The ANIP1-OsWRKY62 module regulates both basal defense and Pi9-mediated immunity against Magnaporthe oryzae in rice
作者机构：State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBejing 100193China Shenzhen BranchGuangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricutural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Department of Plant PathologyThe Ohio State UniversityColumbusOH 43210USA
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2023年第16卷第4期
页 面：739-755页
核心收录：
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
基 金：supported by grants from the National Natural Science Foundation of China(31972229) the Agricultural Science and Technology Innovation Program(ASTIP) the Pests and Diseases Green Prevention and Control Major Special Project([110202101045([LS-05)] China Postdoctoral Science Foundation awards 2019M660893 and 2020T130710
主 题：AvrPi9 ANIP1 OsWRKY62 Pi9 rice blast disease immunity
摘 要：During effector-triggered immunity(ETI)against the devastating rice blast pathogen Magnaporthe oryzae,Pi9 functions as an intracellular resistance protein sensing the pathogen-secreted effector AvrPi9 in ***,the underlying recognition mechanism(s)between Pi9 and AvrPi9 remains *** this study,We identified a rice ubiquitin-like domain-containing protein(UDP),AVRPI9-INTERACTING PROTEIN 1(ANP1),which is directly targeted by AvrPi9 and also binds to Pi9 in *** analysis of anip1 mu-tants and plants overexpressing ANIP1 revealed that ANIP1 negatively modulates rice basal defense against ***1 undergoes 26S proteasome-mediated degradation,which can be blocked by both AvrPi9 and ***,ANIP1 physically associates with the rice WRKY transcription factor OsWRKY62,which also interacts with AvrPi9 and Pi9 in *** the absence of Pi9,ANIP1 negatively regulates OsWRKY62 abundance,which can be promoted by ***,knocking out of OsWRKY62 in a non-Pi9 back-ground decreased immunity against ***,we also observed that OsWRKY62 plays negative roles in defense against a compatible *** strain in Pi9-harboring ***9 binds to ANiP1 and OsWRKY62 to form a complex,which may help to keep Pi9 in an inactive state and weaken rice ***,using competitive binding assays,we showed that AvrPi9 promotes Pi9 dissociation from ANiP1,which could be an important step toward ETI *** together,our results reveal an immune strategy whereby a UDP-WRKY module,targeted by a fungal effector,modulates rice immunity in distinct ways in the presence or absence of the corresponding resistance protein.
维普期刊数据库