Development of tropoelastin-functionalized anisotropic PCL scaffolds for musculoskeletal tissue engineering
作者机构：Charles Perkins CentreThe University of SydneySydneyNSW 2006Australia School of Life and Environmental SciencesThe University of SydneySydneyNSW 2006Australia Applied and Plasma Physics LaboratorySchool of PhysicsThe University of SydneySydneyNSW 2006Australia School of Biomedical EngineeringThe University of SydneySydneyNSW 2006Australia Sydney Nano InstituteThe University of SydneySydneyNSW 2006Australia
出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)
年 卷 期：2023年第10卷第1期
页 面：64-72页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 10[医学]
基 金：supported by an Australian Commonwealth Government Research Training Program Tuition Fee Offset and Stipend Scholarship.A.S.W.acknowledges funding from the National Health and Medical Research Council(APP1195827) M.M.M.B.acknowledges funding from the Australian Research Council(FL190100216)
主 题：tropoelastin PCL musculoskeletal tissue engineering plasma immersion ion implantation electrospinning anisotropy hierarchical
摘 要：The highly organized extracellular matrix(ECM)of musculoskeletal tissues,encompassing tendons,ligaments and muscles,is structurally anisotropic,hierarchical and *** features collectively contribute to their unique *** studies have investigated the effect of tissue-engineered scaffold anisotropy on cell morphology and organization for musculoskeletal tissue repair and regeneration,but the hierarchical arrangement of ECM and compartmentalization are not typically ***,we present a method for multi-compartmental scaffold design that allows for physical mimicry of the spatial architecture of musculoskeletal tissue in regenerative *** design is based on an ECM-inspired macromolecule ***(PCL)scaffolds were fabricated with aligned fibers by electrospinning and mechanical stretching,and then surface-functionalized with the cell-supporting ECM protein molecule,tropoelastin(TE).TE was attached using two alternative methods that allowed for either physisorption or covalent attachment,where the latter was achieved by plasma ion immersion implantation(PIII).Aligned fibers stimulated cell elongation and improved cell alignment,in contrast to randomly oriented *** coatings bound by physisorption or covalently following 200 s PIII treatment promoted fibroblast *** represents the first cytocompatibility assessment of novel PIII-treated TE-coated PCL *** demonstrate their versatility,these 2D anisotropic PCL scaffolds were assembled into 3D hierarchical constructs with an internally compartmentalized structure to mimic the structure of musculoskeletal tissue.
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