Genetic Analysis of the P1 Region of Human Enterovirus 71 Strains and Expression of the 55 F StrainVP1 Protein

作     者：Jian-qiang Li Jun-jie Yang Xiu-juan Fan Zhen-peng Sun Yan Sun Huan Li Zi-xin Meng Wei Li 

作者机构：Lanzou Institute of Biological ProductsLanzhou 730070China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2012年第27卷第1期

页      面：10-18页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Major S&T Special Projects of Gansu(092GKDA010) 

主　　题：EV71 Genetic analysis P1 region Expression VP1 protein 

摘      要：Enterovirus 71 （EV71） is a member of the Entero-virus genus of the Picomaviridae family and is the major cause of Hand, foot, and mouth disease （HFMD） in children. Different strains from Gansu were cloned and the P1 protein was sequenced and analysed. Results indicate that there are three kinds of EV71 infections prevalent in Gansu. The VP 1 protein from one of these strains, 55F, was expressed. The recombinant protein was expressed with high level and reacted specifically with the EV71 patient antibody, the recombinant protein was also applied to raise antiserum in rabbits and after the fourth injection a high titer of antiserum was detected by ELISA assay. These data are useful for further clarification of prevalent EV71 strains in the north of China at the molecular level and provide a basis for EV71 diagnosis.