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Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli

Asian Pacific Journal of Tropical Medicine 2022年第2期15卷 71-78页

作者： Guilherme S.Machado Rosane L.Lopes Simone S.C.Oliveira Simone P.C.Freitas Jacenir R.Santos-Mallet AndréL.S.Santos Denise Feder Suzete A.O.Gomes Programa de Pós-Graduação em Ciências e Biotecnologia Instituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil Laboratório de Biodiversidade de Insetos e Patógenos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes Departamento de Microbiologia GeralInstituto de Microbiologia Paulo de GóesUniversidade Federal do Rio de JaneiroRJBrazil Fundação Oswaldo Cruz Piauí TeresinaPIBrazil Laboratório Interdisciplinar de Vigilância Entomológica em Díptera e Hemíptera Instituto Oswaldo CruzFundação Oswaldo CruzRio de JaneiroRJBrazil Universidade Iguaçu Nova IguaçuRJBrazil Laboratório de Biologia de Insetos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil

Objective:To investigate the morphological structure of ovarian follicular cells and biochemical parameters of both ovaries and fat bodies(sites of vitellogenesis)from Rhodnius(R.)prolixus infected with Trypanosoma(T.)rangeli.Methods:Adult virgin females of R.prolixus were fed upon a membrane apparatus containing heat-inactivated citrated rabbit blood and a suspension of T.rangeli epimastigotes(Macias strain).Females from the control group and all the males received parasite-free blood.Transmission electron microscopy was used to reveal the morphological aspects of ovarian follicle cells in both control and parasite-infected groups.Protein profile,proteolytic activities and Western blotting analyses were performed in either ovary or fat body samples of control and parasite-infected groups.Results:According to the ultrastructural data,T.rangeli infection elicited a degeneration process in the ovarian follicular cells of R.prolixus.Proteolytic assays indicated a reduction in the activity of aspartic peptidases in the ovary and fat body from parasite-infected group,while a significant increase in the cysteine peptidase activity was measured in both insect organs.Additionally,immunoblotting revealed that vitellogenin was overexpressed in the ovary of parasite-infected insects.Conclusions:T.rangeli infection seems to elicit an early programmed cell death in the ovarian follicle cells as well as induces the modulation on the activities of different peptidase classes in either ovaries or fat bodies and the overexpression of the vitellogenin in the ovary of R.prolixus.

关键词： Trypanosoma rangeli Rhodnius prolixus ovarian follicle Vitellogenin Aspartic protease Cysteine protease

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Apoptosis in Granulosa cells during follicular atresia: relationship with steroids and insulin-like growth factors

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Cell Research 2004年第4期14卷 341-346页

作者： Yuan Song YU, Hong Shu SUI, Zheng Bin HAN, Wei LI, Ming Jiu LUO, Jing He TAN Laboratory of Animal Reproduction and Embryology College of Animal Sciences and Veterinary Medicine, Shandong Agricultural University, Taian, Shandong 271018, China Present address: College of life science. University of Beijing, Beijing, China. Laboratory of Animal Reproduction and Embryology College of Animal Sciences and Veterinary Medicine Shandong Agricultural University Taian China College of life science University of Beijing Beijing China

It is well known that during mammalian ovarian follicular development, the majority of follicles undergo atresia at various stages of their development. However, the mechanisms controlling this selection process remain unknown. In this study, we investigated apoptosis in granulosa cells during goat follicular atresia by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The changes in the levels of steroids, insulin-like growth factors (IGFs) and IGF receptors were studied by radioimmunoassay (RIA) and semi-quantitative reverse transcrip-tion-PCR. We found that the percentage of apoptotic granulosa cells in the atretic (A) follicles was significantly higher than that in the slightly atretic (SA) and healthy (H) follicles. The level of estradiol and the ratio of estradiol to progesterone in H follicles were significantly higher than those in A follicles. On the other hand, the level of progesterone was not significantly different among these follicle types. We also found that t

关键词： apoptosis ovarian follicle steroids IGFs atresia.

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Changes of Serum Follicular Stimulating Hormone，Luteinizing Hormone and Follicular Sizes during Electroacupuncture for Induction of

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Chinese Journal of Integrative Medicine 1995年第2期1卷 13-16页

作者： Yu Jin (俞瑾) Zheng Huai-mei (郑怀美) and Bing Sheng-min (邴圣民)(Obstetrical and Gynecological Hospital, Shanghai Medical UniversityShanghai) (200011) Obstetrical and Gynecological Hospital Shanghai Medical University Shanghai

Changes in follicular stimulating hormone (FSH) . luteinizing hormone (LH) and follicularsizes were observed in 10 patients with chronic anovulation during electroacupuncture treatment. Sevencases were diagnosed as suffering from polycystic ovary syndrome, 2 from dysfunctional uterine bleeding,and 1 from hypogonadotropic amenorrhea. Among them 8 cases complained of infertility for an average of2. 7years. Ovulation was confirmed by pregnancy or the combination of biphasic basal body temperatureand ultrasonographic signs. During one course of 3 consecutive days of electroacupuncture treatment onacupoints Guanyuan (Ren 4), Zhongji (Ren 3), Zigong (Extra 16) and Sanyingjiao (SP 6). ovulation re-sulted in 5 patients (ovulating group) and 3 of the 4 infertile women became pregnant. Five cases failed toovulate (non-ovulating group) , 3 of them reached a biphasic basal body temperature without ovulatorysigns on ultrasonograph. Serum FSH, LH values and FSH pulse frequency increased significantly during-electroacupuncture treatment in the ovulating group (from 2. 10±0. 42 pulses/4h to 3. 70±1. 64 pulses/4h), but not in the non-ovulating group. No apparent change was found in LH pulse frequency, or in pulseamplitudes for FSH and LH. In the ovulating group, diameters of ovarian follicules markedly increased,but their growth was limited in the non-ovulation group. The results suggest ovulation may be induced byelectroacupuncture via a regulation on the hypothalamic-pituitary function, leading to normal secretion ofFSH and LH.

关键词： electroacupuncture treatment. pulsatile secretion ovarian follicle follicular stimulatinghormone. Iuteinizing hormone ovufation

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