology,synchrotron imaging,and root transport assay approaches,we show that the cellular transport of trivalent arsenicals inArabidopsis thalianais stronglymodulatedbypinFORMED2(pin2)auxin efflux *** transport assay using radioactive arsenite,X-ray fluorescence imaging(XFI)coupled with X-ray absorption spectroscopy(XAS),and inductively coupled plasma mass spectrometry analysis revealed that pin2 plants accumulate higher concentrations of arsenite in roots comparedwith the *** the cellular level,arsenite specifically targets intracellular sorting of pin2 and thereby alters the cellular auxin ***,loss of pin2 function results in arsenite hypersensitivity in *** coupled with XAS further revealed that loss of pin2 function results in specific accumulation of arsenical species,but not the other metals such as iron,zinc,or calcium in the root ***,these results suggest that pin2 likely functions as an arsenite efflux transporter for the distribution of arsenical species in planta.

关键词： auxin arsenite pin2 trafficking transport

An Arabidopsis PAK Family Protein Kinase Participates in Cell Size Determination and Gravitropism Response

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An Arabidopsis PAK Family Protein Kinase Participates in Cel...

Cytoplasmic auxin signaling

植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Xiulian Yu,Jingfeng Zhou,and Qingqiu Gong~* Department of Plant Biology and Ecology,College of Life Sciences,Nankai University,Tianjin 300071,China

Yeast and mammalian p21-activated kinase(PAK) family members regulate fundamental cellular processes including the cell cycle,apoptosis,vacuole inheritance, cell size determination,and sterol biosynthesis[1-2].We iden... 详细信息

关键词： PAK cell size gravitropism pin2 sterol BR

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Cytoplasmic auxin signaling

An Arabidopsis PAK Family Protein Kinase Participates in Cell Size Determination and Gravitropism Response

从植物科学到农业发展——2012全国植物生物学大会

作者： Zhenbiao Yang Department of Botany and Plant Sciences and Center for Plant Cell Biology,University of California at Riverside,CA 92521,USA Shanghai Institute of Plant Physiology and Ecology,Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,Shanghai,China.

Auxin regulates the formation of various developmental and morphogenetic patterns in plants,but the molecular and cellular mechanisms for the auxin actions are not well *** investigate these mechanisms using Arabidopsis leaf epidermal pavement cells(PCs) as a model system,which form the jigsaw puzzle cell pattern with interdigitated lobes and *** have shown that pavement cell interdigitation is controlled by localized ROP GTPase signaling,which impinges on the organization of cortical actin microfilaments and microtubules to generate interdigitating lobes and *** recent studies indicate that auxin is a signal that activates the generation of multi-polarity to form lobes in pavement cells and coordinates lobe formation with indentation formation by activating ROP GTPase signaling through an Auxin-Binding Protein 1(ABP1) dependent auxin perception *** cytoplasmic auxin signaling system,which is distinct from the well-established TIR1-dependent nuclear auxin signaling system that regulates gene transcription,modulates local coordination of lobe and indentation formation within a PC and between adjacent PCs through its modulation of pin protein *** ROP-based cytoplasmic auxin signaling also regulates the subcellular distribution of pins in root cells,supporting a widespread role for this new auxin signaling system in plants 4. Recent findings of additional components in this new auxin signaling system will be discussed.

关键词： AUXIN SIGNALING ABP1 pin1 pin2

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拟南芥EXO70B家族蛋白调控生长素极性运输的初步研究

2010全国植物生物学研讨会

作者： Xiulian Yu Jingfeng Zhou Qingqiu Gong Department of Plant Biology and Ecology College of Life Sciences Nankai University

Yeast and mammalian p21-activated kinase(PAK)family members regulate fundamental cellular processes including the cell cycle,apoptosis,vacuole inheritance,cell size determination,and sterol biosynthesis[1-2].We identi... 详细信息

Yeast and mammalian p21-activated kinase(PAK)family members regulate fundamental cellular processes including the cell cycle,apoptosis,vacuole inheritance,cell size determination,and sterol biosynthesis[1-2].We identified a PAK in Arabidopsis and isolated a T-DNA insertion line,in which the protein kinase domain

关键词： PAK cell size gravitropism pin2 sterol BR

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拟南芥EXO70B家族蛋白调控生长素极性运输的初步研究

作者：朱娜山东农业大学

学位级别：硕士

植物激素生长素几乎调控着植物生长和发育的所有方面。极性生长素的运输是通过生长素外运载体pin介导的,pin蛋白在质膜上极性定位。在整个发育过程中生长素能够快速的调控基因转录,主要是通过快速调控Aux/IAA蛋白的水平。胞吐是植物中... 详细信息

植物激素生长素几乎调控着植物生长和发育的所有方面。极性生长素的运输是通过生长素外运载体pin介导的,pin蛋白在质膜上极性定位。在整个发育过程中生长素能够快速的调控基因转录,主要是通过快速调控Aux/IAA蛋白的水平。胞吐是植物中一个非常重要的过程,胞吐被胞吐复合体所介导,胞吐复合体由8个亚基组成,其中包括EXO70。在拟南芥中EXO70包含有23个成员。我们获得了一个T-DNA插入的功能缺失型突变体exo70B1-3。通过对突变体的转录水平的检测,突变体中的转录水平明显降低。exo70B1-3突变体在拟南芥植株中有着明显的表型,与野生型相比,在幼苗期侧根数目较多。在侧器官中表达,响应生长素的基因LOB在突变体根中的转录水平明显升高。突变体的莲座叶较小且发黄,向地性加快。在不同浓度的NAA处理实验中,突变体表现出主根抑制率较大,LOB的转录水平发生紊乱。突变体根中的DR5::GFP明显增强,这暗示着突变体内生长素水平发生紊乱。pin2是生长素极性外运蛋白,极性定位在质膜的顶端,突变体内的pin2循环迟移。可能是突变体exo70B1-3可以影响pin蛋白的极性定位,从而进一步影响生长素的转录调控,影响植物的生长发育。通过酵母双杂实验发现EXO70B1,EXO70B2与Aux/IAA互作,其中包括IAA2,IAA5,IAA17,IAA18等Aux/IAA蛋白。通过蛋白截短进一步发现EXO70B1,EXO70B2可与Aux/IAA的功能域IV相互作用,且Aux/IAA是与EXO70B1的N端相互作用的。通过GUS染色发现EXO70B1,EXO70B2和IAA5组织特异性相似,进一步增强它们之间互作的可能性。通过LUC荧光进一步验证蛋白之间的互作关系。

关键词：生长素 pin2 Aux/IAA 胞吐侧根