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Genetic Analysis of the p1 region of Human Enterovirus 71 Strains and Expression of the 55 F StrainVp1 protein

Virologica Sinica 2012年第1期27卷 10-18页

作者： Jian-qiang Li Jun-jie Yang Xiu-juan Fan Zhen-peng Sun Yan Sun Huan Li Zi-xin Meng Wei Li Lanzou Institute of Biological products Lanzhou 730070China

Enterovirus 71 （EV71） is a member of the Entero-virus genus of the picomaviridae family and is the major cause of Hand, foot, and mouth disease （HFMD） in children. Different strains from Gansu were cloned and the p1 protein was sequenced and analysed. Results indicate that there are three kinds of EV71 infections prevalent in Gansu. The Vp 1 protein from one of these strains, 55F, was expressed. The recombinant protein was expressed with high level and reacted specifically with the EV71 patient antibody, the recombinant protein was also applied to raise antiserum in rabbits and after the fourth injection a high titer of antiserum was detected by ELISA assay. These data are useful for further clarification of prevalent EV71 strains in the north of China at the molecular level and provide a basis for EV71 diagnosis.

关键词： EV71 Genetic analysis p1 region Expression Vp1 protein

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Genetic analysis of the p1 region of human enterovirus 71 strains isolated in Gansu during 2008—2009 and expression of the 55 F strain Vp1 protein

Genetic analysis of the P1 region of human enterovirus 71 st...

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2011中国生物制品年会暨第十一次全国生物制品学术研讨会

作者： LI Jian-qiang1,LI Wei1,YANG Jun-jie1,FAN Xiu-juan1,ZHAO Xing-wen1,WANG Yu-lin2 (1.Lanzhou Institute of Biological products,Lanzhou 730046,China 2.National Vaccine and Serum Institute,Beijing 100024,China)

Enterovirus 71(EV71) is a member of the Entero-virus genus of the picornaviridae family and is the major cause of hand,foot,and mouth disease(HFMD) in *** sequence of different strains from Gansu were cloned and anlysised,it indicated there were three kids of EV71 infections prevalented in Gansu.55F,one of the strains,Vp1 protein was *** recombinant protein expressed with high level and reacted specifically with the EV71 patient antibody, the recombinant protein was also applied to raise antiserum in rabbits and the high titer of antiserum was detected after the fouth injection by the ELISA *** data are useful further study molecular prevalent in the north of China and provided basis for EV71 diagnosis.

关键词： EV71 genetic analysis p1 region expression Vp1 protein

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