The highly organized extracellular matrix(ECM)of musculoskeletal tissues,encompassing tendons,ligaments and muscles,is structurally anisotropic,hierarchical and *** features collectively contribute to their unique ***...
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The highly organized extracellular matrix(ECM)of musculoskeletal tissues,encompassing tendons,ligaments and muscles,is structurally anisotropic,hierarchical and *** features collectively contribute to their unique *** studies have investigated the effect of tissue-engineered scaffold anisotropy on cell morphology and organization for musculoskeletal tissue repair and regeneration,but the hierarchical arrangement of ECM and compartmentalization are not typically ***,we present a method for multi-compartmental scaffold design that allows for physical mimicry of the spatial architecture of musculoskeletal tissue in regenerative *** design is based on an ECM-inspired macromolecule ***(pcl)scaffolds were fabricated with aligned fibers by electrospinning and mechanical stretching,and then surface-functionalized with the cell-supporting ECM protein molecule,tropoelastin(TE).TE was attached using two alternative methods that allowed for either physisorption or covalent attachment,where the latter was achieved by plasma ion immersion implantation(PIII).Aligned fibers stimulated cell elongation and improved cell alignment,in contrast to randomly oriented *** coatings bound by physisorption or covalently following 200 s PIII treatment promoted fibroblast *** represents the first cytocompatibility assessment of novel PIII-treated TE-coated pcl *** demonstrate their versatility,these 2D anisotropic pcl scaffolds were assembled into 3D hierarchical constructs with an internally compartmentalized structure to mimic the structure of musculoskeletal tissue.
A core-shell hybrid nanostructure,a hard core of single-crystalline lanthanum hydroxide nanowires and a soft shell of brushes of poly (ε-caprolactone) (pcl), has been successfully prepared by ring-opening polymerizat...
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A core-shell hybrid nanostructure,a hard core of single-crystalline lanthanum hydroxide nanowires and a soft shell of brushes of poly (ε-caprolactone) (pcl), has been successfully prepared by ring-opening polymerization (ROP) on the surface of nanowires. Such special structures were proved by TEM images. Meanwhile, the thickness of polymer layers was measured. The chemical component and the grafted pcl quantity of the sample were characterized by FTIR and TGA, which provides a simple and universal method to functionalize non-carbon nanowires. The modified lanthanum hydroxide nanowires can be well-dispersed in good solvents for pcl.
The use of bioactive glass(BG)particles as a filler for the development of composite electrospun fibers has already been widely reported and *** novelty of the present research work is represented by the use of benign...
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The use of bioactive glass(BG)particles as a filler for the development of composite electrospun fibers has already been widely reported and *** novelty of the present research work is represented by the use of benign solvents(like acetic acid and formic acid)for electrospinning of composite fibers containing BG particles,by using a blend of pcl and *** this work,different BG particle sizes were investigated,namely nanosized and micron-sized.A preliminary investigation about the possible alteration of BG particles in the electrospinning solvents was performed using SEM *** obtained composite fibers were investigated in terms of morphological,chemical and mechanical *** in vitro mineralization assay in simulated body fluid(SBF)was performed to investigate the capability of the composite electrospun fibers to induce the formation of hydroxycarbonate apatite(HCA).
Poly(e-caprolactone)(pcl)is widely adopted as an ingredient for tissue engineering *** improve its cell affinity,in this study,we developed a new method to introduce bioactive RGD peptides onto the surface of pcl via ...
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Poly(e-caprolactone)(pcl)is widely adopted as an ingredient for tissue engineering *** improve its cell affinity,in this study,we developed a new method to introduce bioactive RGD peptides onto the surface of pcl via condensation reaction between 2-cyanobenzothiazole(CBT)and *** pcl fibrous membranes were prepared by electrospinning,and RGD functionalization was characterized by fluorescence microscopy,scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS)and water contact angle(WCA).As expected,our results demonstrated the successful RGD immobilization on the surface of *** modification improved the hydrophilicity of pcl,changing their WCA from 112.20°to38.35°.Cell adhesion,spreading and proliferation of 3T3fibroblasts were also *** therefore believe that the methods reported in this study was facile and effective for functional modification of the hydrophobic pcl *** moderate reaction conditions are also suitable for covalent immobilization of bioactive molecules onto pcl.
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