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The ANIP1-oswrky62 module regulates both basal defense and Pi9-mediated immunity against Magnaporthe oryzae in rice

Molecular Plant 2023年第4期16卷 739-755页

作者： Xuetao Shi Yehui Xiong Kai Zhang Yinshan Zhang Junqi Zhang Lili Zhang Yutao Xiao Guo-Liang Wang Wende Liu State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBejing 100193China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricutural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Department of Plant Pathology The Ohio State UniversityColumbusOH 43210USA

During effector-triggered immunity(ETI)against the devastating rice blast pathogen Magnaporthe oryzae,Pi9 functions as an intracellular resistance protein sensing the pathogen-secreted effector AvrPi9 in rice.Importantly,the underlying recognition mechanism(s)between Pi9 and AvrPi9 remains elusive.In this study,We identified a rice ubiquitin-like domain-containing protein(UDP),AVRPI9-INTERACTING PROTEIN 1(ANP1),which is directly targeted by AvrPi9 and also binds to Pi9 in plants.Phenotypic analysis of anip1 mu-tants and plants overexpressing ANIP1 revealed that ANIP1 negatively modulates rice basal defense against M.oryzae.ANiP1 undergoes 26S proteasome-mediated degradation,which can be blocked by both AvrPi9 and Pi9.Moreover,ANIP1 physically associates with the rice WRKY transcription factor oswrky62,which also interacts with AvrPi9 and Pi9 in plants.In the absence of Pi9,ANIP1 negatively regulates oswrky62 abundance,which can be promoted by AvrPi9.Accordingly,knocking out of oswrky62 in a non-Pi9 back-ground decreased immunity against M.oryzae.However,we also observed that oswrky62 plays negative roles in defense against a compatible M.oryzae strain in Pi9-harboring rice.Pi9 binds to ANiP1 and oswrky62 to form a complex,which may help to keep Pi9 in an inactive state and weaken rice immunity.Furthermore,using competitive binding assays,we showed that AvrPi9 promotes Pi9 dissociation from ANiP1,which could be an important step toward ETI activation.Taken together,our results reveal an immune strategy whereby a UDP-WRKY module,targeted by a fungal effector,modulates rice immunity in distinct ways in the presence or absence of the corresponding resistance protein.

关键词： AvrPi9 ANIP1 oswrky62 Pi9 rice blast disease immunity

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oswrky62 is a Negative Regulator of Basal and Xa21-Mediated Defense against Xanthomonas orvzae pv. orvzae in Rice

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Molecular Plant 2008年第3期1卷 446-458页

作者： Ying Peng Laura E. Bartley Xuewei Chen Christopher Dardick Mawsheng Chern Randy Ruan Patrick E. Canlas Pamela C. Ronald Department of Plant Pathology University of California Davis CA 95616 USA Present address: Monsanto Corporation Davis CA 95616 USA Present address: USDA-ARS Appalachian Fruit Research Station Kearneysville WV 25430 USA

The rice Xa21 gene, which confers resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae （Xoo）, encodes a receptor-like kinase, Few components involved in transducing the Xa21-mediated defense response have yet been identified. Here, we report that XA21 binds to a WRKY transcription factor, called oswrky62. The oswrky62 gene encodes two splice variants （oswrky62.1 and oswrky62.2）. oswrky62.1：smGFP2 and oswrky62.2：smGFP2 fusion pro- teins partially localize to the nucleus. Transgenic plants overexpressing oswrky62.1 are compromised in basal defense and Xa21-mediated resistance to Xoo. Furthermore, overexpression of oswrky62.1 suppresses the activation of defenserelated genes. These results imply that oswrky62 functions as a negative regulator of innate immunity in rice, and serves as a critical mediator of both basal and race-specific defense responses.

关键词： rice oswrky62 Xa21 defense response.

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