Apoptosis in Granulosa cells during follicular atresia: relationship with steroids and insulin-like growth factors

作     者：Yuan Song YU, Hong Shu SUI, Zheng Bin HAN, Wei LI, Ming Jiu LUO, Jing He TAN Laboratory of Animal Reproduction and Embryology College of Animal Sciences and Veterinary Medicine, Shandong Agricultural University, Taian, Shandong 271018, China Present address: College of life science. University of Beijing, Beijing, China. 

作者机构：Laboratory of Animal Reproduction and Embryology College of Animal Sciences and Veterinary Medicine Shandong Agricultural University Taian China College of life science University of Beijing Beijing China 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2004年第14卷第4期

页      面：341-346页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 071009[理学-细胞生物学] 

基　　金：国家自然科学基金(30070556) 国家重点基础研究发展计划(973计划)(G200016107) 

主　　题：apoptosis ovarian follicle steroids IGFs atresia. 

摘      要：It is well known that during mammalian ovarian follicular development, the majority of follicles undergo atresia at various stages of their development. However, the mechanisms controlling this selection process remain unknown. In this study, we investigated apoptosis in granulosa cells during goat follicular atresia by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The changes in the levels of steroids, insulin-like growth factors (IGFs) and IGF receptors were studied by radioimmunoassay (RIA) and semi-quantitative reverse transcrip-tion-PCR. We found that the percentage of apoptotic granulosa cells in the atretic (A) follicles was significantly higher than that in the slightly atretic (SA) and healthy (H) follicles. The level of estradiol and the ratio of estradiol to progesterone in H follicles were significantly higher than those in A follicles. On the other hand, the level of progesterone was not significantly different among these follicle types. We also found that t